FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)
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FM&trade; 4-64 Dye (<i>N</i>-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)
Citations & References (319)
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FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)

The styryl dye FM 4-64 has been reported to selectively stain yeast vacuolar membranes with red fluorescence (excitation/emission maxima ∼515/640Read more
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Catalog NumberQuantity
T1332010 x 100μg
T31661mg
Catalog number T13320
Price (CNY)
6,296.00
Online Exclusive
Ends: 31-Dec-2025
8,533.00
Save 2,237.00 (26%)
10 x 100 µg
Add to cart
Quantity:
10 x 100μg
Price (CNY)
6,296.00
Online Exclusive
Ends: 31-Dec-2025
8,533.00
Save 2,237.00 (26%)
10 x 100 µg
Add to cart
The styryl dye FM 4-64 has been reported to selectively stain yeast vacuolar membranes with red fluorescence (excitation/emission maxima ∼515/640 nm). This lipophilic dye is an important tool for visualizing vacuolar organelle morphology and dynamics, for studying the endocytic pathway and for screening and characterizing yeast endocytosis mutants. FM 4-64 is also available in special packaging with 10 vials of 100 μg each (T-13320).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineFM
Quantity10 x 100μg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationPlasma Membrane
Unit Size10 x 100 µg
Contents & Storage
Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (319)

Citations & References
Abstract
Apg9p/Cvt7p is an integral membrane protein required for transport vesicle formation in the Cvt and autophagy pathways.
Authors:Noda T,Kim J,Huang WP,Baba M,Tokunaga C,Ohsumi Y,Klionsky DJ
Journal:The Journal of cell biology
PubMed ID:10662773
In nutrient-rich, vegetative conditions, the yeast Saccharomyces cerevisiae transports a resident protease, aminopeptidase I (API), to the vacuole by the cytoplasm to vacuole targeting (Cvt) pathway, thus contributing to the degradative capacity of this organelle. When cells subsequently encounter starvation conditions, the machinery that recruited precursor API (prAPI) also sequesters ... More
Glutamate induces the rapid formation of spine head protrusions in hippocampal slice cultures.
Authors:Richards DA,Mateos JM,Hugel S,de Paola V,Caroni P,Gähwiler BH,McKinney RA
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:15831587
Synaptic plasticity at neuronal connections has been well characterized functionally by using electrophysiological approaches, but the structural basis for this phenomenon remains controversial. We have studied the dynamic interactions between presynaptic and postsynaptic structures labeled with FM 4-64 and a membrane-targeted GFP, respectively, in hippocampal slices. Under conditions of reduced ... More
Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis.
Authors:Gary JD,Wurmser AE,Bonangelino CJ,Weisman LS,Emr SD
Journal:The Journal of cell biology
PubMed ID:9763421
The Saccharomyces cerevisiae FAB1 gene encodes a 257-kD protein that contains a cysteine-rich RING-FYVE domain at its NH2-terminus and a kinase domain at its COOH terminus. Based on its sequence, Fab1p was initially proposed to function as a phosphatidylinositol 4-phosphate (PtdIns(4)P) 5-kinase (). Additional sequence analysis of the Fab1p kinase ... More
Slow spontaneous secretion from single large dense-core vesicles monitored in neuroendocrine cells.
Authors:Stenovec M, Kreft M, Poberaj I, Betz WJ, Zorec R
Journal:FASEB J
PubMed ID:15180959
Hormones are released from cells by passing through an exocytotic pore that forms after vesicle and plasma membrane fusion. In stimulated exocytosis vesicle content is discharged swiftly. Although rapid vesicle discharge has also been proposed to mediate basal secretion, this has not been studied directly. We investigated basal hormone release ... More
Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator.
Authors:Fu L, Sztul E
Journal:J Cell Biol
PubMed ID:12538638
'Newly synthesized proteins that do not fold correctly in the ER are targeted for ER-associated protein degradation (ERAD) through distinct sorting mechanisms; soluble ERAD substrates require ER-Golgi transport and retrieval for degradation, whereas transmembrane ERAD substrates are retained in the ER. Retained transmembrane proteins are often sequestered into specialized ER ... More
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