Oxoid™ Laked Horse Blood Supplement

Laked Horse Blood Supplement is a nutrient-rich enrichment supplement used to enhance the cultivation and isolation of fastidious Gram-positive and Gram-negative microorganisms. The laking process releases essential growth factors, including hemin (X factor), NAD, and other intracellular nutrients, making it ideal for enriching basal media used in clinical, diagnostic, and research microbiology laboratories.

The supplement supports the growth of clinically important pathogens such as Corynebacterium spp. (including C. diphtheriae, C. ulcerans, and C. pseudotuberculosis), Haemophilus influenzae, Campylobacter spp., and Staphylococcus aureus.

By providing the nutrients required for demanding organisms, it improves the recovery and isolation of fastidious bacteria from clinical specimens and supports applications including pathogen identification, epidemiological surveillance, antimicrobial susceptibility testing, and microbiological research.

Laked horse blood is hemolyzed blood used in culture media, created by freezing and thawing red blood cells to break them open. Laked Horse Blood is a very dark colored liquid. The lytic agent used for lysed horse blood is white saponin that does not affect the growth of bacteria at the level required for lysis, the blood lysis by the addition of Saponin around 6.5% of final concentration.

Typical Formula is Horse Blood (100 mL) and Saponin

Laked Horse Blood is intended to be used in conjunction with:

• Columbia Blood Agar Base (Cat No. CM0331B/Y/W/L/K/R/T)
• Blood Agar Base No. 2 (Cat No. CM0271B/K/R/T)
• Campylobacter Agar Base (Cat No. CM0689B)
• GC Agar Base (Cat No. CM0367B)
• Hoyle Medium Base (Cat No. CM0083B)

Because this product is strictly used in research and clinical laboratory settings, it is sourced from controlled veterinary herds and shipped frozen.

Storage

• Store product in its original packaging between 2°C and 8°C.
• Keep container tightly closed.
• The product may be used until the expiry date stated on the label.
• Protect from moisture.
• Store away from light.
• Allow product to equilibrate to room temperature before use.