Evaluation of biotin-dye conjugates for use in an HPLC assay to assess relative binding of biotin derivatives with avidin and streptavidin.
Authors:Wilbur DS, Pathare PM, Hamlin DK, Frownfelter MB, Kegley BB, Leung WY, Gee KR
Journal:Bioconjug Chem
PubMed ID:10898581
In this investigation, studies were conducted to determine if size exclusion HPLC could be used to assess relative association rates (on-rates) and dissociation rates (off-rates) of biotin derivatives from avidin (Av) and streptavidin (SAv). For easy detection and quantification of biotin derivatives, molecules that can be detected by UV absorbance ... More