RiboRuler High Range RNA Ladder, ready-to-use - FAQs

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

Can I use ready-to-use RiboRuler RNA Ladders in native gel electrophoresis?

Ready-to-use RiboRuler RNA Ladders are not suitable for native gel electrophoresis since they are in a loading buffer that contains 47.5% formamide and 0.0125% SDS.

What ladder do you recommend for running RNA on Invitrogen E-Gel EX Agarose Gels?

Depending on the RNA fragment size, we recommend the following ladders:

• RiboRuler Low Range RNA ladder, (100 bp-1,000 bp) (Cat. No. SM1833)


• RiboRuler High Range RNA ladder, (200 bp-6,000 bp) (Cat. No. SM1823)