MassRuler Express HR Forward DNA Ladder, ready-to-use - FAQs

查看更多产品信息 MassRuler Express HR Forward DNA Ladder, ready-to-use - FAQs (SM1243)

4 个常见问题解答

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.

Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.