SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO, 500 μL - FAQs

View additional product information for SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO - FAQs (S7564, S7586, S7568)

12 product FAQs found

SYBR染料的pH范围是多少？

SYBR染料仅在很窄的pH范围内有效，约为pH 7-8。超出此范围，荧光信号将迅速消失。

推荐凝胶成像系统使用什么滤光片？

请点击此处(https://www.thermofisher.com/cn/zh/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html)并选择“滤光片推荐”，查看适用于SYBR Safe DNA凝胶染料的推荐滤光片。请注意，SYBR Safe DNA凝胶染料的激发和发射光谱与SYBR Green I、SYBR Green II、SYBR Gold 染料，还有荧光素（FITC）极为相似。因此，亦可使用适用于这些染料的滤光片。若使用Safe Imager蓝光透射仪，则无需使用相机滤光片；仪器内提供的琥珀色滤光片即具有该功能。

我能否在我的相机上使用溴化乙锭滤光片对SYBR染料进行成像吗？

不推荐。大部分深琥珀/橙色溴化乙锭滤光片在550nm附近有临界值。SYBR Green染料在520nm处有发射光，所以使用这种滤光片会过滤掉您所需要的数据。

SYBR Safe DNA凝胶染料与SYBR Green I染料相同吗？

所有SYBR染料都具有相似的光谱性质，但化学组成不同。所有SYBR染料都与dsDNA、ssDNA和RNA结合，但敏感性不同。SYBRSafe DNA凝胶染料是专门研发的溴化乙锭安全替代品。SYBR Green I是dsDNA的超灵敏染料，而SYBR Green II是RNA和ssDNA的高灵敏性染料。所有SYBR染料都适用于Safe Imager蓝光透射仪激发观察。

各种SYBR染料有何区别？

所有SYBR染料都与dsDNA、ssDNA和RNA结合，但敏感性不同。SYBR Green I用于dsDNA和ssDNA染色。SYBR Green II可以对dsDNA和ssDNA进行染色，但对RNA的敏感性更好。SYBR Gold 是在SYBR Green I和II之后开发的，是最敏感的荧光凝胶染色剂。SYBR Safe DNA凝胶染色剂是一种用于蓝光系统的致突变性更低的配方。它不像SYBR Green I和II那么敏感，但可与溴化乙锭相比。 < / p >

我能否对TBE凝胶染色？如何染色？

是的，您可以使用溴化乙锭、SYBR Green I、SYBR Green II和SilverXpress银染试剂盒对TBE凝胶染色。采用溴化乙锭染色时，将胶侵泡在使用超纯水配制的2 µg/mL溴化乙锭溶液中20分钟。染色后使用超纯水连续冲洗3次，每次10分钟，除去未结合染料。在紫外灯下观察条带。

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.