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链霉亲和素-辣根过氧化物酶 (HRP) 偶联物

链霉亲和素-辣根过氧化物酶 (HRP) 偶联物
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货号数量
SA1000150 μL
货号 SA10001
价格(CNY)
3,999.00
Each
数量:
50 μL
价格(CNY)
3,999.00
Each

Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy. The amount of conjugate provided is sufficient for 25 western blots using 10 ml of working solution per reaction or 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

链霉亲和素-HRP 偶联物通常用于生物素化的蛋白质的免疫检测。该偶联物适用于免疫印迹、ELISA、免疫显微检测。其中所含的偶联物量足以进行25次免疫印迹分析(每次反应使用10 ml 工作溶液)或25次免疫染色反应(每次反应使用1 ml 工作溶液)。
仅供科研使用。不可用于诊断程序。
规格
最大浓度0.75-1.2 mg/mL
标签或染料HRP(辣根过氧化物酶)
产品类型链霉素亲和素偶联物(荧光)
数量50 μL
偶联物HRP
形式液体
产品线Invitrogen
Unit SizeEach
内容与储存
储存在冰箱 (2—8°C) 中。

常见问题解答 (FAQ)

Should I block my western blot membrane before incubating with Streptavidin-Horseradish Peroxidase (HRP) Conjugate (Cat. No. SA10001)?

Yes, you should block the western blot membrane before incubating with Streptavidin-HRP Conjugate. Blocking is essential to reduce non-specific binding and background signal.
We recommend incubating the membrane in 10 mL blocking solution. Gently agitate using a rocker platform for 1 hour at room temperature and then proceed to washing the membrane (Step 5 of the protocol on page 5 of the user manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/streptavidin_conjugates_man.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.