4'-C-Trifluoromethyl modified oligodeoxynucleotides: synthesis, biochemical studies, and cellular uptake properties.
AuthorsZhou Y, Zang C, Wang H, Li J, Cui Z, Li Q, Guo F, Yan Z, Wen X, Xi Z, Zhou C
JournalOrg Biomol Chem
PubMed ID31112186
Herein, we report the synthesis of 4'-C-trifluoromethyl (4'-CF3) thymidine (T4'-CF3) and its incorporation into oligodeoxynucleotides (ODNs) through solid-supported DNA synthesis. The 4'-CF3 modification leads to a marginal effect on the deoxyribose conformation and a local helical structure perturbation for ODN/RNA duplexes. This type of modification slightly decreases the thermal stability ... More