Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL.
AuthorsNguyen HT, Geens M, Mertzanidou A, Jacobs K, Heirman C, Breckpot K, Spits C,
JournalMol Hum Reprod
PubMed ID24217388
Gain of 20q11.21 is a chromosomal abnormality that is recurrently found in human pluripotent stem cells and cancers, strongly suggesting that this mutation confers a proliferative or survival advantage to these cells. In this work we studied three human embryonic stem cell (hESC) lines that acquired a gain of 20q11.21 ... More
STAT3 and MCL-1 associate to cause a mesenchymal epithelial transition.
AuthorsRenjini AP, Titus S, Narayan P, Murali M, Kumar Jha R, Laloraya M,
Journal
PubMed ID24481815
'Embryo implantation is effected by a myriad of signaling cascades acting on the embryo-endometrium axis. Here we show, by using MALDI TOF analysis, far-western analysis and colocalization and co-transfection studies, that STAT3 and MCL-1 are interacting partners during embryo implantation. We show in vitro that the interaction between the two ... More
Kv3.3 channels harbouring a mutation of spinocerebellar ataxia type 13 alter excitability and induce cell death in cultured cerebellar Purkinje cells.
AuthorsIrie T, Matsuzaki Y, Sekino Y, Hirai H,
Journal
PubMed ID24218544
'Abstract The cerebellum plays crucial roles in controlling sensorimotor functions. The neural output from the cerebellar cortex is transmitted solely by Purkinje cells (PCs), whose impairment causes cerebellar ataxia. Spinocerebellar ataxia type 13 (SCA13) is an autosomal dominant disease, and SCA13 patients exhibit cerebellar atrophy and cerebellar symptoms. Recent ... More
Mutations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination.
AuthorsOhara M, Funyu Y, Ebara S, Sakamoto Y, Seki R, Iijima K, Ohishi A, Kobayashi J, Komatsu K, Tachibana A, Tauchi H,
Journal
PubMed ID24614819
Ionizing radiation induces DNA double-strand breaks (DSBs). Mammalian cells repair DSBs through multiple pathways, and the repair pathway that is utilized may affect cellular radiation sensitivity. In this study, we examined effects on cellular radiosensitivity resulting from functional alterations in homologous recombination (HR). HR was inhibited by overexpression of the ... More
EZH2 overexpression in natural killer/T-cell lymphoma confers growth advantage independently of histone methyltransferase activity.
AuthorsYan J, Ng SB, Tay JL, Lin B, Koh TL, Tan J, Selvarajan V, Liu SC, Bi C, Wang S, Choo SN, Shimizu N, Huang G, Yu Q, Chng WJ,
JournalBlood
PubMed ID23529930
The role of enhancer of zeste homolog 2 (EZH2) in cancer is complex and may vary depending on the cellular context. We found that EZH2 is aberrantly overexpressed in the majority of natural killer/T-cell lymphoma (NKTL), an aggressive lymphoid malignancy with very poor prognosis. We show that EZH2 upregulation is ... More