SlowFade™ Glass Soft-set Antifade Mountant, 2 mL - FAQs

查看更多产品信息 SlowFade™ Glass Soft-Set Antifade Mountant - FAQs (S36917, S36917-5X2ML)

5 个常见问题解答

May I use nucleic acid stains for samples that will be mounted with SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917)?

Yes. SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) is compatible with many nucleic acid stains. Apply the stain at the last step prior to mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Will SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) preserve the structure of fluorescent proteins?

No. SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) does not maintain the structures of fluorescent proteins over time. The proteins will naturally unfold, and this unfolding would result in the loss of fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can be done to remove any tiny air bubbles in SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917)?

To remove tiny air bubbles, place the vial of SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) within a vacuum chamber with the vial cap loosened. Degas the solution by applying vacuum through either a T-connector on the faucet or a very brief application of vacuum from a vacuum pump.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) contain any volatile organic solvents?

No. SlowFade Glass Soft-set Antifade Mountant (Cat. No. S36917) is an aqueous-based mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.