Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.
AuthorsYing W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA
JournalJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
PubMed ID10698074
Nitric oxide reversibly inhibits the migration of cultured vascular smooth muscle cells.
AuthorsSarkar R, Meinberg EG, Stanley JC, Gordon D, Webb RC
JournalCirc Res
PubMed ID8575065
'Augmentation of nitric oxide (NO) production in vivo decreases lesions in a variety of models of arterial injury, and inhibition of NO synthase exacerbates experimental intimal lesions. Both vascular smooth muscle cell (VSMC) proliferation and migration contribute to lesion formation. Although NO inhibits VSMC proliferation, its effects on VSMC migration ... More
Superoxide formation and macrophage resistance to nitric oxide-mediated apoptosis.
'RAW 264.7 macrophages, when challenged with a combination of lipopolysaccharide (10 microg/ml) and interferon-gamma (100 units/ml), respond with endogenous NO. formation, which ultimately results in apoptotic cell death. Apoptosis is detected morphologically by chromatin condensation. Concomitantly we noticed the accumulation of the tumor suppressor protein p53. NO.-derived apoptosis was blocked ... More
Nitric oxide inhibits numerous features of mast cell-induced inflammation.
AuthorsGaboury JP, Niu XF, Kubes P
JournalCirculation
PubMed ID8548905
'BACKGROUND. We previously reported that mast cell degranulation causes histamine and P-selectin-dependent leukocyte rolling and platelet-activating factor (PAF)- and CD18-associated leukocyte adhesion, whereas others have reported serotonin-induced edema formation. The purpose of the present study was to determine whether nitric oxide (NO) could inhibit the mast cell-induced multistep recruitment of ... More
Expression of inducible nitric oxide synthase in cerebral endothelial cells is regulated by cytokine-activated astrocytes.
AuthorsBorgerding RA, Murphy S
JournalJ Neurochem
PubMed ID7543934
'A nitric oxide (NO) synthase (NOS) can be induced in both astrocytes and cerebral endothelial cells with a combination of interleukin-1 beta/interferon-gamma or lipopolysaccharide/interferon-gamma, respectively. Exogenous NO, either from the chemical donor spermine NONOate or from activated astrocytes, affected the expression of inducible NOS in cerebral endothelial cells. In cerebral ... More
Effects of nitric oxide on P2Y receptor resensitization in spontaneously hypertensive rat mesangial cells.
AuthorsLiu R, Persson AE
JournalJ Hypertens
PubMed ID12195127
'BACKGROUND: Cellular responses to agonists of G protein-coupled receptors are usually rapidly attenuated - a process known as ''receptor desensitization''. The mechanisms that attenuate signalling are important both physiologically and therapeutically. OBJECTIVE: To evaluate the effects of nitric oxide on the P2Y receptor resensitization in cultured glomerular mesangial cells in ... More
Bcl-2 protects macrophages from nitric oxide-induced apoptosis.
AuthorsMessmer UK, Reed UK, Brüne B
JournalJ Biol Chem
PubMed ID8702745
'Endogenously generated or exogenously supplied nitric oxide (NO)-induced apoptotic cell death in the mouse macrophage cell line RAW 264.7. Apoptotic signaling caused an early accumulation of the tumor suppressor p53 prior to DNA fragmentation. Contrary to the notion of specific activating signals, inhibitory transduction mechanisms largely remain unknown. Therefore, RAW ... More
Endogenous nitric oxide inhibits chloride transport in the thick ascending limb.
AuthorsPlato CF, Stoos BA, Wang D, Garvin JL
JournalAm J Physiol
PubMed ID9887091
'Nitric oxide (NO) inhibits transport in various nephron segments, and the thick ascending limb of the loop of Henle (TALH) expresses NO synthase (NOS). However, the effects of NO on TALH transport have not been extensively studied. We hypothesized that endogenously produced NO directly decreases NaCl transport by the TALH. ... More
Determination of nitric oxide using fluorescence spectroscopy.
AuthorsMiles AM, Wink DA, Cook JC, Grisham MB
JournalMethods Enzymol
PubMed ID8782577
Nitric oxide donor compounds inhibit the toxicity of oxidized low-density lipoprotein to endothelial cells.
AuthorsStruck AT, Hogg N, Thomas JP, Kalyanaraman B
JournalFEBS Lett
PubMed ID7698340
Photo-oxidized low-density lipoprotein is cytotoxic to bovine aortic endothelial cells in a concentration-dependent manner. Total cell killing occurs at a concentration of 600 mumol/l lipid hydroperoxide (LOOH). Selenium deficiency enhances the toxicity of LOOH such that 300 mumol/l LOOH is cytotoxic. This toxicity is inhibited by desferrioxamine, a transition metal ... More
Nitric oxide-inducible expression of heme oxygenase-1 in human cells. Translation-independent stabilization of the mRNA and evidence for direct action of nitric oxide.
AuthorsBouton C, Demple B
JournalJ Biol Chem
PubMed ID11032845
Expression of heme oxygenase-1 (HO-1) in mammalian cells contributes to resistance to various types of free radical damage. Nitric oxide (NO) induces HO-1 in many cell types, but the specific contribution of transcriptional or post-transcriptional effects to this induction have remained unresolved. Here we show that the extent of HO-1 ... More
Heme and the endothelium. Effects of nitric oxide on catalytic iron and heme degradation by heme oxygenase.
AuthorsJuckett M, Zheng Y, Yuan H, Pastor T, Antholine W, Weber M, Vercellotti G
JournalJ Biol Chem
PubMed ID9722574
We studied the effects of nitric oxide (NO) on the control of excess cellular heme and release of catalytically active iron. Endothelial cells (ECs) exposed to hemin followed by a NO donor have a ferritin content that is 16% that of cells exposed to hemin alone. Hemin-treated ECs experience a ... More
Nitric oxide protects against alkyl peroxide-mediated cytotoxicity: further insights into the role nitric oxide plays in oxidative stress.
AuthorsWink DA, Cook JA, Krishna MC, Hanbauer I, DeGraff W, Gamson J, Mitchell JB
JournalArch Biochem Biophys
PubMed ID7786021
Endogenously formed nitric oxide (NO) possesses diverse properties such as regulating physiological functions, exerting specific toxic effects, and protecting against various toxic substances. Recent studies suggest that in the presence of reactive oxygen species, NO can serve as an antioxidant. We show here that NO delivered from the NO donor ... More
Modulation of iron regulatory protein functions. Further insights into the role of nitrogen- and oxygen-derived reactive species.
AuthorsBouton C, Raveau M, Drapier JC
JournalJ Biol Chem
PubMed ID8567693
Iron regulatory protein (IRP) is a cytosolic bifunctional [Fe-S] protein which exhibits aconitase activity or binds iron responsive elements (IREs) in untranslated regions of specific mRNA. The modulators of these activities are the intracellular concentration of iron and, as recently described, NO synthase activity. In this study, we attempted to ... More
Nitric oxide/nucleophile complexes inhibit the in vitro proliferation of A375 melanoma cells via nitric oxide release.
AuthorsMaragos CM, Wang JM, Hrabie JA, Oppenheim JJ, Keefer LK
JournalCancer Res
PubMed ID8425188
Cell-mediated antitumor effects have, in part, been attributed to the production of NO. Compounds which generate NO might, therefore, be useful in attenuating the growth of tumor cells. Six nitric oxide/nucleophile adducts that release NO spontaneously in solution were tested for their effectiveness in inhibiting DNA synthesis in A375 human ... More
Nitric oxide inhibition of endothelial cell mitogenesis and proliferation.
AuthorsSarkar R, Webb RC, Stanley JC
JournalSurgery
PubMed ID7638744
BACKGROUND. Endothelial cell (EC) proliferation is essential in vascular repair after injury to the vessel wall. Impaired EC proliferation may be an important factor contributing to vessel wall disease. Nitric oxide (NO) inhibits proliferation of many cells, including smooth muscle cells (SMC). We tested the hypothesis that NO inhibits EC ... More
Superoxide reacts with nitric oxide to nitrate tyrosine at physiological pH via peroxynitrite.
AuthorsReiter CD, Teng RJ, Beckman JS
JournalJ Biol Chem
PubMed ID10906340
Tyrosine nitration is a widely used marker of peroxynitrite (ONOO(-)) produced from the reaction of nitric oxide with superoxide. Pfeiffer and Mayer (Pfeiffer, S., and Mayer, B. (1998) J. Biol. Chem. 273, 27280-27285) reported that superoxide produced from hypoxanthine plus xanthine oxidase in combination with nitric oxide produced from spermine ... More
Tetrahydrobiopterin inhibits monomerization and is consumed during catalysis in neuronal NO synthase.
AuthorsReif A, Fröhlich LG, Kotsonis P, Frey A, Bömmel HM, Wink DA, Pfleiderer W, Schmidt HH
JournalJ Biol Chem
PubMed ID10455167
The biosynthesis of nitric oxide (NO) is catalyzed by homodimeric NO synthases (NOS). For unknown reasons, all NOS co-purify with substoichiometric amounts of (6R)-5,6,7,8-tetrahydrobiopterin (H(4)Bip) and require additional H(4)Bip for maximal activity. We examined the effects of H(4)Bip and pterin-derived inhibitors (anti-pterins) on purified neuronal NOS-I quaternary structure and H(4)Bip ... More
Formation of S-nitrosothiols via direct nucleophilic nitrosation of thiols by peroxynitrite with elimination of hydrogen peroxide.
Authorsvan der Vliet A, Hoen PA, Wong PS, Bast A, Cross CE
JournalJ Biol Chem
PubMed ID9804785
Peroxynitrite (ONOO-), a potent oxidant formed by reaction of nitric oxide (NO.) with superoxide anion, can activate guanylyl cyclase and is able to induce vasodilation or inhibit platelet aggregation and leukocyte adhesion, via thiol-dependent formation of NO. Reaction of ONOO- with thiols is thought to proceed through formation of a ... More
Low toxicity of nitric oxide against endothelial cells under physiological oxygen partial pressures.
AuthorsIoannidis I, Bätz M, Kirsch M, Korth HG, Sustmann R, De Groot H
JournalBiochem J
PubMed ID9425128
Cultured rat liver endothelial cells were incubated with 1 and 2 mM spermineNONOate at different O2 concentrations in the incubation atmosphere. (Z)-1-{N-[3-Aminopropyl]-N-[4-(3-aminopropylammonio) butyl]-amino}diazen-1-ium-1,2- diolate (spermineNONOate), at 2 mM, was highly cytotoxic at 21% and 95% O2 (as measured by lactate dehydrogenase release); more than 80% of the cells were damaged ... More
Complexes of .NO with nucleophiles as agents for the controlled biological release of nitric oxide. Vasorelaxant effects.
AuthorsMaragos CM, Morley D, Wink DA, Dunams TM, Saavedra JE, Hoffman A, Bove AA, Isaac L, Hrabie JA, Keefer LK
JournalJ Med Chem
PubMed ID1956043
Selected nucleophile/nitric oxide adducts [compounds which contain the anionic moiety, XN(O-)N = O] were studied for their ability to release nitric oxide spontaneously in aqueous solution and for possible vasoactivity. The diversity of structures chosen included those in which the nucleophile residue, X, was that of a secondary amine [Et2N, ... More
Reaction of superoxide and nitric oxide with peroxynitrite. Implications for peroxynitrite-mediated oxidation reactions in vivo.
Peroxynitrite (ONOO(-)/ONOOH), the product of the diffusion-limited reaction of nitric oxide (*NO) with superoxide (O(-*)(2)), has been implicated as an important mediator of tissue injury during conditions associated with enhanced *NO and O(-*)(2) production. Although several groups of investigators have demonstrated substantial oxidizing and cytotoxic activities of chemically synthesized peroxynitrite, ... More
Isolation and characterization of diazoate intermediate upon nitrous acid and nitric oxide treatment of 2'-deoxycytidine.
AuthorsSuzuki T, Nakamura T, Yamada M, Ide H, Kanaori K, Tajima K, Morii T, Makino K
JournalBiochemistry
PubMed ID10353825
The intermediate produced from dCyd by HNO2 and NO treatments was isolated and characterized. When 10 mM dCyd was treated with 100 mM NaNO2 in 1.0 M acetate buffer (pH 3.7) at 37 degrees C, a previously unidentified product was formed. By spectrometric measurements, the product was identified as a ... More
NO decreases thick ascending limb chloride absorption by reducing Na(+)-K(+)-2Cl(-) cotransporter activity.
AuthorsOrtiz PA, Hong NJ, Garvin JL
JournalAm J Physiol Renal Physiol
PubMed ID11592939
We have reported that nitric oxide (NO) inhibits thick ascending limb (THAL) chloride absorption (J(Cl(-))). NaCl transport in the THAL depends on apical Na(+)-K(+)-2Cl(-) cotransporters, apical K(+) channels, and basolateral Na(+)-K(+)-ATPase. However, the transporter inhibited by NO is unknown. We hypothesized that NO decreases THAL J(Cl(-)) by inhibiting the Na(+)-K(+)-2Cl(-) ... More
Nitric oxide generators produce accumulation of chelatable zinc in hippocampal neuronal perikarya.
AuthorsCuajungco MP, Lees GJ
JournalBrain Res
PubMed ID9666098
While zinc is essential for health, it has also been implicated in the neuropathology of several disease states such as Alzheimer's disease, epilepsy and cerebral ischemia. Recent studies have shown that oxidative and nitrosylative stresses can liberate zinc from metalloproteins in vitro. Thus, nitric oxide (NO.), a radical molecule which ... More
Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general mechanism.
AuthorsKirsch M, Lomonosova EE, Korth HG, Sustmann R, de Groot H
JournalJ Biol Chem
PubMed ID9582295
Organic amine-based buffer compounds such as HEPES (Good's buffers) are commonly applied in experimental systems, including those where the biological effects of peroxynitrite are studied. In such studies 3-morpholinosydnonimine N-ethylcarbamide (SIN-1), a compound that simultaneously releases nitric oxide (.NO) and superoxide (O-2), is often used as a source for peroxynitrite. ... More
Modulation of superoxide-dependent oxidation and hydroxylation reactions by nitric oxide.
The rapid and spontaneous interaction between superoxide (O2-.) and nitric oxide (NO) to yield the potent oxidants peroxynitrite (ONOO-) and peroxynitrous acid (ONOOH), has been suggested to represent an important pathway by which tissue may be injured during inflammation. Although several groups of investigators have demonstrated substantial oxidizing and cytotoxic ... More
The nitric oxide/superoxide assay. Insights into the biological chemistry of the NO/O-2. interaction.
AuthorsKelm M, Dahmann R, Wink D, Feelisch M
JournalJ Biol Chem
PubMed ID9092531
Nitric oxide (NO) is a widespread signaling molecule involved in the regulation of an impressive spectrum of diverse cellular functions. Superoxide anions (O-2) not only contribute to the localization of NO action by rapid inactivation, but also give rise to the formation of the potentially toxic species peroxynitrite (ONOO-) and ... More
Anandamide uptake by human endothelial cells and its regulation by nitric oxide.
AuthorsMaccarrone M, Bari M, Lorenzon T, Bisogno T, Di Marzo V, Finazzi-Agrò A
JournalJ Biol Chem
PubMed ID10788462
Anandamide (AEA) has vasodilator activity, which can be terminated by cellular re-uptake and degradation. Here we investigated the presence and regulation of the AEA transporter in human umbelical vein endothelial cells (HUVECs). HUVECs take up AEA by facilitated transport (apparent K(m) = 190 +/- 10 nm and V(max) = 45 ... More
Presentation of nitric oxide regulates monocyte survival through effects on caspase-9 and caspase-3 activation.
AuthorsZeigler MM, Doseff AI, Galloway MF, Opalek JM, Nowicki PT, Zweier JL, Sen CK, Marsh CB
JournalJ Biol Chem
PubMed ID12566444
In the absence of survival factors, blood monocytes undergo spontaneous apoptosis, which involves the activation of caspase-3. Although nitric oxide can block caspase-3 activation and promote cell survival, it can also induce apoptosis. We hypothesized that nitrosothiols that promote protein S-nitrosylation would reduce caspase-3 activation and cell survival, whereas nitric ... More
Fluorescence determination of nitric oxide production in stimulated and activated platelets.
AuthorsKu CJ, Karunarathne W, Kenyon S, Root P, Spence D
JournalAnal Chem
PubMed ID17288406
Nitric oxide (NO) is quantitatively determined in platelets prior to, and after, stimulation with adenosine triphosphate (ATP) or activation with adenosine diphosphate (ADP). Platelets obtained from the whole blood of rabbits were loaded with the fluorescence probe diaminodifluorofluorescein diacetate (DAF-FM DA), and the subsequent NO production was measured as a ... More
Lack of tyrosine nitration by peroxynitrite generated at physiological pH.
AuthorsPfeiffer S, Mayer B
JournalJ Biol Chem
PubMed ID9765252
Nitration of tyrosine residues of proteins has been suggested as a marker of peroxynitrite-mediated tissue injury in inflammatory conditions. The nitration reaction has been extensively studied in vitro by bolus addition of authentic peroxynitrite, an experimental approach hardly reflecting in vivo situations in which the occurrence of peroxynitrite is thought ... More