Fluorescent gramicidin derivatives for single-molecule fluorescence and ion channel measurements.
AuthorsLougheed T, Borisenko V, Hand CE, Woolley GA
JournalBioconjug Chem
PubMed ID11459465
'Single-molecule spectroscopies in combination with single-channel patch-clamp measurements have the potential for providing new information on ion channel gating processes. Fluorescent gramicidin derivatives could provide a means for calibrating such experiments since the structure of the open channel is known and the mechanism of gating (peptide dimerization) is generally agreed. ... More
Novel FRET-Based Assay to Detect Reverse Transcriptase Activity Using Modified dUTP Analogues.
AuthorsKrebs JF, Kore AR,
JournalBioconjug Chem
PubMed ID18163534
We have developed a novel continuous assay to measure reverse transcriptase (RT) polymerase activity. The assay uses fluorescence energy transfer measurements to detect the incorporation of complementary pairs of fluorescently labeled deoxyuridine into cDNA product. The fluorescently labeled dUTP substrates were prepared using commercially available reagents with a simple coupling ... More
An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays.
AuthorsFixe F, Chu V, Prazeres DM, Conde JP
JournalNucleic Acids Res
PubMed ID15148343
A flat microdevice which incorporates a thin-film amorphous silicon (a-Si:H) photodetector with an upper layer of functionalized SiO2 is used to quantify the density of both immobilized and hybridized DNA oligonucleotides labeled with a fluorophore. The device is based on the photoconductivity of hydrogenated amorphous silicon in a coplanar electrode ... More