Exploring RNA transcription and turnover in vivo by using click chemistry.
AuthorsJao CY, Salic A,
JournalProc Natl Acad Sci U S A
PubMed ID18840688
We describe a chemical method to detect RNA synthesis in cells, based on the biosynthetic incorporation of the uridine analog 5-ethynyluridine (EU) into newly transcribed RNA, on average once every 35 uridine residues in total RNA. EU-labeled cellular RNA is detected quickly and with high sensitivity by using a copper ... More
Approach to profile proteins that recognize post-translationally modified histone
AuthorsLi X, Kapoor TM,
JournalJ Am Chem Soc
PubMed ID20141135
Post-translational modifications (PTMs) of histones, proteins onto which DNA is packaged, are involved in many biological processes, including transcription, recombination, and chromosome segregation. As these PTMs can be dynamic, combinatorial, and mediators of weak interactions, the comprehensive profiling of all proteins that recognize histone PTMs is a daunting task. Here ... More
Imaging the lipidome: omega-alkynyl fatty acids for detection and cellular visualization of lipid-modified proteins.
AuthorsHannoush RN, Arenas-Ramirez N,
JournalACS Chem Biol
PubMed ID19505150
Fatty acylation or lipid modification of proteins controls their cellular activation and diverse roles in physiology. It mediates protein-protein and protein-membrane interactions and plays an important role in regulating cellular signaling pathways. Currently, there is need for visualizing lipid modifications of proteins in cells. Herein we report novel chemical probes ... More