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引用和文献 (7)
Invitrogen™
羧基四甲基罗丹明 (TAMRA) 炔烃（5-羧基四甲基罗丹明，炔丙基苯酰胺），5-异构体
红色荧光四甲基罗丹明 (TAMRA) 炔烃可通过铜催化点击反应与叠氮化物发生反应。点击化学描述了一类使用两步程序通过生物正交或生物学独特部分来标记和检测目标分子的化学反应。两步反应程序涉及叠氮化物和炔烃形成三唑的铜催化反应。点击反应有多个特性：检测部分之间的反应高效；无需极端温度或溶剂；反应产物稳定；反应组分具有生物惰性；可能最重要的是，无副反应发生 – 标记和检测标签彼此之间选择性和特异性地发生反应。与利用靶向非独特胺和巯基 –了解更多信息
| 货号 | 数量 |
|---|---|
| T10183 | 0.5 mg |
货号 T10183
价格（CNY)
6,619.00
0.5 mg
数量:
0.5 mg
价格（CNY)
6,619.00
0.5 mg
红色荧光四甲基罗丹明 (TAMRA) 炔烃可通过铜催化点击反应与叠氮化物发生反应。点击化学描述了一类使用两步程序通过生物正交或生物学独特部分来标记和检测目标分子的化学反应。两步反应程序涉及叠氮化物和炔烃形成三唑的铜催化反应。点击反应有多个特性：检测部分之间的反应高效；无需极端温度或溶剂；反应产物稳定；反应组分具有生物惰性；可能最重要的是，无副反应发生 – 标记和检测标签彼此之间选择性和特异性地发生反应。与利用靶向非独特胺和巯基 – 官能团的琥珀酰亚胺酯或马来酰亚胺的传统化学反应不同，– 点击化学标记分子可应用于复杂生物样品，并且因背景极低而能够以空前高的灵敏度进行检测。
仅供科研使用。不可用于诊断程序。
规格
化学反应性叠氮化物
检测方法荧光
产品规格实心
标签或染料TAMRA™ 异构体，TMR（四甲基罗丹明）
分子量467.53 Da
产品类型四甲基罗丹明炔烃
数量0.5 mg
反应基团炔烃
反应一部分炔烃
运输条件室温
溶解度DMSO (二甲亚砜)
颜色橙色
标签类型经典染料
产品线Molecular Probes
Unit Size0.5 mg
内容与储存
储存于 ≤-20°C 干燥环境下，避光。
常见问题解答 (FAQ)
I am using Click-iT AHA (L-azidohomoalanine) kit to label nascent proteins in live cells, then detecting with TAMRA alkyne after fixation and permeabilization and a click reaction. But I'm seeing nucleolar labeling in the cells. It this expected?
引用和文献 (7)
引用和文献
Abstract
Protein synthesis in distal axons is not required for growth cone responses to guidance cues.
Journal:J Neurosci
PubMed ID:19158291
'Recent evidence suggests that growth cone responses to guidance cues require local protein synthesis. Using chick neurons, we investigated whether protein synthesis is required for growth cones of several types to respond to guidance cues. First, we found that global inhibition of protein synthesis stops axonal elongation after 2 h.
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Journal:J Am Chem Soc
PubMed ID:18683930
'We report an advanced chemoenzymatic labeling strategy for direct fluorescence detection of O-GlcNAc proteins in gels that facilitates proteomic studies and greatly extend the reach of existing technologies. These new tools also enable the expression and dynamics of O-GlcNAc modifications to be monitored by imaging in cells and tissues.
The cytoplasmic tail dileucine motif LL572 determines the glycosylation pattern of membrane-type 1 matrix metalloproteinase.
Journal:J Biol Chem
PubMed ID:18955496
'Membrane-type 1 matrix metalloproteinase (MT1-MMP; MMP-14) drives fundamental physiological and pathological processes, due to its ability to process a broad spectrum of substrates. Because subtle changes in its activity can produce profound physiological effects, MT1-MMP is tightly regulated. Currently, many aspects of this regulation remain to be elucidated. It has
A novel approach to tag and identify geranylgeranylated proteins.
Journal:Electrophoresis
PubMed ID:19784953
A recently developed proteomic strategy, the
New insights into metabolic signaling and cell survival: the role of beta-O-linkage of N-acetylglucosamine.
Journal:J Pharmacol Exp Ther
PubMed ID:18768779
The involvement of glucose in fundamental metabolic pathways represents a core element of biology. Late in the 20th century, a unique glucose-derived signal was discovered, which appeared to be involved in a variety of cellular processes, including mitosis, transcription, insulin signaling, stress responses, and potentially, Alzheimer's disease, and diabetes. By