Cellular reactivity to mechanical axonal injury in an organotypic in vitro model of neurotrauma.
AuthorsSieg F, Wahle P, Pape HC
JournalJ Neurotrauma
PubMed ID10619198
'An in vitro model of traumatic brain injury is described that is based on organotypic cocultures (OTCs) of rat neocortex and thalamus connected by reciprocal axonal projections. Localized mechanical compression of this projection was inflicted with a mechanical device, and the effects on cell viability, axonal morphology, and protein expression ... More
Sarcocystis neurona and Sarcocystis falcatula: monitoring of schizogony in cell culture using fluorescent nuclear labeling.
The nuclei of merozoites of Sarcocystis neurona and Sarcocystis falcatula were labeled with the fluorescent marker Syto21. It was shown that the marker would label the parasites and that they would retain the marker throughout schizogony. Thus, there was sufficient marker in the daughter merozoites to make them easily visible ... More
Design of helical proteins for real-time endoprotease assays.
AuthorsSteinrücke P, Aldinger U, Hill O, Hillisch A, Basch R, Diekmann S
JournalAnal Biochem
PubMed ID11038269
Proteases play a key role in cellular biology and have become priority targets for new pharmaceuticals. Thus, there is a high demand for specific, sensitive, and quick assays to monitor the activity of endoproteases. We designed affinity-tagged helical proteins with unique protease cleavage sites and thus constructed universal, molecularly defined, ... More
Genetically programmed alternative splicing of NEMO mediates an autoinflammatory disease phenotype.
Authors
JournalJ Clin Invest
PubMed ID35289316
Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms.
Authors
JournalDev Cell
PubMed ID31564612
High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL).