Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
AuthorsGudnason H,Dufva M,Bang DD,Wolff A
JournalNucleic acids research
PubMed ID17897966
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential ... More
Analysis of the distribution of MRI contrast agents in the livers of small animals by means of complementary microscopies.
BACKGROUND: Magnetic resonance imaging (MRI) contrast agents contain magnetic molecules such as iron (Fe) or gadolinium (Gd) that are injected in vivo into rats or mice to study their distribution inside the liver. Fluorescent europium (Eu) can be used as a model of Gd to obtain comparable information of this ... More
Relationships among Bacterial Cell Size, Productivity, and Genetic Diversity in Aquatic Environments using Cell Sorting and Flow Cytometry.
AuthorsBernard L, Courties C, Servais P, Troussellier M, Petit M, Lebaron P
JournalMicrob Ecol
PubMed ID11029083
The study of relationships between cell size and productivity is of key importance in microbial ecology to understand which members of natural aquatic communities are responsible for the overall activity and/or productivity. Flow sorting of microorganisms from different environmental samples was used to analyze the activity of bacterial cells depending ... More
Systematic characterization of nuclear proteome during apoptosis: a quantitative proteomic study by differential extraction and stable isotope labeling.
AuthorsHwang SI, Lundgren DH, Mayya V, Rezaul K, Cowan AE, Eng JK, Han DK
JournalMol Cell Proteomics
PubMed ID16540461
'Identification and characterization of the nuclear proteome is important for detailed understanding of multiple signaling events in eukaryotic cells. Toward this goal, we extensively characterized the nuclear proteome of human T leukemia cells by sequential extraction of nuclear proteins with different physicochemical properties using three buffer conditions. This large scale ... More
Monitoring phytoplankton, bacterioplankton, and virioplankton in a coastal inlet (Bedford Basin) by flow cytometry.
AuthorsLi WK, Dickie PM
JournalCytometry
PubMed ID11429774
'BACKGROUND: To establish the prevailing state of the ecosystem for the assessment of long-term change, the abundance of microbial plankton in Bedford Basin (Nova Scotia, Canada) is monitored weekly by flow cytometry. METHODS: Phytoplankton are detected by their chlorophyll autofluorescence. Those that contain phycoerythrin are designated as Synechococcus cyanobacteria or ... More
Nucleic acid dyes for detection of apoptosis in live cells.
AuthorsFrey T
JournalCytometry
PubMed ID8582249
'Apoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser. The collection of dyes giving this staining pattern includes reagents ... More
In situ activity of suspended and immobilized microbial communities as measured by fluorescence lifetime imaging.
AuthorsWalczysko P, Kuhlicke U, Knappe S, Cordes C, Neu TR,
JournalAppl Environ Microbiol
PubMed ID17981940
'In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fluorescence lifetimes in ... More
Effects of incubation temperature and time after thawing on viability assessment of peripheral hematopoietic progenitor cells cryopreserved for transplantation.
AuthorsYang H, Acker JP, Cabuhat M, McGann LE
JournalBone Marrow Transplant
PubMed ID14595390
'Three widely used viability assessments were compared: (1) membrane integrity of nucleated cells using trypan blue (TB) exclusion and a fluorometric membrane integrity assay (SYTO 13 and propidium iodide), (2) enumeration of viable CD34+ cells, and (3) clonogenic assay (granulocyte-macrophage colony-forming units, CFU-GM). Post thaw peripheral hematopoietic progenitor cells (HPC) ... More
Manual punch versus power harvesting of osteochondral grafts.
AuthorsEvans PJ, Miniaci A, Hurtig MB
JournalArthroscopy
PubMed ID15007320
'PURPOSE: The purpose of the study was to analyze the effect on chondrocyte viability of 2 existing methods of harvesting osteochondral grafts used for articular cartilage resurfacing. TYPE OF STUDY: Acute animal experiment. METHODS: Power (P) trephine versus manual (M) punch harvesting was tested; 2.7-mm and 4.5-mm dowels were harvested ... More
Neocentromere-mediated chromosome movement in maize.
AuthorsYu HG, Hiatt EN, Chan A, Sweeney M, Dawe RK
JournalJ Cell Biol
PubMed ID9362502
'Neocentromere activity is a classic example of nonkinetochore chromosome movement. In maize, neocentromeres are induced by a gene or genes on Abnormal chromosome 10 (Ab10) which causes heterochromatic knobs to move poleward at meiotic anaphase. Here we describe experiments that test how neocentromere activity affects the function of linked centromere/kinetochores ... More
Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria.
AuthorsGasol JM, Zweifel UL, Peters F, Fuhrman JA, Hagström A
JournalAppl Environ Microbiol
PubMed ID10508078
'Total bacterial abundances estimated with different epifluorescence microscopy methods (4'',6-diamidino-2-phenylindole [DAPI], SYBR Green, and Live/Dead) and with flow cytometry (Syto13) showed good correspondence throughout two microcosm experiments with coastal Mediterranean water. In the Syto13-stained samples we could differentiate bacteria with apparent high DNA (HDNA) content and bacteria with apparent low ... More
AuthorsDosogne H, Vangroenweghe F, Mehrzad J, Massart-Leën AM, Burvenich C
JournalJ Dairy Sci
PubMed ID12703619
'Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk. We have developed a flow cytometric differential leukocyte count method for low SCC milk. The procedure consists of 1) 1.5 ml of ... More
Coupling Bacterial Activity Measurements with Cell Sorting by Flow Cytometry.
AuthorsServais P, Courties C, Lebaron P, Troussellier M
JournalMicrob Ecol
PubMed ID10441710
'> Abstract A new procedure to investigate the relationship between bacterial cell size and activity at the cellular level has been developed; it is based on the coupling of radioactive labeling of bacterial cells and cell sorting by flow cytometry after SYTO 13 staining. Before sorting, bacterial cells were incubated ... More
Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems?
AuthorsLebaron P, Servais P, Agogué H, Courties C, Joux F
JournalAppl Environ Microbiol
PubMed ID11282632
'The nucleic acid contents of individual bacterial cells as determined with three different nucleic acid-specific fluorescent dyes (SYBR I, SYBR II, and SYTO 13) and flow cytometry were compared for different seawater samples. Similar fluorescence patterns were observed, and bacteria with high apparent nucleic acid contents (HNA) could be discriminated ... More
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
AuthorsMason DJ, Shanmuganathan S, Mortimer FC, Gant VA
JournalAppl Environ Microbiol
PubMed ID9647848
'The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by ... More
Suppression of Fas/APO-1-mediated apoptosis by mitogen-activated kinase signaling.
AuthorsHolmström TH, Chow SC, Elo I, Coffey ET, Orrenius S, Sistonen L, Eriksson JE
JournalJ Immunol
PubMed ID9510160
'Jurkat T cells undergo rapid apoptosis upon stimulation of the Fas/APO-1 (CD95) receptor. We examined the role of the mitogen-activated protein kinase (MAPK) cascade as a negative regulator of Fas-mediated apoptosis. To this end, we used both physiologic and artificial activators of MAPK, all of which activate MAPK by distinct ... More
Assessment of the effects of gramicidin, formaldehyde, and surfactants on Escherichia coli by flow cytometry using nucleic acid and membrane potential dyes.
AuthorsComas J, Vives-Rego J
JournalCytometry
PubMed ID9298812
'Two membrane potential sensitive dyes (Rhodamine 123 and bis-oxonol) and three nucleic acid dyes (propidium iodide, SYTO-13, and SYTO-17) were used to assess the effect of surfactants on Escherichia coli. The ability of E. coli to be stained by these probes was validated at different physiological states. Propidium iodide was ... More
Identification of an in vivo inhibitor of Bacillus anthracis spore germination.
AuthorsAkoachere M, Squires RC, Nour AM, Angelov L, Brojatsch J, Abel-Santos E
JournalJ Biol Chem
PubMed ID17296608
'Germination of Bacillus anthracis spores into the vegetative form is an essential step in anthrax pathogenicity. This process can be triggered in vitro by the common germinants inosine and alanine. Kinetic analysis of B. anthracis spore germination revealed synergy and a sequential mechanism between inosine and alanine binding to their ... More
Integrating conflicting chemotactic signals. The role of memory in leukocyte navigation.
AuthorsFoxman EF, Kunkel EJ, Butcher EC
JournalJ Cell Biol
PubMed ID10545501
'Leukocytes navigate through complex chemoattractant arrays, and in so doing, they must migrate from one chemoattractant source to another. By evaluating directional persistence and chemotaxis during neutrophil migration under agarose, we show that cells migrating away from a local chemoattractant, against a gradient, display true chemotaxis to distant agonists, often ... More
Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry.
AuthorsPoot M, Gibson LL, Singer VL
JournalCytometry
PubMed ID9098628
'We characterized the ability of six SYTO nucleic acid stains and a mitochondrial stain to resolve by flow cytometry camptothecin-induced apoptotic and non-apoptotic cells. Staining live human lymphoid B-cells showed such resolution with SYTO 11, 12, 13, 14, and 16 dyes. H9, HL-60, and Jurkat cells did not show resolution ... More
Cell death and proliferation in acute slices and organotypic cultures of mammalian CNS.
AuthorsLossi L, Alasia S, Salio C, Merighi A,
JournalProg Neurobiol
PubMed ID19552996
'Analysis of the interplay between cell proliferation and death has been greatly advantaged by the development of CNS slice preparations. In slices, interactions between neurons and neurons and the glial cells are fundamentally preserved in a fashion close to the in vivo situation. In parallel, these preparations offer the possibility ... More
Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
AuthorsLebaron P, Parthuisot N, Catala P
JournalAppl Environ Microbiol
PubMed ID9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More
Flow cytometry counting of CD34+ cells in whole blood.
AuthorsFornas O, Garcia J, Petriz J
JournalNat Med
PubMed ID10888936
Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry.
AuthorsGuindulain T, Comas J, Vives-Rego J
JournalAppl Environ Microbiol
PubMed ID9361447
Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the histograms obtained after DNase and RNase treatments. ... More
SYTO dyes and histoproteins--myriad of applications.
AuthorsTárnok A,
JournalCytometry A
PubMed ID18496838
SYTO dyes differ from each other in one or more characteristics, including cell permeability, ?uorescence enhancement upon binding nucleic acids, excitation and emission spectra, DNA/RNA selectivity and binding af?nity. This makes them versatile tools for cell biologists. ... More
Optical sensors for detection of bacteria. 1. General concepts and initial development.
AuthorsChuang H, Macuch P, Tabacco MB
JournalAnal Chem
PubMed ID11217747
The concept of using immobilized nucleic acid stains as detection chemistry to fabricate optical bacterial sensors is first demonstrated. SYTO 13 (a green fluorescent cell stain) is used as the molecular recognition element and fluorescent reporter in the sensor. The sensor responds to aqueous and aerosolized bacterial samples in 15 ... More
Flow cytometric analysis of a marine LAS-degrading consortia.
The specific nucleic acid fluorochrome SYTO-13 was used in flow cytometric analysis to assess changes in the density and heterogeneity of marine bacterial populations which biodegrade linear alkylbenzene sulphonate (LAS). Seawater samples with LAS and incubated in the laboratory (20 degrees C, 100 rpm, 30 days) were used to monitor ... More
Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems.
AuthorsUllal AJ, Pisetsky DS, Reich CF,
JournalCytometry A
PubMed ID20104574
Microparticles (MPs) are small membrane-bound vesicles that are released from activated or dying cells by a blebbing process. These particles contain nuclear and cytoplasmic components and represent unique biomarkers for disease. The small size of particles, however, limits detection using flow cytometry with either light scatter or staining for surface ... More
Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms.
AuthorsNeu TR, Kuhlicke U, Lawrence JR
JournalAppl Environ Microbiol
PubMed ID11823234
A major limitation for the use of two-proton laser scanning microscopy (2P-LSM) in biofilm and other studies is the lack of a thorough understanding of the excitation-emission responses of potential fluorochromes. In order to use 2P-LSM, the utility of various fluorochromes and probes specific for a range of biofilm constituents ... More
Glutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial function.
AuthorsAnkarcrona M, Dypbukt JM, Bonfoco E, Zhivotovsky B, Orrenius S, Lipton SA, Nicotera P
JournalNeuron
PubMed ID7576644
During ischemic brain injury, glutamate accumulation leads to overstimulation of postsynaptic glutamate receptors with intracellular Ca2+ overload and neuronal cell death. Here we show that glutamate can induce either early necrosis or delayed apoptosis in cultures of cerebellar granule cells. During and shortly after exposure to glutamate, a subpopulation of ... More
Quantification of neurotoxicity and identification of cellular subsets in a three-dimensional brain model.
AuthorsPulliam L, Stubblebine M, Hyun W
JournalCytometry
PubMed ID9581626
Imaging of cells in a large intact three-dimensional tissue remains difficult. Quantification and identification of cell damage in a mixed culture system has been limited by the inability of fluorescent probes to discriminate types of cellular death and penetrate tissue more that 100 microm thick. We have investigated several probes ... More
An immunohistochemical method for the detection of proteins in the vertebrate lens.
AuthorsReed NA, Oh DJ, Czymmek KJ, Duncan MK
JournalJ Immunol Methods
PubMed ID11384685
Fluorescence immunohistochemistry has traditionally been difficult or impossible to perform on the vertebrate lens because of its extremely high protein content. Described here is a robust and rapid method for preparing and labeling vertebrate eyes for confocal microscopy. This technique has successfully been applied to localize proteins in the lens ... More
Cytoskeletal breakdown and apoptosis elicited by NO donors in cerebellar granule cells require NMDA receptor activation.
AuthorsBonfoco E, Leist M, Zhivotovsky B, Orrenius S, Lipton SA, Nicotera P
JournalJ Neurochem
PubMed ID8931482
We have recently demonstrated that nitric oxide (NO) donors can trigger either apoptosis or necrosis of neurons as a function of the intensity of the exposure. Here, we show that the apoptosis induced by the NO donors S-nitrosocysteine (SNOC) or S-nitroso-N-acetyl-penicillamine (SNAP) in cultured cerebellar granule cells (CGCs) depends on ... More
Flow cytometry assessment of bacterioplankton in tropical marine environments.
AuthorsAndrade L, Gonzalez AM, Araujo FV, Paranhos R
JournalJ Microbiol Methods
PubMed ID14607430
Flow cytometry was used to characterize bacterioplankton from two tropical environments in Brazil: the eutrophic Guanabara Bay and the oligotrophic southwest Atlantic Ocean. Bacterial abundance was evaluated by flow cytometry, and cells were stained with SYTO 13, allowing demonstration of differences in nucleic acid content. Bacterial production was also evaluated ... More
Discrimination of DNA and RNA in cells by a vital fluorescent probe: lifetime imaging of SYTO13 in healthy and apoptotic cells.
Authorsvan Zandvoort MA, de Grauw CJ, Gerritsen HC, Broers JL, oude Egbrink MG, Ramaekers FC, Slaaf DW
JournalCytometry
PubMed ID11933012
BACKGROUND: Of the few vital DNA and RNA probes, the SYTO dyes are the most specific for nucleic acids. However, they show no spectral contrast upon DNA or RNA binding. We show that fluorescence lifetime imaging using two-photon excitation of SYTO13 allows differential and simultaneous imaging of DNA and RNA ... More
A multicolor, no-lyse no-wash assay for the absolute counting of CD34+ cells by flow cytometry.
AuthorsAlvarez-Larran A, Jover L, Marin P, Petriz J
JournalCytometry
PubMed ID12360574
BACKGROUND: We previously developed a method for counting CD34(+) cells in unlysed whole blood. This method was applied to normal human bone marrow, peripheral blood after mobilization of progenitor cells, leukapheresis products, and cord blood and was validated with two different lyse-no wash methods. However, the main advantage that we ... More
Kinetics and mechanism of intercellular ice propagation in a micropatterned tissue construct.
AuthorsIrimia D, Karlsson JO
JournalBiophys J
PubMed ID11916845
Understanding the effects of cell-cell interaction on intracellular ice formation (IIF) is required to design optimized protocols for cryopreservation of tissue. To determine the effects of cell-cell interactions during tissue freezing, without confounding effects from uncontrolled factors (such as time in culture, cell geometry, and cell-substrate interactions), HepG2 cells were ... More
Involvement of RNA and DNA in the staining of Escherichia coli by SYTO 13.
AuthorsGuindulain T, Vives-Rego J
JournalLett Appl Microbiol
PubMed ID11874539
AIMS: To assess the extent to which DNA and RNA bacterial content contributes to fluorescent response of SYTO 13. METHODS AND RESULTS: RNA and DNA of Escherichia coli 536 cells were extracted and fluorimetrically quantified to compare the different contents, throughout a 24 h culture, with their SYTO 13 fluorescence ... More
Arrays of self-assembled monolayers for studying inhibition of bacterial adhesion.
This paper describes a simple and convenient method for the rapid screening of potential inhibitors of bacterial adhesion and for the quantitative evaluation of the efficacy of the inhibitors using arrays of self-assembled monolayers (SAMs) of alkanethiolates on gold that are presented on a 96-well microtiter plate. The SAMs present ... More
Nuclear and cytosolic calcium changes in osteoclasts stimulated with ATP and integrin-binding peptide.
AuthorsParkinson N, Bolsover S, Mason W
JournalCell Calcium
PubMed ID9883275
Cytosolic calcium modulates the activity of osteoclasts, large multinucleate cells that resorb bone. Nuclear events, such as gene transcription, are also calcium-regulated in these cells, and fluorescence imaging has suggested that calcium signals produced by some stimuli are specifically targeted to, or amplified within, osteoclast nuclei. We used two alternative ... More
Intercellular ice propagation: experimental evidence for ice growth through membrane pores.
AuthorsAcker JP, Elliott JA, McGann LE
JournalBiophys J
PubMed ID11509353
Propagation of intracellular ice between cells significantly increases the prevalence of intracellular ice in confluent monolayers and tissues. It has been proposed that gap junctions facilitate ice propagation between cells. This study develops an equation for capillary freezing-point depression to determine the effect of temperature on the equilibrium radius of ... More
Two-photon microscopy of vital murine elastic and muscular arteries. Combined structural and functional imaging with subcellular resolution.
AuthorsMegens RT, Reitsma S, Schiffers PH, Hilgers RH, De Mey JG, Slaaf DW, oude Egbrink MG, van Zandvoort MA
JournalJ Vasc Res
PubMed ID17192719
Understanding vascular pathologies requires insight in the structure and function, and, hence, an imaging technique combining subcellular resolution, large penetration depth, and optical sectioning. We evaluated the applicability of two-photon laser-scanning microscopy (TPLSM) in large elastic and small muscular arteries under physiological conditions. Elastic (carotid) and muscular (uterine, mesenteric) arteries ... More
Cellular effects of monohydrochloride of L-arginine, N-lauroyl ethylester (LAE) on exposure to Salmonella typhimurium and Staphylococcus aureus.
AuthorsRodríguez E, Seguer J, Rocabayera X, Manresa A
JournalJ Appl Microbiol
PubMed ID15078505
AIMS: Here we study the effect of monohydrochloride of L-arginine, N(alpha)-lauroyl ethylester (LAE), a cationic preservative derived from lauric acid and arginine, on the cell envelopes of Salmonella typhimurium and Staphylococcus aureus at sub-lethal concentration such as their respective minimal inhibitory concentrations, 32 and 8 microg ml(-1), respectively. METHODS AND ... More
Determination of in situ bacterial growth rates in aquifers and aquifer sediments.
AuthorsMailloux BJ, Fuller ME
JournalAppl Environ Microbiol
PubMed ID12839747
Laboratory and field-scale studies with stained cells were performed to monitor cell growth in groundwater systems. During cell division, the fluorescence intensity of the protein stain 5-(and 6-)-carboxyfluorescein diacetate succinimidyl ester (CFDA/SE) for each cell is halved, and the intensity can be tracked with a flow cytometer. Two strains of ... More
A double-label technique that monitors sulfur mustard damage to nuclei and mitochondria of normal human epidermal keratinocytes in vitro.
AuthorsCook JR, Van Buskirk RG
JournalToxicol Pathol
PubMed ID9323838
Sulfur mustard and 2-chloro ethyl ethyl sulfide (CEES, a sulfur mustard analog) is known to have immediate (minutes), long-term (hours to days), and toxic effects on human skin. Research was directed toward developing a single in vitro assay that might reflect both these short-term and long-term effects of this vesicating ... More
Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry.
AuthorsCui HH, Valdez JG, Steinkamp JA, Crissman HA
JournalCytometry A
PubMed ID12596251
BACKGROUND: Simultaneous measurement of cellular DNA and RNA content provides information for determination of the functional status of cells and, clinically, for the diagnosis and grading assessment of various tumors. Most current flow cytometric methods are based on resolving the fluorescence emission spectra of dyes that bind preferentially to either ... More
Highly sensitive and specific detection of P-glycoprotein function for haematological and solid tumour cells using a novel nucleic acid stain.
AuthorsBroxterman HJ, Schuurhuis GJ, Lankelma J, Oberink JW, Eekman CA, Claessen AM, Hoekman K, Poot M, Pinedo HM
JournalBr J Cancer
PubMed ID9376262
Progress in our understanding of the contribution of P-glycoprotein (P-gp)-mediated resistance to chemotherapy failure in haematological as well as solid tumours has been hampered by the lack of highly sensitive, reliable methods for the detection of P-gp function in fresh human tumour cells. The present study identifies the novel nucleic ... More
Options of flow cytometric three-colour DNA measurements to quantitate EGFR in subpopulations of human bladder cancer.
AuthorsBrockhoff G, Endl E, Minuth W, Hofstädter F, Knüchel R
JournalAnal Cell Pathol
PubMed ID8844105
Flow cytometric multi-parameter analysis has proven to be a powerful tool to characterize subpopulations of cell suspensions, and is applied routinely in hematology. However, in studies of cancer where there is interest in defining phenotypic markers in conjunction with DNA content, this method has hardly been applied [6]. Our objective ... More
Overexpression of PAX6(5a) in lens fiber cells results in cataract and upregulation of (alpha)5(beta)1 integrin expression.
AuthorsDuncan MK, Kozmik Z, Cveklova K, Piatigorsky J, Cvekl A
JournalJ Cell Sci
PubMed ID10954416
The PAX6 gene, a key regulator of eye development, produces two major proteins that differ in paired domain structure: PAX6 and PAX6(5a). It is known that an increase in the PAX6(5a) to PAX6 ratio leads to multiple ocular defects in humans. Here, transgenic mice were created that overexpress human PAX6(5a) ... More
Variation in human islet viability based on different membrane integrity stains.
AuthorsBarnett MJ, McGhee-Wilson D, Shapiro AM, Lakey JR
JournalCell Transplant
PubMed ID15565860
Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability staining of ... More
Cytometric monitoring of growth, sporogenesis and spore cell sorting in Paenibacillus polymyxa (formerly Bacillus polymyxa).
AuthorsComas-Riu J, Vives-Rego J
JournalJ Appl Microbiol
PubMed ID11872123
AIMS: Formation of bacterial endospores is a basic process in Gram-positive bacteria and has implications for health, industry and the environment. Flow cytometry offers a practical alternative for the rapid detection, enumeration and characterization of bacterial endospores. METHODS AND RESULTS: Paenibacillus polymyxa was chosen for this study because its spores ... More
Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk.
AuthorsGunasekera TS, Veal DA, Attfield PV
JournalInt J Food Microbiol
PubMed ID12878385
Monitoring the quality and safety of milk requires careful analysis of microbial and somatic cell loading. Our aim was to demonstrate proof of the principle that flow cytometry (FCM), coupled with fluorescence techniques for distinguishing between cell types, could potentially be employed in a wide variety of biological assays relevant ... More
Characterization and expression of calpain 10. A novel ubiquitous calpain with nuclear localization.
AuthorsMa H, Fukiage C, Kim YH, Duncan MK, Reed NA, Shih M, Azuma M, Shearer TR
JournalJ Biol Chem
PubMed ID11375982
Calpains are calcium-dependent intracellular nonlysosomal proteases that are believed to hydrolyze specific substrates important in calcium-regulated signaling pathways. Recently, an atypical member of the calpain family, calpain 10, was described, and genetic variation in this gene was associated with an increased risk of type II diabetes mellitus in humans. In ... More
Autologous Adipose Tissue-Derived Mesenchymal Stem Cells Introduced by Biliary Stents or Local Immersion in Porcine Bile Duct Anastomoses.
AuthorsZhang Y, Sharma A, Joo DJ, Nelson E, AbuRmilah A, Amiot BP, Boyer CJ, Alexander JS, Jalan-Sakrikar N, Martin J, Moreira R, Chowdhury SA, Smart M, Dietz AB, Nyberg SL, Heimbach JK, Huebert RC
JournalLiver Transpl
PubMed ID31742878
'Biliary complications (strictures and leaks) represent major limitations in living donor liver transplantation. Mesenchymal stem cells (MSCs) are a promising modality to prevent biliary complications because of immunosuppressive and angiogenic properties. Our goal was to evaluate the safety of adipose-derived MSC delivery to biliary anastomoses in a porcine model. Secondary ... More
Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes.
AuthorsMobarrez F, Fuzzi E, Gunnarsson I, Larsson A, Eketjäll S, Pisetsky DS, Svenungsson E
JournalJ Autoimmun
PubMed ID31103269
'Microparticles (MPs) are small extracellular vesicles released from apoptotic or activated cells through a blebbing process. MPs express surface molecules from their parental cells and they bind IgG to form circulating immune complexes (MP-ICs) in patients with systemic lupus erythematosus (SLE). Through investigation of MP size, IgG expression, content of ... More
DNA threads released by activated CD4
AuthorsCostanza M, Poliani PL, Portararo P, Cappetti B, Musio S, Pagani F, Steinman L, Colombo MP, Pedotti R, Sangaletti S
JournalProc Natl Acad Sci U S A
PubMed ID30988194
'The extrusion of DNA traps contributes to a key mechanism in which innate immune cells clear pathogens or induce sterile inflammation. Here we provide evidence that CD4' ... More
Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics.
Authors
JournalNat Protoc
PubMed ID24722403
Neutrophils Culture in Collagen Gel System.
Authors
JournalFront Immunol
PubMed ID35140721
The spatial landscape of lung pathology during COVID-19 progression.
Authors
JournalNature
PubMed ID33780969
Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions.
Authors
JournalNat Commun
PubMed ID33712587
Shore crabs reveal novel evolutionary attributes of the mushroom body.
Persistent Mycobacterium tuberculosis infection in mice requires PerM for successful cell division.
Authors
JournalElife
PubMed ID31751212
Mushroom body evolution demonstrates homology and divergence across Pancrustacea.
Authors
JournalElife
PubMed ID32124731
Deletion of Topoisomerase 1 in excitatory neurons causes genomic instability and early onset neurodegeneration.
Authors
JournalNat Commun
PubMed ID32327659
14-Helical β-Peptides Elicit Toxicity against C. albicans by Forming Pores in the Cell Membrane and Subsequently Disrupting Intracellular Organelles.
Authors
JournalCell Chem Biol
PubMed ID30581136
Rhizobium tropici CIAT 899 copA gene plays a fundamental role in copper tolerance in both free life and symbiosis with Phaseolus vulgaris.
AuthorsElizalde-Díaz JP, Hernández-Lucas I, Medina-Aparicio L, Dávalos A, Leija A, Alvarado-Affantranger X, García-García JD, Hernández G, Garcia-de Los Santos A
JournalMicrobiology
PubMed ID31081746
Rhizobium tropici CIAT 899 is a facultative symbiotic diazotroph able to deal with stressful concentrations of metals. Nevertheless the molecular mechanisms involved in metal tolerance have not been elucidated. Copper (Cu ... More
A High-Sensitivity 10-Color Flow Cytometric Minimal Residual Disease Assay in B-Lymphoblastic Leukemia/Lymphoma Can Easily Achieve the Sensitivity of 2-in-10
AuthorsTembhare PR, Subramanian Pg PG, Ghogale S, Chatterjee G, Patkar NV, Gupta A, Shukla R, Badrinath Y, Deshpande N, Narula G, Rodrigues P, Girase K, Dhaliwal D, Prasad M, Shetty D, Banavali S, Gujral S
JournalCytometry B Clin Cytom
PubMed ID31197916
Flow-cytometric minimal residual disease (FC-MRD) monitoring is a well-established risk-stratification factor in B-lymphoblastic leukemia/lymphoma (-B-ALL) and is being considered as a basis for deintensification or escalation in treatment protocols. However, currently practiced standard FC-MRD has limited sensitivity (up to 0.01%) and higher false MRD-negative rate. Hence, a highly sensitive, widely ... More