Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.
AuthorsGalla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C,
JournalNucleic Acids Res
PubMed ID21609958
'The Sleeping Beauty (SB) transposase and its newly developed hyperactive variant, SB100X, are of increasing interest for genome modification in experimental models and gene therapy. The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors delivered by physicochemical transfection or episomal retroviral vectors ... More
Protein transduction from retroviral Gag precursors.
AuthorsVoelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C,
JournalProc Natl Acad Sci U S A
PubMed ID20385817
Retroviral particles assemble a few thousand units of the Gag polyproteins. Proteolytic cleavage mediated by the retroviral protease forms the bioactive retroviral protein subunits before cell entry. We hypothesized that this process could be exploited for targeted, transient, and dose-controlled transduction of nonretroviral proteins into cultured cells. We demonstrate that ... More
AuthorsImberti B, Morigi M, Tomasoni S, Rota C, Corna D, Longaretti L, Rottoli D, Valsecchi F, Benigni A, Wang J, Abbate M, Zoja C, Remuzzi G,
JournalJ Am Soc Nephrol
PubMed ID17942965
In mice with cisplatin-induced acute kidney injury, administration of bone marrow-derived mesenchymal stem cells (MSC) restores renal tubular structure and improves renal function, but the underlying mechanism is unclear. Here, we examined the process of kidney cell repair in co-culture experiments with MSC and cisplatin-injured proximal tubular epithelial cells (PTEC). ... More
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts.
AuthorsShan L, Wang S, Sridhar R, Bhujwalla ZM, Wang PC
JournalMol Imaging
PubMed ID17445503
A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf(NIR)) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a ... More
Using total internal reflection fluorescence microscopy to visualize rhodopsin-containing cells.
Authors
JournalAppl Environ Microbiol
PubMed ID25769822
The human fetal adrenal produces cortisol but no detectable aldosterone throughout the second trimester.
Authors
JournalBMC Med
PubMed ID29429410
Role of Endoplasmic Reticulum Stress in Proinflammatory Cytokine-Mediated Inhibition of Trophoblast Invasion in Placenta-Related Complications of Pregnancy.
Authors
JournalAm J Pathol
PubMed ID30448406
Resveratrol reduces the proinflammatory effects and lipopolysaccharide- induced expression of HMGB1 and TLR4 in RAW264.7 cells.
Authors
JournalCell Physiol Biochem
PubMed ID24802390
Muscarinic receptors promote castration-resistant growth of prostate cancer through a FAK-YAP signaling axis.
Authors
JournalOncogene
PubMed ID32205868
HBV Facilitated Hepatocellular Carcinoma Cells Proliferation by Up-Regulating Angiogenin Expression Through IL-6.
Authors
JournalCell Physiol Biochem
PubMed ID29614505
A Drosophila model of oral peptide therapeutics for adult Intestinal Stem Cell tumors.
Authors
JournalDis Model Mech
PubMed ID34005044
A Drosophila model of oral peptide therapeutics for adult intestinal stem cell tumors.
Authors
JournalDis Model Mech
PubMed ID32540914
Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas.
Authors
JournalDevelopment
PubMed ID21989909
Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.