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Plate Frame for 24-well Piko PCR Plates, white - FAQs

查看更多产品信息 Plate Frame for 24-well Piko PCR Plates, white - FAQs (SFR0241)

6 个常见问题解答

Are ultra thin wall (UTW) vessels compatible with real-time experiments?

Yes. All of the UTW plastics we provide are well suited for qPCR reactions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Will ultra thin wall (UTW) vessels automatically speed up reactions on my conventional thermal cycler?

To realize the time savings of the UTW plastics on conventional thermal cyclers, optimization of the protocol is required. This typically involves reducing hold times and changing the actual hold temperatures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Are ultra thin wall (UTW) vessels prone to break more often because of their thinner walls?

When used as instructed, these vessels are remarkably robust and can handle typical laboratory conditions, without fracturing or resulting in loss of volume. It is recommended that, when applying caps, a proper tray be used to protect the bottom of the tubes from excessive force that is typically used.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use lower volumes in my 96-well Piko PCR Plate than I could in a conventional 96-well microplate?

Yes. Typically, you should be able to use less than half the volume that you currently use on your conventional cycler and achieve equivalent or better results.

How do I know whether I need to purchase the frames for my Piko PCR Plates?

The frames for the Piko PCR Plates allow for easier handling and storage, particularly if you wish to use these plates in conjunction with laboratory equipment designed for microplates (such as centrifuges, plate readers or liquid handlers). Also, the frames allow the ability to stack the plates in a stable and compact fashion for archiving or storage.

Which plastic consumables should I use on my real-time PCR (qPCR)/thermal cycler (PCR) instrument?

Please select your real-time PCR (qPCR) or thermal cycler (PCR) instrument model from the drop-down menu in the qPCR and PCR Plastics Selection Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html) to see the list of all validated plastic consumables (plates, tube strips, single tubes, caps, seals, adhesive films, and accessories) we offer that are compatible with your instrument.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.