Use of a fluorescent internal protein standard to achieve quantitative two-dimensional gel electrophoresis.
AuthorsWheelock AM, Morin D, Bartosiewicz M, Buckpitt AR
JournalProteomics
PubMed ID16429456
2-DE is a powerful separation method for complex protein mixtures. However, large intergel variations in spot intensity limit its use for quantitative proteomics studies. To address this issue, we developed a fluorescent internal protein standard for use in 2-DE analysis. Protein samples are spiked with an Alexa-labeled internal standard (ALIS) ... More