Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors.
AuthorsChen D, Sun N, Hou L, Kim R, Faith J, Aslanyan M, Tao Y, Zheng Y, Fu J, Liu W, Kellis M, Clark A
JournalCell Rep
PubMed ID31875561
'In vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation of this model is the quality of the first progenitors called primordial germ cells (PGCs), which in vivo are specified during the peri-implantation window of human development. Here, we show that human PGC (hPGC) specification ... More
Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes.
AuthorsRwigemera A, Joao F, Delbes G
JournalEpigenetics Chromatin
PubMed ID28413450
'Epigenetic reprogramming is a critical step in male germ cell development that occurs during perinatal life. It is characterized by the remodeling of different epigenetic marks such as DNA methylation (5mC) and methylation of histone H3. It has been suggested that endocrine disruptors can affect the male germline epigenome by ... More
Biphasic ROS production, p53 and BIK dictate the mode of cell death in response to DNA damage in colon cancer cells.
AuthorsKutuk O, Aytan N, Karakas B, Kurt AG, Acikbas U, Temel SG, Basaga H
JournalPLoS One
PubMed ID28796811
'Necrosis, apoptosis and autophagic cell death are the main cell death pathways in multicellular organisms, all with distinct and overlapping cellular and biochemical features. DNA damage may trigger different types of cell death in cancer cells but the molecular events governing the mode of cell death remain elusive. Here we ... More
Altered chromatin compaction and histone methylation drive non-additive gene expression in an interspecific Arabidopsis hybrid.
AuthorsZhu W, Hu B, Becker C, Dogan ES, Berendzen KW, Weigel D, Liu C
JournalGenome Biol
PubMed ID28830561
'The merging of two diverged genomes can result in hybrid offspring that phenotypically differ greatly from both parents. In plants, interspecific hybridization plays important roles in evolution and speciation. In addition, many agricultural and horticultural species are derived from interspecific hybridization. However, the detailed mechanisms responsible for non-additive phenotypic novelty ... More
SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.
'Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits cell division while repairs are being made. In coccoids, such as the human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA as the staphylococcal SOS-induced ... More
Light needle microscopy with spatially transposed detection for axially resolved volumetric imaging.
AuthorsKozawa Y, Sato S
JournalSci Rep
PubMed ID31406209
'The demand for rapid three-dimensional volumetric imaging is increasing in various fields, including life science. Laser scanning fluorescence microscopy has been widely employed for this purpose; however, a volumetric image is constructed by two-dimensional image stacking with a varying observation plane, ultimately limiting the acquisition speed. Here we propose a ... More
Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia.
AuthorsBernier LP, Bohlen CJ, York EM, Choi HB, Kamyabi A, Dissing-Olesen L, Hefendehl JK, Collins HY, Stevens B, Barres BA, MacVicar BA
JournalCell Rep
PubMed ID31167136
'Microglia, the brain''s immune cells, maintain homeostasis and sense pathological changes by continuously surveying the parenchyma with highly motile large processes. Here, we demonstrate that microglia also use thin actin-dependent filopodia that allow fast nanoscale sensing within discrete regions. Filopodia are distinct from large processes by their size, speed, and ... More
SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research.
AuthorsHorikiri T, Ohi H, Shibata M, Ikeya M, Ueno M, Sotozono C, Kinoshita S, Sato T
JournalPLoS One
PubMed ID28107504
The neural crest is a source to produce multipotent neural crest stem cells that have a potential to differentiate into diverse cell types. The transcription factor SOX10 is expressed through early neural crest progenitors and stem cells in vertebrates. Here we report the generation of SOX10-Nano-lantern (NL) reporter human induced ... More
SVIP regulates Z variant alpha-1 antitrypsin retro-translocation by inhibiting ubiquitin ligase gp78.
AuthorsKhodayari N, Wang RL, Marek G, Krotova K, Kirst M, Liu C, Rouhani F, Brantly M
JournalPLoS One
PubMed ID28301499
Alpha-1 antitrypsin deficiency (AATD) is an inherited disorder characterized by early-onset emphysema and liver disease. The most common disease-causing mutation is a single amino acid substitution (Glu/Lys) at amino acid 342 of the mature protein, resulting in disruption of the 290-342 salt bridge (an electrophoretic abnormality defining the mutation [Z ... More
Plasmodium berghei Cap93, a novel oocyst capsule-associated protein, plays a role in sporozoite development.
AuthorsSasaki H, Sekiguchi H, Sugiyama M, Ikadai H
JournalParasit Vectors
PubMed ID28841886
Spatial organization of the somatosensory cortex revealed by osmFISH.
AuthorsCodeluppi S, Borm LE, Zeisel A, La Manno G, van Lunteren JA, Svensson CI, Linnarsson S
JournalNat Methods
PubMed ID30377364
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe ... More
easySLM-STED: Stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning.
AuthorsGörlitz F, Guldbrand S, Runcorn TH, Murray RT, Jaso-Tamame AL, Sinclair HG, Martinez-Perez E, Taylor JR, Neil MAA, Dunsby C, French PMW
JournalJ Biophotonics
PubMed ID29978591
We demonstrate a simplified set-up for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to ... More
Tunneling nanotubes, a novel mode of tumor cell-macrophage communication in tumor cell invasion.
AuthorsHanna SJ, McCoy-Simandle K, Leung E, Genna A, Condeelis J, Cox D
JournalJ Cell Sci
PubMed ID30659112
The interaction between tumor cells and macrophages is crucial in promoting tumor invasion and metastasis. In this study, we examined a novel mechanism of intercellular communication, namely membranous actin-based tunneling nanotubes (TNTs), that occurs between macrophages and tumor cells in the promotion of macrophage-dependent tumor cell invasion. The presence of ... More
Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts.
AuthorsYamamoto K, Otomo K, Nemoto T, Ishihara S, Haga H, Nagasaki A, Murakami Y, Takahashi M
JournalExp Cell Res
PubMed ID30711568
Nonmuscle myosin II (NMII) plays an important role in cytokinesis by constricting a contractile ring. However, it is poorly understood how NMII isoforms contribute to cytokinesis in mammalian cells. Here, we investigated the roles of the two major NMII isoforms, NMIIA and NMIIB, in cytokinesis using a WI-38 VA13 cell ... More
Immunofluorescence and image analysis pipeline for
AuthorsBrown JR, Phongthachit C, Sulkowski MJ
JournalBiol Methods Protoc
PubMed ID31403085
The neuromuscular junction (NMJ) of larval
Cellular and molecular assessment of rose bengal photodynamic antimicrobial therapy on keratocytes, corneal endothelium and limbal stem cell niche.
AuthorsNaranjo A, Pelaez D, Arrieta E, Salero-Coca E, Martinez JD, Sabater AL, Amescua G, Parel JM
JournalExp Eye Res
PubMed ID31539544
Rose Bengal Photodynamic Antimicrobial Therapy (RB-PDAT) is a novel potential treatment for progressive infectious keratitis. The principle behind this therapy is using Rose Bengal as a photosensitizer that can be activated by green light and results in the production of oxygen free radicals which in turn eradicate the microorganism. Given ... More
AuthorsIshii H, Otomo K, Hung JH, Tsutsumi M, Yokoyama H, Nemoto T
JournalBiomed Opt Express
PubMed ID31467771
We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup with electrically controllable components. For biological applications, we screened suitable fluorescent dyes or proteins and ... More