SYBR™ Photographic Filter

SYBR™ Photographic Filter - FAQs

2 个常见问题解答

各种SYBR染料有何区别？

所有SYBR染料都与dsDNA、ssDNA和RNA结合，但敏感性不同。SYBR Green I用于dsDNA和ssDNA染色。SYBR Green II可以对dsDNA和ssDNA进行染色，但对RNA的敏感性更好。SYBR Gold 是在SYBR Green I和II之后开发的，是最敏感的荧光凝胶染色剂。SYBR Safe DNA凝胶染色剂是一种用于蓝光系统的致突变性更低的配方。它不像SYBR Green I和II那么敏感，但可与溴化乙锭相比。 < / p >

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.