Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green.
AuthorsFlors C, Fryer MJ, Waring J, Reeder B, Bechtold U, Mullineaux PM, Nonell S, Wilson MT, Baker NR
JournalJ Exp Bot
PubMed ID16595576
'Singlet oxygen is known to be produced by cells in response to photo-oxidative stresses and wounding. Due to singlet oxygen being highly reactive, it is thought to have a very short half-life in biological systems and, consequently, it is difficult to detect. A new commercially available reagent (singlet oxygen sensor ... More
Mechanisms of bacteriophage inactivation via singlet oxygen generation in UV illuminated fullerol suspensions.
'Nonenveloped viruses are shown to be inactivated by singlet oxygen ((1)O2) produced in UVA photosensitized aqueous suspensions of a polyhydroxylated fullerene (C60(OH)22-24; fullerol, 40 microM). Experiments were performed with MS2, a ssRNA bacteriophage, as well as two dsDNA phages: PRD1, which has an internal lipid membrane, and T7, which entirely ... More
Monitoring singlet oxygen and hydroxyl radical formation with fluorescent probes during photodynamic therapy.
AuthorsPrice M, Reiners JJ, Santiago AM, Kessel D,
JournalPhotochem Photobiol
PubMed ID19508643
'Singlet oxygen (1O2) is the primary oxidant generated in photodynamic therapy (PDT) protocols involving sensitizers resulting in type II reactions. 1O2 can give rise to additional reactive oxygen species (ROS) such as the hydroxyl radical (*OH). The current study was designed to assess 3''-p-(aminophenyl) fluorescein (APF) and 3''-p-(hydroxyphenyl) fluorescein (HPF) ... More
Singlet oxygen photosensitisation by the fluorescent probe Singlet Oxygen Sensor Green.
AuthorsRagàs X, Jiménez-Banzo A, Sánchez-García D, Batllori X, Nonell S,
JournalChem Commun (Camb)
PubMed ID19436910
The fluorescent probe Singlet Oxygen Sensor Green is able to produce singlet oxygen under exposure to UV or visible radiation.
Reactive oxygen species (ROS) are involved in many signalling pathways and numerous stress responses in plants. Consequently, it is important to be able to identify and localize ROS in vivo to evaluate their roles in signalling. A number of probes that have a high affinity for specific ROS and that ... More
Plasmonic engineering of singlet oxygen generation.
AuthorsZhang Y, Aslan K, Previte MJ, Geddes CD,
JournalProc Natl Acad Sci U S A
PubMed ID18252825
In this article, we report metal-enhanced singlet oxygen generation (ME(1)O2). We demonstrate a direct relationship between the singlet oxygen yield of a common photosensitizer (Rose Bengal) and the theoretical electric field enhancement or enhanced absorption of the photosensitizer in proximity to metallic nanoparticles. Using a series of photosensitizers, sandwiched between ... More
Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.
AuthorsFranco R, Panayiotidis MI, Cidlowski JA,
JournalJ Biol Chem
PubMed ID17724027
Changes in the intracellular redox environment of cells have been reported to be critical for the activation of apoptotic enzymes and the progression of programmed cell death. Glutathione (GSH) depletion is an early hallmark observed in apoptosis, and we have demonstrated that GSH efflux during death receptor-mediated apoptosis occurs via ... More
Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks.
Authors
JournalNat Commun
PubMed ID34857745
The plastoquinone pool outside the thylakoid membrane serves in plant photoprotection as a reservoir of singlet oxygen scavengers.
AuthorsKsas B, Légeret B, Ferretti U, Chevalier A, Pospíšil P, Alric J, Havaux M
JournalPlant Cell Environ
PubMed ID29601642
'The Arabidopsis vte1 mutant is devoid of tocopherol and plastochromanol (PC-8). When exposed to excess light energy, vte1 produced more singlet oxygen ('
A ruthenium(ii) based photosensitizer and transferrin complexes enhance photo-physical properties, cell uptake, and photodynamic therapy safety and efficacy.
AuthorsKaspler P, Lazic S, Forward S, Arenas Y, Mandel A, Lilge L
JournalPhotochem Photobiol Sci
PubMed ID26947517
'Metal-based photosensitizers are of interest as their absorption and chemical binding properties can be modified via the use of different ligands. Ru(2+) based photosensitizers are known to be effective photodynamic therapy (PDT) agents against bacteria, whereas use for oncological indications in vivo has not been demonstrated with the same level ... More
Ultrasmall Gd@Cdots as a radiosensitizing agent for non-small cell lung cancer.
Authors
JournalNanoscale
PubMed ID33982686
The Arabidopsis SAFEGUARD1 suppresses singlet oxygen-induced stress responses by protecting grana margins.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID32161131
Red Blood Cell-Facilitated Photodynamic Therapy for Cancer Treatment.
Authors
JournalAdv Funct Mater
PubMed ID31749670
Identification of distinct pH- and zeaxanthin-dependent quenching in LHCSR3 from Chlamydomonas reinhardtii.
Authors
JournalElife
PubMed ID33448262
Controlled gene and drug release from a liposomal delivery platform triggered by X-ray radiation.
Authors
JournalNat Commun
PubMed ID30006596
An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals.
Authors
JournalRedox Biol
PubMed ID32535542
Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.
Authors
JournalCell Chem Biol
PubMed ID32698018
X-ray induced singlet oxygen generation by nanoparticle-photosensitizer conjugates for photodynamic therapy: determination of singlet oxygen quantum yield.
AuthorsClement S, Deng W, Camilleri E, Wilson BC, Goldys EM
JournalSci Rep
PubMed ID26818819
Singlet oxygen is a primary cytotoxic agent in photodynamic therapy. We show that CeF3 nanoparticles, pure as well as conjugated through electrostatic interaction with the photosensitizer verteporfin, are able to generate singlet oxygen as a result of UV light and 8?keV X-ray irradiation. The X-ray stimulated singlet oxygen quantum yield ... More
Photodynamic therapy for Schistosoma mansoni: Promising outcomes.
Authorsde Melo NB, Dos Santos LFM, de Castro MS, Souza RLM, Marques MJ, Castro AP, de Castro AT, de Carli ML, Hanemann JAC, Silva MS, Moraes GOI, Beijo LA, Brigagão MRPL, Sperandio FF
JournalJ Photochem Photobiol B
PubMed ID29024873
The purpose of this study was to assess, for the very first time, the effects of photodynamic therapy (PDT) on Schistosoma mansoni in vitro by measuring reactive oxygen species (ROS) generation throughout the treatment, as well as the behavior of the parasites (mating, motility and contraction/shortening), and damage to their ... More
Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms.
AuthorsPrasad A, Sedlárová M, Pospíšil P
JournalSci Rep
PubMed ID30209276
Formation of singlet oxygen (
Photophysical properties and in vitro photocytotoxicity of disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX (Dimegine).
Photophysical and in vitro photocytotoxicity studies were performed for the photosensitizer Dimegine, a disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX with very low systemic toxicity. The singlet oxygen and luminescence quantum yield were F ... More
Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.
AuthorsOnukwufor JO, Trewin AJ, Baran TM, Almast A, Foster TH, Wojtovich AP
JournalFree Radic Biol Med
PubMed ID31841676
Fluorescent proteins can generate reactive oxygen species (ROS) upon absorption of photons via type I and II photosensitization mechanisms. The red fluorescent proteins KillerRed and SuperNova are phototoxic proteins engineered to generate ROS and are used in a variety of biological applications. However, their relative quantum yields and rates of ... More
Execution of programmed cell death by singlet oxygen generated inside the chloroplasts of Arabidopsis thaliana.
AuthorsAmbastha V, Chauhan G, Tiwari BS, Tripathy BC
JournalProtoplasma
PubMed ID31909436
Absorption of excess excitation energy induces overproduction of singlet oxygen (