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View additional product information for SYTO™ Green Fluorescent Nucleic Acid Stains - FAQs (S32703, S7575, S7574, S7573, S7572, S7578, S7556, S34854, S7576, S34855, S7559)
6 product FAQs found
SYTO核酸染料的结合方式尚不清楚。但是，SYTO以及相关的核酸染料具有以下结合特性：
1.它们与一些溶剂结合（通过对盐、二价阳离子的敏感性，特别是SDS），因此，它们可能结合到DNA沟槽处。
2.所有SYTO染料都表现出一些碱基选择性，因此，它们可能结合到DNA小沟处。
3.通过乙醇沉淀可从核酸中去除SYTO染料；溴化乙锭和其他嵌入剂不具有此特性。同样，丁醇和氯仿提取不能从核酸中去除SYTO染料，但能够去除溴化乙锭。
4.SYTO 结合不受非离子去垢剂的影响。
5.SYTO染料不会被BrdU淬灭，因此，SYTO染料与核酸的结合方式不同于Hoechst 33342和DAPI（4',6-二脒基-2-苯基吲哚）。
SYBR Green I对移码指示菌几乎没有致突变性，证明其不大可能是强嵌入剂。
电子背景信号是由系统光学元件收集的杂散光和/或低水平电子信号造成的。背景信号可以通过正确设置仪器阈值和光电倍增管（PMT）电压而消除或降低。从其它微小颗粒和电子背景信号中区分细菌群落的最简单方法是用荧光染料标记细菌，然后使用该荧光来识别目标群落。SYTO 9是能够标记所有细胞的出色染料，可通过FITC通道检测。
The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:
1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid.
4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).
SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.
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SYTO 9 dye is one good choice for green; it is similar to fluorescein in excitation and emission wavelength. SYTO 59 dye is a good choice for red; it is similar to Texas Red. Both dyes are cell-permeant and stain DNA well, resulting in good nuclear labeling in live cells. With too high a concentration of dye and/or too long an incubation time, both may also label RNA, resulting in cytoplasmic and nucleolar staining, particularly with SYTO 59 dye.
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Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.
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Electronic noise is due to stray light collected by the system optics and/or low-level electronic signals. Noise may be eliminated or reduced through correct setup of instrument threshold and photomultiplier tube (PMT) voltage. The easiest way to differentiate a bacterial population from other small particles and electronic noise is to label the bacteria with a fluorescent stain and use the fluorescence emission to identify the population of interest. SYTO 9 is a great stain to label all cells and is detected in the FITC channel.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.