Will ThiolTracker Violet bind to proteins as well as glutathione?
Yes, ThiolTracker Violet will bind to any accessible sulfhydryl group (-SH; cysteine) whether it is on reduced glutathione or on the surface of a protein. It will not bind to sulfur bound via a disulfide bond (cystine) or ‘buried’ cysteines.
Detection of intracellular glutathione using ThiolTracker violet stain and fluorescence microscopy.
Authors:Mandavilli BS, Janes MS,
Journal:Curr Protoc Cytom
PubMed ID:20578109
Glutathione plays an important role in protecting mammalian cells from oxidative stress and cell death. Because reduced glutathione (GSH) represents the large majority of intracellular free thiols, cell-permeant, thiol-reactive fluorescent probes represent potentially useful indicators of intracellular GSH. The ThiolTracker Violet stain (a registered trademark of Invitrogen) is a bright ... More
Using acetaminophen's toxicity mechanism to enhance cisplatin efficacy in hepatocarcinoma and hepatoblastoma cell lines.
Authors:Neuwelt AJ, Wu YJ, Knap N, Losin M, Neuwelt EA, Pagel MA, Warmann S, Fuchs J, Czauderna P, Wozniak M,
Journal:Neoplasia
PubMed ID:19794959
BACKGROUND/AIMS: Acetaminophen overdose causes hepatotoxicity mediated by toxic metabolites generated through the cytochrome P450 enzyme. The objective of this study was to investigate whether acetaminophen (AAP) can enhance cisplatin (CDDP) cytotoxicity against human hepatocarcinoma and hepatoblastoma cells in vitro and whether this effect can be prevented by N-acetylcysteine (NAC). METHODS: ... More