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Citations & References (42)
Invitrogen™
Transferrin From Human Serum, Alexa Fluor™ 594 Conjugate
Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cellsRead more
| Catalog Number | Quantity |
|---|---|
| T13343 | 5 mg |
Catalog number T13343
Price (CNY)
9,191.00
5 mg
Quantity:
5 mg
Price (CNY)
9,191.00
5 mg
Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.
These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.
Transferrin Specifications:
• Label (Ex/Em): Alexa Fluor™ 594 (590/617)
• Amount: 15 mg solid (contains 5 mg of transferrin conjugate)
Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.
Transferrin Specifications:
• Label (Ex/Em): Alexa Fluor™ 594 (590/617)
• Amount: 15 mg solid (contains 5 mg of transferrin conjugate)
Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeAlexa Fluor Dyes
Excitation/Emission590/617
FormSolid
Protein FamilyTransferrin
Quantity5 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor
Product TypeTransferrin
pH7.2
Unit Size5 mg
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (42)
Citations & References
Abstract
Inhibition of caveolar uptake, SV40 infection, and beta1-integrin signaling by a nonnatural glycosphingolipid stereoisomer.
Journal:J Cell Biol
PubMed ID:17371832
'Caveolar endocytosis is an important mechanism for the uptake of certain pathogens and toxins and also plays a role in the internalization of some plasma membrane (PM) lipids and proteins. However, the regulation of caveolar endocytosis is not well understood. We previously demonstrated that caveolar endocytosis and beta1-integrin signaling are
The long and the short cycle. Alternative intracellular routes for trafficking of G-protein-coupled receptors.
Journal:J Biol Chem
PubMed ID:11150299
'The C terminus of the human V2 vasopressin receptor contains multiple phosphorylation sites including a cluster of amino acids that when phosphorylated prevents the return of the internalized receptor to the cell surface. To identify the step where the recycling process was interrupted, the trafficking of the V2 receptor was
Transfer of M2 muscarinic acetylcholine receptors to clathrin-derived early endosomes following clathrin-independent endocytosis.
Journal:J Biol Chem
PubMed ID:12093817
'Upon agonist stimulation, many G protein-coupled receptors such as beta(2)-adrenergic receptors are internalized via beta-arrestin- and clathrin-dependent mechanisms, whereas others, like M(2) muscarinic acetylcholine receptors (mAChRs), are internalized by clathrin- and arrestin-independent mechanisms. To gain further insight into the mechanisms that regulate M(2) mAChR endocytosis, we investigated the post-endocytic trafficking
Identification and characterization of small molecules that inhibit intracellular toxin transport.
Journal:Infect Immun
PubMed ID:17576758
'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins
A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16798879
'Clathrin-coated pits assemble on a membrane and pinch off as coated vesicles. The released vesicles then rapidly lose their clathrin coats in a process mediated by the ATPase Hsc70, recruited by auxilin, a J-domain-containing cofactor. How is the uncoating process regulated? We find that during coat assembly small and variable