Piko PCR Plate, 96-well, white, 50 plates - FAQs

查看更多产品信息 Piko PCR Plate, 96-well, white - FAQs (SPL096150, SPL0961)

7 个常见问题解答

Are ultra thin wall (UTW) vessels compatible with real-time experiments?

Yes. All of the UTW plastics we provide are well suited for qPCR reactions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Will ultra thin wall (UTW) vessels automatically speed up reactions on my conventional thermal cycler?

To realize the time savings of the UTW plastics on conventional thermal cyclers, optimization of the protocol is required. This typically involves reducing hold times and changing the actual hold temperatures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Are ultra thin wall (UTW) vessels prone to break more often because of their thinner walls?

When used as instructed, these vessels are remarkably robust and can handle typical laboratory conditions, without fracturing or resulting in loss of volume. It is recommended that, when applying caps, a proper tray be used to protect the bottom of the tubes from excessive force that is typically used.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use lower volumes in my 96-well Piko PCR Plate than I could in a conventional 96-well microplate?

Yes. Typically, you should be able to use less than half the volume that you currently use on your conventional cycler and achieve equivalent or better results.

How do I know whether I need to purchase the frames for my Piko PCR Plates?

The frames for the Piko PCR Plates allow for easier handling and storage, particularly if you wish to use these plates in conjunction with laboratory equipment designed for microplates (such as centrifuges, plate readers or liquid handlers). Also, the frames allow the ability to stack the plates in a stable and compact fashion for archiving or storage.

Why does the 96-well Piko PCR Plate have a 6x16 format instead of the typical 8x12 format of microplates?

By using a 6x16 format, the standard multi-channel pipettors and liquid handling instruments can be used without modification.

What is a Piko PCR Plate?

A Piko PCR Plate is a plate for PCR that is equivalent to one-fourth of a standard microplate and is equal to a microscope slide in footprint. These plates conserve all of the standard well-spacing and well-geometry of conventional microplates, e.g., a 96-well Piko PCR Plate has the same 4.5 mm pitch as most conventional 384-well microplates. Up to four Piko PCR Plates can be reversibly snapped into a frame to give the equivalent footprint of a conventional 96-well or 384-well microplate. Piko PCR Plates provide many advantages over traditional microplates: they are available at a lower cost, they accept lower reaction volumes, and require less storage space. In addition, many of the same types of advantages were incorporated in the instrumentation designed to use these plates: the thermal cycler has a smaller footprint, is less expensive, has higher thermal performance specifications, and consumes less power.