The evolutionarily conserved immune system of the zebrafish (Danio rerio), in combination with its genetic tractability, position it as an excellent model system in which to elucidate the origin and function of vertebrate immune cells. We recently reported the existence of antigen-presenting mononuclear phagocytes in zebrafish, namely macrophages and dendritic ... More
Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm.
AuthorsTawakoli PN, Al-Ahmad A, Hoth-Hannig W, Hannig M, Hannig C,
JournalClin Oral Investig
PubMed ID22821430
The aim of the present study was to investigate different fluorescence-based, two-color viability assays for visualization and quantification of initial bacterial adherence and to establish reliable alternatives to the ethidium bromide staining procedure. Bacterial colonization was attained in situ on bovine enamel slabs (n?=?6 subjects). Five different live/dead assays were ... More
Mechanisms of palatal epithelial seam disintegration by transforming growth factor (TGF) beta3.
AuthorsAhmed S, Liu CC, Nawshad A,
JournalDev Biol
PubMed ID17698055
TGFbeta3 signaling initiates and completes sequential phases of cellular differentiation that is required for complete disintegration of the palatal medial edge seam, that progresses between 14 and 17 embryonic days in the murine system, which is necessary in establishing confluence of the palatal stroma. Understanding the cellular mechanism of palatal ... More