A cluster of noninvoluting endocytic cells at the margin of the zebrafish blastoderm marks the site of embryonic shield formation.
AuthorsCooper MS,D'Amico LA
JournalDevelopmental biology
PubMed ID8948584
Abnormal regulation of retinoic acid receptor beta2 expression and compromised allograft rejection in transgenic mice expressing antisense sequences to retinoic acid receptor beta1 and beta3.
Transgenic mice carrying antisense sequences common to the retinoic acid receptors (RAR) beta1 and beta3 were produced to examine roles of RARbeta1 and beta3 in the immune system. There were no significant changes of endogenous RARbeta1/beta3 expression at the mRNA level in T cells, B cells, and macrophages of the ... More
Nucleic acid dyes for detection of apoptosis in live cells.
AuthorsFrey T
JournalCytometry
PubMed ID8582249
'Apoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser. The collection of dyes giving this staining pattern includes reagents ... More
Multiparameter detection of apoptosis using red-excitable SYTO probes.
'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More
Cleavage orientation and the asymmetric inheritance of Notch1 immunoreactivity in mammalian neurogenesis.
AuthorsChenn A, McConnell SK
JournalCell
PubMed ID7664342
'Neurons in the mammalian central nervous system are generated from progenitor cells near the lumen of the neural tube. Time-lapse microscopy of dividing cells in slices of developing cerebral cortex reveals that cleavage orientation predicts the fates of daughter cells. Vertical cleavages produce behaviorally and morphologically identical daughters that resemble ... More
Oxidized LDLs induce massive apoptosis of cultured human endothelial cells through a calcium-dependent pathway. Prevention by aurintricarboxylic acid.
AuthorsEscargueil-Blanc I, Meilhac O, Pieraggi MT, Arnal JF, Salvayre R, Nègre-Salvayre A
JournalArterioscler Thromb Vasc Biol
PubMed ID9081689
'Oxidized LDLs are thought to play a central role in atherogenesis. Among their wide variety of biological properties, oxidized LDLs exhibit a cytotoxic effect on cultured vascular cells. Toxic doses of mildly oxidized LDLs elicited massive apoptosis in both primary and immortalized cultures of endothelial cells as shown by characteristic ... More
Proteins with glycosylphosphatidylinositol (GPI) signal sequences have divergent fates during a GPI deficiency. GPIs are essential for nuclear division in Trypanosoma cruzi.
AuthorsGarg N, Tarleton RL, Mensa-Wilmot K
JournalJ Biol Chem
PubMed ID9139697
'Glycosylphosphatidylinositols (GPIs) are membrane anchors for cell surface proteins of several major protozoan parasites of humans, including Trypanosoma cruzi, the causative agent of Chagas'' disease. To investigate the general role of GPIs in T. cruzi, we generated GPI-deficient parasites by heterologous expression of T. brucei GPI-phospholipase C. Putative protein-GPI intermediates ... More
Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry.
AuthorsPoot M, Gibson LL, Singer VL
JournalCytometry
PubMed ID9098628
'We characterized the ability of six SYTO nucleic acid stains and a mitochondrial stain to resolve by flow cytometry camptothecin-induced apoptotic and non-apoptotic cells. Staining live human lymphoid B-cells showed such resolution with SYTO 11, 12, 13, 14, and 16 dyes. H9, HL-60, and Jurkat cells did not show resolution ... More
Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
AuthorsLebaron P, Parthuisot N, Catala P
JournalAppl Environ Microbiol
PubMed ID9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More
Analyzing morphogenetic cell behaviors in vitally stained zebrafish embryos.
AuthorsCooper MS, D'Amico LA, Henry CA
JournalMethods Mol Biol
PubMed ID10231793
Use of confocal microscopy to investigate cell structure and function.
AuthorsBkaily G, Jacques D, Pothier P
JournalMethods Enzymol
PubMed ID10506971
The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells.
AuthorsBkaily G, Pothier P, D'Orléans-Juste P, Simaan M, Jacques D, Jaalouk D, Belzile F, Hassan G, Boutin C, Haddad G, Neugebauer W
JournalMol Cell Biochem
PubMed ID9278244
In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various ... More
NUC-1, a caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis.
AuthorsWu YC, Stanfield GM, Horvitz HR
JournalGenes Dev
PubMed ID10716942
One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation in ... More
Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons.
AuthorsFrancis F, Koulakoff A, Boucher D, Chafey P, Schaar B, Vinet MC, Friocourt G, McDonnell N, Reiner O, Kahn A, McConnell SK, Berwald-Netter Y, Denoulet P, Chelly J
JournalNeuron
PubMed ID10399932
Recently, we and others reported that the doublecortin gene is responsible for X-linked lissencephaly and subcortical laminar heterotopia. Here, we show that Doublecortin is expressed in the brain throughout the period of corticogenesis in migrating and differentiating neurons. Immunohistochemical studies show its localization in the soma and leading processes of ... More
SYTO probes in the cytometry of tumor cell death.
AuthorsWlodkowic D, Skommer J, Darzynkiewicz Z,
JournalCytometry A
PubMed ID18260152
Apoptosis is a complex and finely controlled cell death process of great relevance in tissue homeostasis and pathogenesis. The majority of classical apoptotic features can be examined by flow as well as image cytometry. Therefore, cytometry has been used as a technology of choice in studies of tumor cell demise. ... More
Quantification of neurotoxicity and identification of cellular subsets in a three-dimensional brain model.
AuthorsPulliam L, Stubblebine M, Hyun W
JournalCytometry
PubMed ID9581626
Imaging of cells in a large intact three-dimensional tissue remains difficult. Quantification and identification of cell damage in a mixed culture system has been limited by the inability of fluorescent probes to discriminate types of cellular death and penetrate tissue more that 100 microm thick. We have investigated several probes ... More
Electrophoretic behavior of individual nuclear species as determined by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsGunasekera N, Musier-Forsyth K, Arriaga E
JournalElectrophoresis
PubMed ID12210266
We determined the feasibility of using capillary electrophoresis with postcolumn laser-induced fluorescence (CE-LIF) detection to characterize electrophoretic properties of isolated cell nuclei and impurities present in nuclear fractions. These fractions were isolated from NS-1 mouse hybridoma cells, stained with hexidium iodide, a DNA intercalating dye, and analyzed by CE-LIF detection. ... More
Confocal imaging of microglial cell dynamics in hippocampal slice cultures.
AuthorsDailey ME, Waite M
JournalMethods
PubMed ID10356354
Methods are described for imaging the cellular dynamics of microglia in live mammalian brain slice cultures. Brain slices prepared from developing rat hippocampus are cultured for up to 2 weeks by the roller tube or static filter culture technique, stained with one or more fluorescent dyes, and imaged by scanning ... More
Rac1 and Cdc42 regulate hyphal growth and cytokinesis in the dimorphic fungus Ustilago maydis.
AuthorsMahlert M, Leveleki L, Hlubek A, Sandrock B, Bölker M
JournalMol Microbiol
PubMed ID16390450
Small GTP-binding proteins of the highly conserved Rho family act as molecular switches regulating cell signalling, cytoskeletal organization and vesicle trafficking in eukaryotic cells. Here we show that in the dimorphic plant pathogenic fungus Ustilago maydis deletion of either cdc42 or rac1 results in loss of virulence but does not ... More
Formation of planar and spiral Ca2+ waves in isolated cardiac myocytes.
AuthorsIshida H, Genka C, Hirota Y, Nakazawa H, Barry WH
JournalBiophys J
PubMed ID10512831
A novel Nipkow-type confocal microscope was applied to image spontaneously propagating Ca2+ waves in isolated rat ventricular myocytes by means of fluo-3. The sarcolemma was imaged with di-8-ANEPPS and the nucleus with SYTO 11. Full frame images in different vertical sections were obtained at video frame rate by means of ... More
A double-label technique that monitors sulfur mustard damage to nuclei and mitochondria of normal human epidermal keratinocytes in vitro.
AuthorsCook JR, Van Buskirk RG
JournalToxicol Pathol
PubMed ID9323838
Sulfur mustard and 2-chloro ethyl ethyl sulfide (CEES, a sulfur mustard analog) is known to have immediate (minutes), long-term (hours to days), and toxic effects on human skin. Research was directed toward developing a single in vitro assay that might reflect both these short-term and long-term effects of this vesicating ... More
Bradykinin activates R-, T-, and L-type Ca2+ channels and induces a sustained increase of nuclear Ca2+ in aortic vascular smooth muscle cells.
AuthorsBkaily G, Jaalouk D, Jacques D, Economos D, Hassan G, Simaan M, Regoli D, Pothier P
JournalCan J Physiol Pharmacol
PubMed ID9276144
The mechanism(s) fo Ca2+ entry stimulated by bradykinin (BK) and the receptor subtype responsible for this effect were examined in human and rabbit aortic vascular smooth muscle cells (VSMCs). Using the whole-cell voltage clamp technique, BK (10(-6)M) significantly (p < 0.05) increased both T- and L-type Ca2+ currents (ICa) in ... More
Confocal microscopic analysis of morphogenetic movements.
AuthorsCooper MS, D'Amico LA, Henry CA
JournalMethods Cell Biol
PubMed ID9891361
Confocal microscopy is an excellent means of imaging cellular dynamics within living zebrafish embryos because it provides a means of optically sectioning tissues that have been labeled with specific fluorescent probe molecules. In order to study genetically encoded patterns of cell behavior that are involved in the formation of germ ... More
Nuclear Ca2+ signaling to endothelin-1 in rat aortic smooth-muscle cells.
AuthorsNaik RD, McNeill JR, Wilson TW, Gopalakrishnan V
JournalJ Cardiovasc Pharmacol
PubMed ID9595437
Vascular smooth-muscle cells (VSMCs) isolated from genetically hypertensive animals show increased intracellular free calcium levels ([Ca2+]i) in response to endothelin-1 (ET-1). The differences in time course and distribution of Ca2+ increase after addition of ET-1 within the VSMCs are unknown. Therefore, ET-1-evoked changes in fluo-3 fluorescence were determined using a ... More
Lipid bodies and lipid body formation in an oleaginous fungus, Mortierella ramanniana var. angulispora.
AuthorsKamisaka Y, Noda N, Sakai T, Kawasaki K
JournalBiochim Biophys Acta
PubMed ID10320801
Mortierella ramanniana var. angulispora accumulates triacylglycerol (TG) in lipid bodies. Studies on lipid transport into lipid bodies are essential for elucidating mechanisms of lipid body formation. We used fluorescent dyes and fluorescent lipid analogs to visualize lipid body formation with a confocal laser scanning microscope. Different sizes of lipid bodies ... More
Artemisinin exposure at the ring or trophozoite stage impacts Plasmodium falciparum sexual conversion differently.
Authors
JournalElife
PubMed ID33084568
Zinc-Containing Effluent Treatment Using Shewanella xiamenensis Biofilm Formed on Zeolite.
Authors
JournalMaterials (Basel)
PubMed ID33918384
Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum.
Authors
JournalSci Rep
PubMed ID31601834
Reconstructing single-cell karyotype alterations in colorectal cancer identifies punctuated and gradual diversification patterns.
Authors
JournalNat Genet
PubMed ID34211178
Permeabilization of Drosophila embryos for introduction of small molecules.
Authors
JournalInsect Biochem Mol Biol
PubMed ID20727969
Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design.