Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.
AuthorsCarrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,
JournalJ Immunol
PubMed ID17548620
'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More
Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway.
AuthorsJung J, Yoon T, Choi EC, Lee K
JournalJ Biol Chem
PubMed ID12359716
'We reported previously that cofilin, an actin-binding protein, interacts with Na,K-ATPase and enhances its activity (Lee, K., Jung, J., Kim, M., and Guidotti, G. (2001) Biochem. J. 353, 377-385). To understand the nature of this interaction and the role of cofilin in the regulation of Na,K-ATPase activity, we searched for ... More
Na+-dependent release of Mg2+ from an intracellular pool in rat sublingual mucous acini.
AuthorsZhang GH, Melvin JE
JournalJ Biol Chem
PubMed ID8910560
'Muscarinic stimulation induces release of Mg2+ from an intracellular pool in rat sublingual mucous acini (Zhang, G. H., and Melvin, J. E. (1992) J. Biol. Chem. 267, 20721-20727). In the present study we examined the interdependence of Mg2+ mobilization on intracellular Na+ and Ca2+ by monitoring the intracellular free concentrations ... More
Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier.
AuthorsJiang J, Song Y, Bai C, Koller BH, Matthay MA, Verkman AS
JournalJ Appl Physiol
PubMed ID12391048
'We developed a pleural surface fluorescence method to measure Na(+) and Cl(-) transport in perfused mouse lungs. The air space was filled with aqueous fluid containing membrane-impermeant fluorescent indicators of Cl(-) (lucigenin) or Na(+) (Sodium Green). After instillation of a Cl(-)-free solution into the air space, an increase in perfusate ... More
Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.
AuthorsLo CJ, Leake MC, Berry RM
JournalBiophys J
PubMed ID16227503
'The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular ... More
Intracellular Na+ measurements using sodium green tetraacetate with flow cytometry.
AuthorsAmorino GP, Fox MH
JournalCytometry
PubMed ID8582247
'Intracellular sodium concentration ([Na+]i) can be measured using SBFI with image analysis or spectrofluorometry. However, SBFI cannot be used with most flow cytometry systems, due to its requirement for dual excitation in the UV range. Recently a new sodium indicator, Sodium Green, was developed which can be used with flow ... More
Permeation properties of a P2X receptor in the green algae Ostreococcus tauri.
AuthorsFountain SJ, Cao L, Young MT, North RA,
JournalJ Biol Chem
PubMed ID18381285
'We have cloned a P2X receptor (OtP2X) from the green algae Ostreococcus tauri. The 42-kDa receptor shares approximately 28% identity with human P2X receptors and 23% with the Dictyostelium P2X receptor. ATP application evoked flickery single channel openings in outside-out membrane patches from human embryonic kidney 293 cells expressing OtP2X. ... More
Use of confocal microscopy to investigate cell structure and function.
AuthorsBkaily G, Jacques D, Pothier P
JournalMethods Enzymol
PubMed ID10506971
The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells.
AuthorsBkaily G, Pothier P, D'Orléans-Juste P, Simaan M, Jacques D, Jaalouk D, Belzile F, Hassan G, Boutin C, Haddad G, Neugebauer W
JournalMol Cell Biochem
PubMed ID9278244
In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various ... More
Anoxia induces an increase in intracellular sodium in rat central neurons in vitro.
AuthorsFriedman JE, Haddad GG
JournalBrain Res
PubMed ID7874519
Following our previous observations that anoxia induces a drop in extracellular Na+ in the brain slice and that removal of extracellular Na+ prevents the anoxia-induced morphological changes in dissociated hippocampal neurons, we hypothesized that intracellular Na+ increases during anoxia in isolated neurons. Using the fluorophore Sodium Green in freshly dissociated ... More
Alteration of intracellular potassium and sodium concentrations correlates with induction of cytopathic effects by human immunodeficiency virus.
Increases in intracellular concentrations of potassium ([K+]i) and sodium ([Na+]i) occur concomitantly with cytopathic effects induced in a CD4+ T-lymphoblastoid cell line acutely infected by human immunodeficiency virus (HIV). This [K+]i increase was greater in cells infected by cytopathic HIV strains than in cells infected by less cytopathic strains. T ... More
Visualization in zebrafish larvae of Na(+) uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a.
Uptake of Na(+) from the environment is an indispensable strategy for the survival of freshwater fish, as they easily lose Na(+) from the plasma to a diluted environment. Nevertheless, the location of and molecules involved in Na(+) uptake remain poorly understood. In this study, we utilized Sodium Green, a Na(+)-dependent ... More
PINK1-associated Parkinson's disease is caused by neuronal vulnerability to calcium-induced cell death.
AuthorsGandhi S, Wood-Kaczmar A, Yao Z, Plun-Favreau H, Deas E, Klupsch K, Downward J, Latchman DS, Tabrizi SJ, Wood NW, Duchen MR, Abramov AY,
JournalMol Cell
PubMed ID19285945
Mutations in PINK1 cause autosomal recessive Parkinson's disease. PINK1 is a mitochondrial kinase of unknown function. We investigated calcium homeostasis and mitochondrial function in PINK1-deficient mammalian neurons. We demonstrate physiologically that PINK1 regulates calcium efflux from the mitochondria via the mitochondrial Na(+)/Ca(2+) exchanger. PINK1 deficiency causes mitochondrial accumulation of calcium, ... More
Deglycosylation of Na+/K+-ATPase causes the basolateral protein to undergo apical targeting in polarized hepatic cells.
AuthorsLian WN, Wu TW, Dao RL, Chen YJ, Lin CH
JournalJ Cell Sci
PubMed ID16339171
Polarized epithelia, such as hepatocytes, target their integral membrane proteins to specific apical or basolateral membrane domains during or after biogenesis. The roles played by protein glycosylation in this sorting process remain controversial. We report here that deglycosylation treatments in well-polarized hepatic cells by deglycosylation drugs, or by site-directed mutagenesis ... More
Effects of hyperthermia on intracellular chloride.
AuthorsAmorino GP, Fox MH
JournalJ Membr Biol
PubMed ID8672083
Hyperthermia induces transient changes in [Na+]i and [K+]i in mammalian cells. Since Cl- flux is coupled with Na+ and K+ in several processes, including cell volume control, we have measured the effects of heat on [Cl-]i using the chloride indicator, MQAE, with flow cytometry. The mean basal level of [Cl-]i ... More
Fluorescence lifetime microscopy of the Na+ indicator Sodium Green in HeLa cells.
AuthorsDespa S, Vecer J, Steels P, Ameloot M
JournalAnal Biochem
PubMed ID10870831
This study investigates the usefulness of lifetime measurements of Sodium Green for evaluating intracellular Na+ concentration ([Na+]i) in HeLa cells. Frequency-domain lifetime measurements are performed in HeLa cells and in different buffer solutions (with and without K+ and bovine serum albumin). In all cases, the fluorescence decays of Sodium Green ... More
Differential effects of stretch and compression on membrane currents and [Na+]c in ventricular myocytes.
AuthorsIsenberg G, Kazanski V, Kondratev D, Gallitelli MF, Kiseleva I, Kamkin A
JournalProg Biophys Mol Biol
PubMed ID12732267
Mechano-electrical feedback was studied in the single ventricular myocytes. A small fraction (approximately 10%) of the cell surface could be stretched or compressed by a glass stylus. Stretch depolarised, shortened the action potential and induced extra systoles. Stretch activated non-selective cation currents (I(ns)) showed a linear voltage dependence, a reversal ... More
Heat-induced changes in intracellular sodium and membrane potential: lack of a role in cell killing and thermotolerance.
AuthorsAmorino GP, Fox MH
JournalRadiat Res
PubMed ID8752306
Hyperthermia induces transient changes in intracellular free sodium levels and membrane potential. The possible role of these changes in cell killing by hyperthermia and thermotolerance has been evaluated using Chinese hamster ovary IS1 and HeLa cells. Intracellular sodium was measured with Sodium Green and SBFI, while membrane potential was measured ... More
Extracellular ATP induces oscillations of intracellular Ca2+ and membrane potential and promotes transcription of IL-6 in macrophages.
AuthorsHanley PJ, Musset B, Renigunta V, Limberg SH, Dalpke AH, Sus R, Heeg KM, Preisig-Müller R, Daut J
JournalProc Natl Acad Sci U S A
PubMed ID15194822
The effects of low concentrations of extracellular ATP on cytosolic Ca(2+), membrane potential, and transcription of IL-6 were studied in monocyte-derived human macrophages. During inflammation or infection many cells secrete ATP. We show here that application of 10 microM ATP or 10 microM UTP induces oscillations in cytosolic Ca(2+) with ... More
Regulation of Na+,K+-ATPase by persistent sodium accumulation in adult rat thalamic neurones.
AuthorsSenatorov VV, Stys PK, Hu B
JournalJ Physiol
PubMed ID10835038
The present study investigated the regulatory mechanism of the Na+, K+-ATPase and the level of internal Na+ and Ca2+ in response to persistent Na+ influx in acutely dissociated rat thalamic neurones. Whole-cell patch-clamp recordings and Na+ imaging revealed a stable [Na+]i and low background pump activity. Exposure to veratridine (50 ... More
Sodium and epithelial sodium channels participate in the regulation of the capacitation-associated hyperpolarization in mouse sperm.
AuthorsHernández-González EO, Sosnik J, Edwards J, Acevedo JJ, Mendoza-Lujambio I, López-González I, Demarco I, Wertheimer E, Darszon A, Visconti PE
JournalJ Biol Chem
PubMed ID16407190
In a process called capacitation, mammalian sperm gain the ability to fertilize after residing in the female tract. During capacitation the mouse sperm plasma membrane potential (E(m)) hyperpolarizes. However, the mechanisms that regulate sperm E(m) are not well understood. Here we show that sperm hyperpolarize when external Na(+) is replaced ... More
Gender influences [Ca(2+)](i) during metabolic inhibition in myocytes overexpressing the Na(+)-Ca(2+) exchanger.
AuthorsSugishita K, Su Z, Li F, Philipson KD, Barry WH
JournalCirculation
PubMed ID11673353
BACKGROUND: The Na(+)-Ca(2+) exchanger (NCX) may contribute to Ca(2+) overload and injury in ischemic cardiomyocytes. Recently, NCX overexpression was reported to increase ischemia/reperfusion injury in male and oophorectomized female but not in female mice. We therefore measured the effects of gender and estrogen on [Ca(2+)](i) and [Na(+)](i) during metabolic inhibition ... More
Hemolysis of erythrocytes by granulysin-derived peptides but not by granulysin.
AuthorsLi Q, Dong C, Deng A, Katsumata M, Nakadai A, Kawada T, Okada S, Clayberger C, Krensky AM
JournalAntimicrob Agents Chemother
PubMed ID15616319
Granulysin, a 9-kDa protein localized in human cytolytic T lymphoctyes and natural killer cell granules, is cytolytic against tumors and microbes but not against red blood cells. Synthetic peptides corresponding to the central region of granulysin recapitulate the lytic activity of the intact molecule, and some peptides cause hemolysis of ... More
Amphetamine-induced dopamine efflux. A voltage-sensitive and intracellular Na+-dependent mechanism.
AuthorsKhoshbouei H, Wang H, Lechleiter JD, Javitch JA, Galli A
JournalJ Biol Chem
PubMed ID12556446
Amphetamine (AMPH) elicits its behavioral effects by acting on the dopamine (DA) transporter (DAT) to induce DA overflow into the synaptic cleft. Facilitated exchange diffusion is the classical model used to describe AMPH-induced DA efflux. This model hypothesizes that AMPH-induced DA efflux is mediated by DAT and results from the ... More
Activation of connexin-43 hemichannels can elevate [Ca(2+)]i and [Na(+)]i in rabbit ventricular myocytes during metabolic inhibition.
AuthorsLi F, Sugishita K, Su Z, Ueda I, Barry WH
JournalJ Mol Cell Cardiol
PubMed ID11735261
ATP depletion due to ischemia or metabolic inhibition (MI) causes Na(+) and Ca(2+) accumulation in myocytes, which may be in part due to opening of connexin-43 hemichannels. Halothane (H) has been shown to reduce conductance of connexin-43 hemichannels and to protect the heart against ischemic injury. We therefore investigated the ... More
Bile acids induce a cationic current, depolarizing pancreatic acinar cells and increasing the intracellular Na+ concentration.
AuthorsVoronina SG, Gryshchenko OV, Gerasimenko OV, Green AK, Petersen OH, Tepikin AV
JournalJ Biol Chem
PubMed ID15536077
Biliary disease is a major cause of acute pancreatitis. In this study we investigated the electrophysiological effects of bile acids on pancreatic acinar cells. In perforated patch clamp experiments we found that taurolithocholic acid 3-sulfate depolarized pancreatic acinar cells. At low bile acid concentrations this occurred without rise in the ... More
Regulation of [Na+]i in resting and stimulated submandibular salivary ducts.
AuthorsXu X, Zhao H, Diaz J, Muallem S
JournalJ Biol Chem
PubMed ID7642648
In the preceding manuscript (Zhao, H., Xu, X., Diaz, J., and Muallem, S. (1995) J. Biol. Chem. 270, 19599-19605), we described a Kout(+)-dependent H+/HCO3- and Na+ influx pathway in the luminal membrane of salivary duct cells. In the present studies, we further characterized this pathway to show that the Kout(+)-dependent ... More
The soybean plasma membrane-localized cation/H+ exchanger GmCHX20a plays a negative role under salt stress.
Authors
JournalPhysiol Plant
PubMed ID33094482
Tonoplast-located GmCLC1 and GmNHX1 from soybean enhance NaCl tolerance in transgenic bright yellow (BY)-2 cells.
Authors
JournalPlant Cell Environ
PubMed ID17080938
The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Authors
JournalSci Rep
PubMed ID26726070
Otx2b mutant zebrafish have pituitary, eye and mandible defects that model mammalian disease.
Authors
JournalHum Mol Genet
PubMed ID32277752
GmSAL1 hydrolyzes inositol-1,4,5-trisphosphate and regulates stomatal closure in detached leaves and ion compartmentalization in plant cells.
Authors
JournalPLoS One
PubMed ID24167607
Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis.
Authors
JournalPlant Cell Physiol
PubMed ID19880402
Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL.
Authors
JournalFront Endocrinol (Lausanne)
PubMed ID30515132
Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis.