Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
AuthorsDriessen AJ, van den Hooven HW, Kuiper W, van de Kamp M, Sahl HG, Konings RN, Konings WN
JournalBiochemistry
PubMed ID7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent ... More
Investigation of the lipid domains and apolipoprotein orientation in reconstituted high density lipoproteins by fluorescence and IR methods.
AuthorsWald JH, Coormaghtigh E, De Meutter J, Ruysschaert JM, Jonas A
JournalJ Biol Chem
PubMed ID2123199
'The reconstituted high density lipoproteins (rHDL) that were described in the preceding paper (Hefele Wald, J., Krul, E. S., and Jonas, A. (1990) J. Biol. Chem. 265, 20037-20043) are used in this study to analyze the organization, conformation, and dynamics of the lipid phase, as well as the relative orientation ... More
Contribution of hydrophobicity of nonionic detergents to membrane lipid fluidity and disopyramide uptake by rat intestinal brush-border membrane vesicles.
AuthorsKoga K, Murakami M, Kawashima S
JournalBiol Pharm Bull
PubMed ID9212989
'The contribution of hydrophobicity of different types of detergents to disopyramide uptake by rat small intestinal brush-border membrane vesicles was studied in relation to their membrane lipid fluidity and the physicochemical parameters of the detergents, i.e., hydrophile-lipophile balance (HLB). Span-, Tween-type detergents or glycerol esters at non-solubilizing concentrations (0.01-0.05% (w/v)) ... More
ADH modulates plasma membrane lipid order of living MDCK cells via a cAMP-dependent process.
AuthorsGiocondi MC, Friedlander G, Le Grimellec C
JournalAm J Physiol
PubMed ID2165361
'Using 1-[4-(trimethylamino)phenyl]-6-phenyl-hexa-1,3,5-triene, a fluorescent probe that specifically labels the external leaflet of the plasma membrane of living cells, we examined the effects of antidiuretic hormone (ADH) and various agents known to raise intracellular adenosine 3'',5''-cyclic monophosphate (cAMP) on the physical state of the plasma membrane of Madin-Darby canine kidney (MDCK) ... More
Vesicle-mediated phosphatidylcholine reapposition to the plasma membrane following hormone-induced phospholipase D activation.
AuthorsColetti D, Silvestroni L, Naro F, Molinaro M, Adamo S, Palleschi S
JournalExp Cell Res
PubMed ID10739656
'Phospholipase D (PLD) activation involved in signal transduction may lead to the hydrolysis of conspicuous amounts of phosphatidylcholine (PC). This study shows that PLD activation significantly alters the plasma membrane (PM) environment and the membrane exchange dynamics. PC-PLD activation in vasopressin (AVP)-stimulated L6 myogenic cells was accompanied by increased exocytosis ... More
Fluorescence depolarization of cis- and trans-parinaric acids in artificial and red cell membranes resolved by a double hindered rotational model.
AuthorsCalafut TM, Dix JA, Verkman AS
JournalBiochemistry
PubMed ID2765524
'Although steady-state anisotropy measurements of phase-sensitive probes provide a qualitative description of the phase behavior of biomembranes, there is little information about the physical state of lipid domains. We have developed a ground-state double hindered rotator model (DHR) for fluorescence anisotropy decay, in which probes possess separate rotational correlation times ... More
Synergistic activation of the human red cell calcium ATPase by magnesium and vanadate.
AuthorsRomero PJ
JournalBiochim Biophys Acta
PubMed ID8499454
'The Ca(2+)-ATPase activity of human red cells was studied on calmodulin-free membrane fragments after previous incubation with Mg2+ and vanadate. In the presence of EGTA (5 mM), the activity was slightly affected by either ion alone. However, when added together, both Ca2+ affinity and Vmax were increased up to levels ... More
Fluidity changes and chemical composition of lipoproteins in type IIa hyperlipoproteinemia.
AuthorsDachet C, Motta C, Neufcour D, Jacotot B
JournalBiochim Biophys Acta
PubMed ID2397246
'The chemical composition and the physical properties of lipoproteins (VLDL, LDL and HDL) were studied in two groups of patients: 14 healthy normolipidemic subjects and 15 type IIa familial hypercholesterolemic patients. The steady-state fluorescence anisotropy rs was estimated in lipoproteins by the fluorescence depolarization of two fluorescent probes: the DPH ... More
Thioridazine interacts with the membrane of mitochondria acquiring antioxidant activity toward apoptosis--potentially implicated mechanisms.
AuthorsRodrigues T, Santos AC, Pigoso AA, Mingatto FE, Uyemura SA, Curti C
JournalBr J Pharmacol
PubMed ID11976278
'We evaluated the effects of the phenothiazine derivative thioridazine on mechanisms of mitochondria potentially implicated in apoptosis, such as those involving reactive oxygen species (ROS) and cytochrome c release, as well as the involvement of drug interaction with mitochondrial membrane in these effects. Within the 0 - 100 microM range ... More
Effects of culture and incubation conditions on membrane fluidity in monolayers of cultured cells measured as fluorescence anisotropy using trimethylammoniumdiphenylhexatriene (TMA-DPH).
AuthorsToplak H, Batchiulis V, Hermetter A, Hunziker T, Honegger UE, Wiesmann UN
JournalBiochim Biophys Acta
PubMed ID2207120
'Membrane fluidity of coverslip attached living cells was measured as fluorescence anisotropy using 5 microM trimethylammoniumdiphenylhexatriene (TMA-DPH) as fluorescent probe. Fluorescence anisotropy is inversely related to membrane fluidity. Cells were grown on glass coverslips that were inserted and directly incubated in quarz cuvettes. The coverslips were fixed with special holders ... More
Location of diphenylhexatriene (DPH) and its derivatives within membranes: comparison of different fluorescence quenching analyses of membrane depth.
AuthorsKaiser RD, London E
JournalBiochemistry
PubMed ID9609714
'The average membrane location of a series of diphenylhexatriene (DPH)-derived membrane probes was analyzed by measuring the quenching of DPH fluorescence with a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. All DPH derivatives were located deeply within the bilayer. Some derivatives were anchored ... More
Modification of ceftibuten transport by the addition of non-ionic surfactants.
AuthorsKoga K, Ohyashiki T, Murakami M, Kawashima S
JournalEur J Pharm Biopharm
PubMed ID10613923
'The effects of non-ionic surfactants on the carrier-mediated transport of ceftibuten by rat intestinal brush-border membrane vesicles (BBMVs) were investigated. Ceftibuten uptake by BBMVs was measured by a rapid filtration technique. The concentration of surfactants for the uptake experiments was determined by a decrease in the turbidity of BBMV suspension ... More
Advantages and limitations of 1-palmitoyl-2-[[2-[4- (6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl]carbonyl]-3- sn-phosphatidylcholine as a fluorescent membrane probe.
AuthorsParente RA, Lentz BR
JournalBiochemistry
PubMed ID4084512
'We have investigated the behavior of 1-palmitoyl-2-[[2-[4- (6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl]carbonyl]-3-sn -phosphatidylcholine (DPHpPC) in synthetic, multilamellar phosphatidylcholine vesicles. This fluorescent phospholipid has photophysical properties similar to its parent fluorophore, diphenylhexatriene (DPH). DPHpPC preferentially partitioned into fluid phase lipid (Kf/s = 3.3) and reported a lower phase transition temperature as detected by fluorescence anisotropy ... More
Gangliosides enhance the membrane actions of ethanol and pentobarbital.
'The physical properties of vesicles containing phosphatidylcholine (PC) and gangliosides were evaluated by the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene, trans- parinarate (probes of the lower acyl regions), and 1-(4- trimethylammoniumphenyl )-6-phenyl-1,3,5-hexatriene (a probe of the glycerol backbone and upper acyl regions). Exposure of PC vesicles to ethanol produced a decrease in polarization ... More
TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells.
AuthorsKuhry JG, Fonteneau P, Duportail G, Maechling C, Laustriat G
JournalCell Biophys
PubMed ID6197175
'Fluorescence intensity measurements and fluorescence microscopy data showed that TMA-DPH (trimethylammonium diphenylhexatriene), a cationic derivative of the fluorescence polarization probe DPH, has a considerably different behavior in L929 cultured cells than does its parent molecule. In contrast to DPH, it incorporates very rapidly in the plasma membranes of the treated ... More
Membrane labelling by fluorescent probes: incorporation of TMA-DPH in erythrocyte membranes.
AuthorsMaire C, Bouchy M, Donner M, André JC
JournalBiorheology
PubMed ID1306379
'A study of the labelling of isolated resealed erythrocyte membranes by TMA-DPH has been carried out. A quantitative study shows that saturation appears to take place when increasing the relative quantity of probe bulk concentration to membrane concentration; this is readily interpreted by a simple incorporation model with a limited ... More
Relevance of drug uptake, cellular distribution and cell membrane fluidity to the enhanced sensitivity of Down's syndrome fibroblasts to anticancer antibiotic-mitoxantrone.
AuthorsPrzybylska M, Józwiak Z
JournalBiochim Biophys Acta
PubMed ID12659957
'Sensitivity of human fibroblasts derived from Down''s syndrome (DS) individuals (S-240, T-158, T-74, T-164) and normal donors (S-126, WA-1) to anticancer antibiotic-mitoxantrone (1,4-dihydroxy-5,8-bis((2-((2-hydroxy-ethyl)amino)ethyl)amino)-9,10-anthracenedione dihydrochloride; MIT) and its relationship to the transport rate, cellular distribution and interaction with cell membrane were studied. The survival assay showed that MIT was more toxic ... More
Membrane lipid dynamics in human promyelocytic leukemia cells sensitive and resistant to 12-O-tetradecanoylphorbol-13-acetate induction of differentiation.
AuthorsFisher PB, Schachter D, Abbott RE, Callaham MF, Huberman E
JournalCancer Res
PubMed ID6594192
'A series of fluorescent probes was used to analyze membrane lipid dynamics in promyelocytic leukemic cells sensitive (HL-60) or resistant (R-55) to phorbol diester induction of cell differentiation. When examined with the probe 1,6-diphenyl-1,3,5-hexatriene, which can penetrate the plasma membrane and intercalate in the lipids of both leaflets of the ... More
The influence of cholesteryl ester transfer protein on the composition, size, and structure of spherical, reconstituted high density lipoproteins.
AuthorsRye KA, Hime NJ, Barter PJ
JournalJ Biol Chem
PubMed ID7814372
'The effect of cholesteryl ester transfer protein (CETP) on the size, composition, and structure of spherical, reconstituted HDL (rHDL) which contain apolipoprotein (apo) A-I as their sole apolipoprotein has been studied. Spherical rHDL were incubated with CETP and Intralipid for up to 24 h. During this time CETP promoted transfers ... More
[The effect of biologically active compounds on lysosome fusion with phagosomes and the F-actin content in mouse peritoneal macrophages and on the status of the lysosomal membranes in mouse hepatocytes]
'Effects of biologically active compounds bilirubin (BR, 0.1 and 0.2 mM), chelerythrine (CR, 0.1 and 0.5 mM) and farmorubicin (FR, 0.6 and 6.0 mM) on the phagosome-lysosome fusion (P-LF) were studied using fluorescent dye acridine orange for lysosomal labelling and yeast cells as a target. To investigate mechanisms of these ... More
Activation of human aortic endothelial cells by LDL from Type 1 diabetic patients: an in vitro study.
AuthorsRabini RA, Vignini A, Salvolini E, Staffolani R, Martarelli D, Moretti N, Mazzanti L
JournalAtherosclerosis
PubMed ID12208472
'An altered interaction between circulating LDL and endothelial cells might be at the basis of the increased prevalence of atherosclerosis in diabetes mellitus. The aim of the present work was to investigate the effect of a short incubation period with LDL from Type 1 diabetic patients in good metabolic control ... More
Lipid peroxidation of the brush-border membrane: membrane physical properties and glucose transport.
AuthorsJourd'Heuil D, Vaananen P, Meddings JB
JournalAm J Physiol
PubMed ID8333526
'During inflammatory conditions, peroxidation of biological membranes often takes place. Deleterious physiological consequences, in terms of membrane function, could theoretically be mediated by either direct oxidative attack upon integral membrane proteins or by indirectly altering the lipid environment surrounding these proteins. To address this issue, in vitro peroxidation of guinea ... More
Membrane fluidity changes in P. berghei-infected erythrocytes, investigated with a specific plasma membrane fluorescent probe.
AuthorsDeguercy A, Schrevel J, Duportail G, Laustriat G, Kuhry JG
JournalBiochem Int
PubMed ID3511915
'Trimethylamino-diphenylhexatriene (TMA-DPH), a novel hydrophobic fluorescent probe with relevant photophysical properties for fluorescence anisotropy measurements in phospholipidic membranes, specifically labels the plasma membranes of whole living-cells, unlike earlier commonly used probes such as 1,6-diphenyl-1,3,5-hexatriene (DPH) and anthroyloxy fatty acids, which invade all hydrophobic regions of the cell. Using TMA-DPH, it ... More
Lipid composition determines the effects of arbutin on the stability of membranes.
AuthorsHincha DK, Oliver AE, Crowe JH
JournalBiophys J
PubMed ID10512822
'Arbutin (hydroquinone-beta-D-glucopyranoside) is an abundant solute in the leaves of many freezing- or desiccation-tolerant plants. Its physiological role in plants, however, is not known. Here we show that arbutin protects isolated spinach (Spinacia oleracea L.) thylakoid membranes from freeze-thaw damage. During freezing of liposomes, the presence of only 20 mM ... More
Steady-state fluorescence studies on lipase-vesicle interactions.
AuthorsMosmuller EW, Pap EH, Visser AJ, Engbersen JF
JournalBiochim Biophys Acta
PubMed ID8305458
'The interaction of lipase from Candida cylindracea (CCL) with positively charged polymerizable surfactant vesicles was studied by the use of steady-state fluorescence techniques. The phase transition of vesicles composed of nonpolymerized and polymerized N-allylbis[2-(hexadecanoyloxy)ethyl]methylammonium bromide (ABHEMA Br) was determined in the absence of lipase, by measuring the change in fluorescence ... More
Evaluation of hydralazine and procainamide effects on fibroblast membrane fluidity.
AuthorsWeglarz L, Koceva-Chyla A, Gwozdzinski K, Dzierzewicz Z, Józwiak Z
JournalBiochimie
PubMed ID12763314
'In this study the membrane fluidity of fibroblasts under different pharmacological treatment was investigated. Two drugs, hydralazine and procainamide, were used to treat the immortalized mouse NIH 3T3 and hamster B14 fibroblasts. Membrane lipid dynamics was measured by fluorescence spectroscopy and electron spin resonance techniques. Two kinds of fluorescent probes ... More
Diacylglycerol and the promotion of lamellar-hexagonal and lamellar-isotropic phase transitions in lipids: implications for membrane fusion.
AuthorsBasanez G, Nieva JL, Rivas E, Alonso A, Goni FM
JournalBiophys J
PubMed ID9172753
'Changes in steady-state fluorescence anisotropy of 1 -(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene TMA-DPH) are applied to the detection of lamellar-hexagonal transitions in egg phosphatidylethanolamine. Even low (2 mole%) proportions of diacylglycerol decrease the hexagonal transition temperature considerably, as confirmed by differential scanning calorimetry. Diacylglycerol is also found to promote a lamellar to "isotropic" (Q(224) ... More
Comparison between flow cytometry and fluorometry for the kinetic measurement of membrane fluidity parameters.
AuthorsCollins JM, Grogan WM
JournalCytometry
PubMed ID2917474
'Steady-state fluorescence polarization measurements obtained with a flow cytometer were compared with those obtained with an SLM subnanosecond fluorometer. Measurements were made over time after exposure of HeLa cells to the membrane probe 1,6-diphenyl-1,3,5-hexatriene (DPH), 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), or [12-(9:anthroyloxy) stearate (12-AS). After 1 min, anisotropy values of 0.28 (DPH), 0.28 ... More
Imaging peptidoglycan biosynthesis in Bacillus subtilis with fluorescent antibiotics.
AuthorsTiyanont K, Doan T, Lazarus MB, Fang X, Rudner DZ, Walker S
JournalProc Natl Acad Sci U S A
PubMed ID16832063
'The peptidoglycan (PG) layers surrounding bacterial cells play an important role in determining cell shape. The machinery controlling when and where new PG is made is not understood, but is proposed to involve interactions between bacterial actin homologs such as Mbl, which forms helical cables within cells, and extracellular multiprotein ... More
Effects of melittin on molecular dynamics and Ca-ATPase activity in sarcoplasmic reticulum membranes: time-resolved optical anisotropy.
AuthorsVoss J, Birmachu W, Hussey DM, Thomas DD
JournalBiochemistry
PubMed ID1649630
'We have studied the effect of melittin, a basic membrane-binding peptide, on Ca-ATPase activity and on protein and lipid dynamics in skeletal sarcoplasmic reticulum (SR), using time-resolved phosphorescence and fluorescence spectroscopy. Melittin completely inhibits Ca-ATPase activity, with half-maximal inhibition at 9 +/- 1 mol of melittin bound to the membrane ... More
Partition coefficients of fluorescent probes with phospholipid membranes.
AuthorsHuang ZJ, Haugland RP
JournalBiochem Biophys Res Commun
PubMed ID1958185
'A method for determination of membrane partition coefficients of five fluorescent membrane probes, 1,6-diphenyl-1,3,5-hexatriene (DPH), p-((6-phenyl)-1,3,5-hexatrienyl) benzoic acid (DPH carboxylic acid), 3-(p-(6-phenyl)-1,3,5-hexatrienyl)phenylpropionic acid (DPH propionic acid), 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and N-4-(4-didecylaminostyryl)-N-methylpyridinium iodide (4-di-10-ASP), was developed utilizing the fluorescence enhancement of a constant probe concentration by titration with excess phospholipid liposomes. The ... More
Effects of alcohols on fluorescence anisotropies of diphenylhexatriene and its derivatives in bovine blood platelets: relationships of the depth-dependent change in membrane fluidity by alcohols with their effects on platelet aggregation and adenylate cyclase activity.
AuthorsKitagawa S, Hirata H
JournalBiochim Biophys Acta
PubMed ID1329963
'The effects of three short-chain alkyl alcohols and benzyl alcohol on the membrane fluidity of bovine blood platelets were investigated by studies on the fluorescence anisotropies of diphenylhexatriene (DPH), its cationic trimethylammonium derivative (TMA-DPH) and its anionic propionic acid derivative (DPH-PA). These alcohols decreased the fluorescence anisotropy of DPH, which ... More
Bioenergetics of the staphylococcal multidrug export protein QacA. Identification of distinct binding sites for monovalent and divalent cations.
AuthorsMitchell BA, Paulsen IT, Brown MH, Skurray RA
JournalJ Biol Chem
PubMed ID9920900
'The multidrug efflux pump QacA from Staphylococcus aureus confers resistance to an extensive range of structurally dissimilar compounds. Fluorimetric analyses demonstrated that QacA confers resistance to the divalent cation 4'',6-diamidino-2-phenylindole, utilizing a proton motive force-dependent efflux mechanism previously demonstrated for QacA-mediated resistance to the monovalent cation ethidium. Both the ionophores ... More
Effect of aluminium on lipid peroxidation of human high density lipoproteins.
AuthorsFerretti G, Marchionni C, Bacchetti T, Galeazzi T, Dousset N
JournalFree Radic Res
PubMed ID12797472
'We investigated the effect of aluminium (Al3+) on lipid peroxidation and physico-chemical properties of high density lipoproteins (HDL) isolated from human plasma. Our results demonstrated that Al3+ enhances lipid peroxidation of human HDL as shown by the significant increase in lipid hydroperoxides in Al-treated HDL with respect to control HDL. ... More
Clathrin polymerization is not required for bulk-phase endocytosis in rat fetal fibroblasts.
AuthorsCupers P, Veithen A, Kiss A, Baudhuin P, Courtoy PJ
JournalJ Cell Biol
PubMed ID7962055
'To assess the role of clathrin in the bulk endocytic flow of rat foetal fibroblasts, the rate of internalization of fluid-phase and membrane-lipid tracers were compared, under control conditions and after inhibition of endocytic clathrin-coated pit formation. After intracellular potassium depletion or upon cell transfer into 0.35 M NaCl, the ... More
Aluminum accumulation and membrane fluidity alteration in synaptosomes isolated from rat brain cortex following aluminum ingestion: effect of cholesterol.
'In the present work, we studied the effect of cholesterol/phospholipid (CH/PL) molar ratio on aluminum accumulation and aluminum-induced alteration of membrane fluidity in rat brain cortex synaptosomes. We observed that sub-acute (daily supply of 1.00 g of AlCl(3) during 10 days) and chronic (daily supply of 0.03 g of AlCl(3) ... More
Effect of hydrostatic pressure on water penetration and rotational dynamics in phospholipid-cholesterol bilayers.
AuthorsBernsdorff C, Wolf A, Winter R, Gratton E
JournalBiophys J
PubMed ID9138572
'The effect of high hydrostatic pressure on the lipid bilayer hydration, the mean order parameter, and rotational dynamics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) cholesterol vesicles has been studied by time-resolved fluorescence spectroscopy up to 1500 bar. Whereas the degree of hydration in the lipid headgroup and interfacial region was ... More
Binding of lecithin:cholesterol acyltransferase to reconstituted high density lipoproteins is affected by their lipid but not apolipoprotein composition.
AuthorsBolin DJ, Jonas A
JournalJ Biol Chem
PubMed ID8125962
'The reaction of lecithin:cholesterol acyltransferase (LCAT) with high density lipoproteins (HDL) is of critical importance in reverse cholesterol transport. We studied the relationship between LCAT binding and HDL composition using two assays to determine LCAT binding affinity for discoidal reconstituted HDL (rHDL). We prepared rHDL with egg phosphatidylcholine (egg-PC), cholesterol, ... More
NO synthase and xanthine oxidase activities of rabbit brain synaptosomes: peroxynitrite formation as a causative factor of neurotoxicity.
AuthorsDeliconstantinos G, Villiotou V
JournalNeurochem Res
PubMed ID8833224
'In the present study we demonstrated that synaptosomes isolated from rabbit brain cortex contain NO synthase and xanthine oxidase that can be activated by ultraviolet B radiation and Ca2+ accumulation to produce nitric oxide and superoxide which react together to form peroxynitrite. Irradiation of synaptosomes with ultraviolet B (up to ... More
Activation of human lymphocytes by concanavalin A or purified protein derivative results in no alteration of fluorescence polarization of lipid probes although the electrophoretic mobility of the cells is changed.
AuthorsParola AH, Kaplan JH, Lockwood SH, Uzgiris EE
JournalBiochim Biophys Acta
PubMed ID7317420
'Upon stimulation with either concanavalin A or the tuberculin antigen, purified protein derivative, human peripheral blood lymphocytes, purified on Ficoll-Hypaque, did not exhibit a concomitant lipid fluidity alteration as measured by fluorescence polarization (P) of the lipid probe, 1,6-diphenyl-1,3-5-hexatriene (DPH). This result was independent of the incubation period, ranging from ... More
Interaction between microcystins of different hydrophobicities and lipid monolayers.
AuthorsVesterkvist PS, Meriluoto JA
JournalToxicon
PubMed ID12565758
'Microcystins (MC) are a group of amphiphatic peptide hepatotoxins and protein phosphatase inhibitors produced by certain cyanobacteria (blue-green algae). Microcystins are believed to require an active transport mechanism to penetrate the plasma membranes of animal cells. In this study the surface barostat technique showed that two more hydrophobic microcystins MC-LF, ... More
Altered platelet membrane dynamic properties in type 1 diabetes.
AuthorsMazzanti L, Rabini RA, Fumelli P, Martarelli D, Staffolani R, Salvolini E, Curatola G
JournalDiabetes
PubMed ID9392498
'A modified platelet response to aggregating stimuli is supposed to play a role in the pathogenesis of diabetic macroangiopathy. We studied the fluidity and microheterogeneity of the external surface of the platelet membrane and the activities of the plasma membrane Na+-K+-ATPase and Ca2+-ATPase in 21 men with type 1 diabetes ... More
Liquid-ordered microdomains in lipid rafts and plasma membrane of U-87 MG cells: a time-resolved fluorescence study.
AuthorsSinha M, Mishra S, Joshi PG
JournalEur Biophys J
PubMed ID12851796
'Lipid rafts, the functional microdomains in the cell membrane, are believed to exist as liquid-ordered (Lo) phase domains along with the liquid-disordered (Ld) phase of the bulk of the cell membranes. We have examined the lipid order in model and natural membranes by time-resolved fluorescence of trimethylammonium-1,6-diphenylhexatriene incorporated into the ... More
A quantitative model for the dependence of solute permeability on peptide and cholesterol content in biomembranes.
AuthorsXiang TX, Chen J, Anderson BD
JournalJ Membr Biol
PubMed ID11003688
'The influence of varying concentrations of a transmembrane peptide, gramicidin A (gA), and cholesterol (Chol) on the passive permeation of p-methylhippuric acid (MHA) and alpha-carbamoyl-p-methylhippuric acid (CMHA) across egg-lecithin membranes (EPC) has been investigated in vesicle efflux experiments. Incorporation of 0.25 volume fraction of gA in its nonchannel conformation increased ... More
Structure and polarity of mouse brain synaptic plasma membrane: effects of ethanol in vitro and in vivo.
AuthorsColles S, Wood WG, Myers-Payne SC, Igbavboa U, Avdulov NA, Joseph J, Schroeder F
JournalBiochemistry
PubMed ID7727452
'Structural and dielectric alteration by ethanol in vitro and chronic ethanol consumption were examined in synaptic plasma membranes (SPM) using diphenylhexatriene and charged diphenylhexatriene derivatives. These fluorophores, in combination with multifrequency phase and modulation fluorometry, allowed the examination of the surface and interior core of SPM. Limiting anisotropy and rotational ... More
Hierarchy of protein assembly at the vertex ring domain for yeast vacuole docking and fusion.
AuthorsWang L, Merz AJ, Collins KM, Wickner W
JournalJ Cell Biol
PubMed ID12566429
'Vacuole tethering, docking, and fusion proteins assemble into a "vertex ring" around the apposed membranes of tethered vacuoles before catalyzing fusion. Inhibitors of the fusion reaction selectively interrupt protein assembly into the vertex ring, establishing a causal assembly hierarchy: (a) The Rab GTPase Ypt7p mediates vacuole tethering and forms the ... More
Change in membrane organization induced by heat shock.
AuthorsRevathi CJ, Chattopadhyay A, Srinivas UK
JournalBiochem Mol Biol Int
PubMed ID8069244
'In vivo change in membrane organization in adult rat liver cells induced by heat shock was studied by incorporating the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-[4-(trimethylammonio)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) in these cells. DPH was essentially localized in the plasma membrane, as indicated by quenching of its fluorescence (over 90%) by 2,4,6-trinitrobenzenesulfonate (TNBS). ... More
Thyroliberin-induced changes in the fluorescence of a membrane probe in individual bovine anterior pituitary cells.
AuthorsShorte SL, Stafford SJ, Bamford M, Collett VJ, Schofield JG
JournalJ Physiol
PubMed ID8308725
'1. We have investigated the use of TMA-DPH (1-[4-(trimethylammonio) phenyl]-6-phenylhexa-1,3,5-triene) as an indicator of exocytosis in individual bovine anterior pituitary cells using microfluorimetric imaging. 2. TMA-DPH was photolabile in artificial and cell membranes. In cells incubated in TMA-DPH the distribution of fluorescence depended both on the incubation time and the ... More
Modifications induced by insulin-dependent diabetes mellitus on human placental Na+/K+-adenosine triphosphatase.
AuthorsZolese G, Rabini RA, Fumelli P, Staffolani R, Curatola A, Kvasnicka P, Kotyk A, Cester N, Mazzanti L
JournalJ Lab Clin Med
PubMed ID9358075
'The causes of the reduced activity of Na+/K+-adenosine triphosphatase (ATPase) in human diabetes are still the object of controversy. The aim of this work was to investigate the mechanisms of inhibition by means of the study of the Na+/K+-ATPase purified from human placenta. We purified Na+/K+-ATPase from term placentas of ... More
TMA-DPH a fluorescent probe of membrane dynamics in living cells. How to use it in phagocytosis.
'Trimethylammonium-diphenylhexatriene (TMA-DPH), a hydrophobic fluorescent probe, has been shown in earlier studies to possess a variety of particular properties in interaction with intact living cells--specific and rapid incorporation into the plasma membrane and partition equilibrium between the membranes and the buffer. These properties offer promising applications in membrane fluidity studies ... More
Brefeldin A (BFA) inhibits basolateral membrane (BLM) delivery and dimerization of transcobalamin II receptor in human intestinal epithelial Caco-2 cells. BFA effects on BLM cholesterol content.
'Brefeldin A (BFA) treatment of Caco-2 cells (5 microg/ml for 12 h) reduced by 90% the cholesterol, but not the phospholipid (PL), levels of the basolateral membrane (BLM), thus altering its PL/cholesterol molar ratio from 2.6 to 22.0, and decreasing its steady state fluorescent anisotropy (rs) from 0.27 to 0.15. ... More
Changes of membrane fluidity in chemotactic peptide-stimulated polymorphonuclear leukocytes.
AuthorsValentino M, Governa M, Fiorini R, Curatola G
JournalBiochem Biophys Res Commun
PubMed ID3814118
'Although the phenomenon of stimulus-response coupling in polymorphonuclear leukocytes involves a series of membrane events the influence of stimulation on membrane fluidity is to clarify. In our experiments we have used 1-(4-trimethylaminophenyl) 6-phenyl-1,3,5-hexatriene and 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization technique to evaluate membrane fluidity in living polymorphonuclear leukocytes after stimulation with N-formyl-methyonil-leucyl-phenylalanine ... More
The effect of transmembrane potential on the dynamic behavior of cell membranes.
AuthorsLakos Z, Somogyi B, Balázs M, Matkó J, Damjanovich S
JournalBiochim Biophys Acta
PubMed ID2317496
'The relationship between transmembrane potential and lipid dynamics in the cytoplasmic membrane of mouse thymus cells has been investigated. Changes of transmembrane potential was followed by measuring the fluorescence emission of the anionic dye, bis-(1,3-dibutylbarbiturate)trimethine oxonol (diBa-C4-(3)). Assessment of lipid fluidity was carried out applying three fluorescent lipid probes, 1-[4-(trimethylammonium)phenyl]-6-phenyl-1,3,5-hexatriene ... More
The influence of sphingomyelin on the structure and function of reconstituted high density lipoproteins.
AuthorsRye KA, Hime NJ, Barter PJ
JournalJ Biol Chem
PubMed ID8626769
'The effect of sphingomyelin (SPM) on the structure and function of discoidal and spherical reconstituted high density lipoproteins (rHDL) has been studied. Three preparations of discoidal rHDL with 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC)/SPM/unesterified cholesterol (UC)/apolipoprotein (apo)A-I molar ratios of 99.6/0. 0/10.2/1.0, 86.0/13.6/10.8/1.0, and 72.5/26.3/11.4/1.0 were prepared by cholate dialysis. SPM did not ... More
Differential effect of tumor promoters on phorbol-ester-receptor binding and membrane fluorescence anisotropy in C3H 10T 1/2 cells.
AuthorsTran PL, Castagna M, Sala M, Vassent G, Horowitz AD, Schachter D, Weinstein LB
JournalEur J Biochem
PubMed ID6297894
Effects of the cannabinoids on physical properties of brain membranes and phospholipid vesicles: fluorescence studies.
AuthorsHillard CJ, Harris RA, Bloom AS
JournalJ Pharmacol Exp Ther
PubMed ID2983062
'The effects of four cannabinoids on the physical properties of brain synaptic plasma membranes (SPM), lipid extracts of SPM and phospholipid vesicles were evaluated using fluorescence probes. In vitro, the psychoactive cannabinoids, delta 9-tetrahydrocannabinol (delta 9-THC) and 11-hydroxyl-delta 9-tetrahydrocannabinol (11-OH-delta 9-THC) at concentrations of 1 and 3 microM decreased polarization ... More
Membrane fluidity of non-activated and activated human blood platelets.
AuthorsFeijge MA, Heemskerk JW, Hornstra G
JournalBiochim Biophys Acta
PubMed ID2364076
'The steady-state fluorescence anisotropy of membranes labeled with 1,6-diphenyl-1,3,5-hexatriene (DPH) or its 4''-trimethylammonio derivative, TMA-DPH, is generally considered a measure for the lipid order and, hence, inversely related to membrane fluidity. We now report that anisotropy values of DPH- and TMA-DPH-labeled human platelets are considerably influenced by experimental conditions like ... More
3-[p-(6-phenyl)-1,3,5-hexatrienyl]phenylpropionic acid (PA-DPH): characterization as a fluorescent membrane probe and binding to fatty acid binding proteins.
AuthorsTrotter PJ, Storch J
JournalBiochim Biophys Acta
PubMed ID2742883
'The negatively charged fluorophore 3-[p-(6-phenyl)-1,3,5-hexatrienyl]phenylpropionic acid (PA-DPH) was characterized by comparison with its parent compound DPH, and with cationic trimethylammonium-DPH (TMA-DPH). The molar absorption coefficient of PA-DPH (60,000 cm-1.mol-1) as well as its quantum yield (0.7) and fluorescence lifetime (5 ns) in fluid phase membranes are intermediate between DPH and ... More
Comparative evolution of endocytosis levels and of the cell surface area during the L929 cell cycle: a fluorescence study with TMA-DPH.
AuthorsIllinger D, Italiano L, Beck JP, Waltzinger C, Kuhry JG
JournalBiol Cell
PubMed ID8004013
'1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene (TMA-DPH), a membrane fluorescence probe, interacts with living cells by instantaneous partition between the external medium and the plasma membrane, where it becomes fluorescent. The corresponding fluorescence intensity is then proportional to the cell surface. On the other hand, once incorporated into the plasma membrane, TMA-DPH follows this membrane ... More
Increased aggregation and secretion responses of human platelets when loaded with the calcium fluorescent probes quin2 and fura-2.
AuthorsLanza F, Beretz A, Kubina M, Cazenave JP
JournalThromb Haemost
PubMed ID3672425
'Incorporation into human platelets of the calcium fluorescent indicators quin2 or fura-2 at low concentrations used to measure intracellular free calcium leads to the potentiation of the effects of agonists on platelets. This was shown by increased aggregatory and secretory responses of quin2 or fura-2 loaded platelets after stimulation with ... More
Membrane structural dynamics of plasma membranes of living human prostatic carcinoma cells differing in metastatic potential.
AuthorsCalderon RO, Grogan WM, Collins JM
JournalExp Cell Res
PubMed ID1893934
'Rigidity of the outer hemileaflet of the plasma membrane of two prostatic carcinoma cell lines with different metastatic potential, 1-LN and 1-LN-EMS-10, was assessed by steady-state anisotropy, using a battery of fluorescent probes. The "bulk" membrane rigidity sensed by diphenylhexatriene, trimethylammonio-DPH, 1-palmitoyl-2-[DPH-ethylcarbonyl]-phosphatidylcholine, and 10-pyrenedecanoic acid indicated slightly higher rigidity in ... More
Surface properties of native human plasma lipoproteins and lipoprotein models.
AuthorsMassey JB, Pownall HJ
JournalBiophys J
PubMed ID9533698
'Plasma lipoprotein surface properties are important but poorly understood determinants of lipoprotein catabolism. To elucidate the relation between surface properties and surface reactivity, the physical properties of surface monolayers of native lipoproteins and lipoprotein models were investigated by fluorescent probes of surface lipid fluidity, surface lateral diffusion, and interfacial polarity, ... More
Differential effect of aging on cholesterol modulation of carbachol-stimulated low-K(m) GTPase in striatal synaptosomes.
AuthorsDenisova NA, Erat SA, Kelly JF, Roth GS
JournalExp Gerontol
PubMed ID9615923
'Previous research has suggested that age-related decline in physiological functions may be the result of substantial alterations in membrane molecular structure. The purpose of the present experiments was to elucidate the role of cholesterol domains in the age-related decline in receptor-G-protein interactions in striatal synaptosomes. We observed a significant age-related ... More
Lipid domains and lipid/protein interactions in biological membranes.
AuthorsTocanne JF, Cézanne L, Lopez A, Piknova B, Schram V, Tournier JF, Welby M
JournalChem Phys Lipids
PubMed ID8001179
'In the fluid mosaic model of membranes, lipids are organized in the form of a bilayer supporting peripheral and integral proteins. This model considers the lipid bilayer as a two-dimensional fluid in which lipids and proteins are free to diffuse. As a direct consequence, both types of molecules would be ... More
Evidence for normal fibroblast cell membranes from individuals with Huntington's disease. A fluorescence probe study.
AuthorsSumbilla C, Lakowicz JR
JournalJ Neurol Sci
PubMed ID6230415
'We used fluorescence spectroscopy in an attempt to identify differences in the fibroblast cell membranes from individuals with Huntington''s disease (HD), compared to those from normal individuals. Eight pairs of age- and sex-matched fibroblast cultures were examined, each using four different fluorescent probes. To maximize the possibility of finding a ... More
Effects of platelet activating factor on calcium-lipid interactions and lateral phase separations in phospholipid vesicles.
AuthorsBratton DL, Harris RA, Clay KL, Henson PM
JournalBiochim Biophys Acta
PubMed ID3401478
'Recent studies localizing the inflammatory mediator, platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), to the membranes of stimulated neutrophils, raise the possibility that PAF may, in addition to its activities as a mediator, alter the physical properties of membranes. This, and the increasing evidence that calcium-lipid interactions may have central importance in ... More
Steady-state fluorescence polarization data in membranes. Resolution into physical parameters by an extended Perrin equation for restricted rotation of fluorophores.
Authorsvan der Meer BW, van Hoeven RP, van Blitterswijk WJ
JournalBiochim Biophys Acta
PubMed ID3942718
'An extended Perrin equation is derived applicable to the restricted rotation of fluorophores. The equation results in a relation between time-resolved (r infinity) and steady-state fluorescence anisotropy (rs) data. This relation contains a parameter m, which expresses the difference between rotational diffusion in a lipid membrane and that in an ... More
Interaction of amphotericin B and its N-fructosyl derivative with murine thymocytes: a comparative study using fluorescent membrane probes.
AuthorsHenry-Toulmé N, Seman M, Bolard J
JournalBiochim Biophys Acta
PubMed ID2787669
'The polyene antibiotics amphotericin B (AmB) and N-(1-deoxy-D-fructos-1-yl)amphotericin (N-Fru-AmB) have different activity towards murine thymocytes (N-Fru-AmB is less toxic but is a potent immunomodulator). The interactions of the drugs with these cells have been studied by fluorescence methods. Fluorescence energy transfer from 1-[4-(trimethylammonio) phenyl]-6-phenylhexa-1,3,5-triene, p-toluenesulfonate (TMA-DPH) to polyenes was used ... More
Relationship between vasopressin-sensitive water transport and plasma membrane fluidity in kidney collecting tubule.
AuthorsFushimi K, Verkman AS
JournalAm J Physiol
PubMed ID1987773
'The role of plasma membrane fluidity in the regulation of kidney tubule water permeability has been uncertain. We have used new methods to image the fluorescence anisotropy of fluidity-sensitive fluorophores (Fushimi, Dix, and Verkman. Biophys. J. 57: 241-254, 1990) to quantitate membrane fluidity in cells of the vasopressin-sensitive cortical collecting ... More
Effect of the fungicides tributyltin acetate and tributyltin chloride on multilamellar liposomes: fluorescence studies.
AuthorsAmbrosini A, Bertoli E, Tanfani F, Zolese G
JournalChem Phys Lipids
PubMed ID1742811
'The influence of tri-n-butyltin acetate (TBTA) and tri-n-butyltin chloride (TBTC) on the physico-chemical state of charged and neutral phospholipids was investigated using multilamellar liposomes. The thermal dependence of steady state fluorescence polarization of DPH and its charged derivative TMA-DPH was recorded. The two fungicides lowered DPPC phase transition temperature and ... More
Partial activation of the factor VIIIa-factor IXa enzyme complex by dihexanoic phosphatidylserine at submicellar concentrations.
AuthorsGilbert GE, Arena AA
JournalBiochemistry
PubMed ID9271508
'Phosphatidylserine (PS)-containing membranes increase the kcat of the factor VIIIa-factor IXa enzyme complex by more than 1000-fold. While PS supports specific, high-affinity membrane binding of factor VIIIa and factor IXa, it is not known whether PS is the lipid that activates the membrane-bound complex. It is also not known whether ... More
Evidence that cholesteryl ester transfer protein-mediated reductions in reconstituted high density lipoprotein size involve particle fusion.
AuthorsRye KA, Hime NJ, Barter PJ
JournalJ Biol Chem
PubMed ID9020099
'It is well established that cholesteryl ester transfer protein (CETP) changes the size of high density lipoproteins (HDL) during incubation in vitro. It has been suggested that HDL.CETP.HDL ternary complex formation is involved in these changes. The present results, which are consistent with CETP changing the size of spherical reconstituted ... More
NMR studies of the relationship between the changes of membrane lipids and the cisplatin-resistance of A549/DDP cells.
AuthorsHuang Z, Tong Y, Wang J, Huang Y
JournalCancer Cell Int
PubMed ID12718757
'Changes of membrane lipids in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP cells during the apoptotic process induced by a clinical dose of cisplatin (30 &mgr;M) were detected by 1H and 31P-NMR spectroscopy and by membrane fluidity measurement. The apoptotic phenotypes of the two cell lines were monitored with flow cytometry. The ... More
Probing membrane alterations associated with anthracycline resistance using fluorescent dyes.
AuthorsKessel D
JournalBiochem Pharmacol
PubMed ID3196354
'Properties of membranes of intact P388 murine lymphoblastic leukemia and a sub-line selected for resistance to adriamycin (P388/ADR) were examined using two fluorescent probes: diphenylhexatriene (DPH) and trimethylammonium diphenylhexatriene (T-DPH). Time-dependent changes in dye accumulation, fluorescence anisotropy, and lifetimes were measured. Accumulation of DPH, which eventually labels all cellular lipids, ... More
Erythrocyte, platelet and polymorphonuclear leukocyte membrane dynamic properties in essential hypertension.
AuthorsCaimi G
JournalClin Hemorheol Microcirc
PubMed ID9356784
'In subjects with essential hypertension we evaluated, respectively, the red cell membrane protein lateral mobility (obtained marking intact red blood cells with pyrene-3-maleimide (3-PM)), the erythrocyte membrane fluidity (obtained marking intact erythrocytes with 10-(1-pyrene) decanoic acid), the red cell membrane transverse fluidity gradient (obtained marking intact red blood cells with ... More
Alterations in erythrocyte membrane fluidity in children with trisomy 21: a fluorescence study.
AuthorsKantar A, Giorgi PL, Curatola G, Fiorini R
JournalBiol Cell
PubMed ID1393150
'Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemoglobin-free erythrocyte membranes. Our results demonstrate a significant decrease in DPH fluorescence anisotropy and a significant increase ... More
Cypermethrin-induced plasma membrane perturbation on erythrocytes from rats: reduction of fluidity in the hydrophobic core and in glutathione peroxidase activity.
AuthorsGabbianelli R, Falcioni G, Nasuti C, Cantalamessa F
JournalToxicology
PubMed ID12049839
'The effects of treatment with the synthetic insecticide cypermethrin on plasma membrane fluidity, lipid peroxidation and antioxidant status in rat erythrocytes were investigated. Rats were treated by gavage with a low dose (12.5 mg/kg body weight per day) of cypermethrin in corn oil for 60 days. DPH and TMA-DPH fluorescence ... More
A mechanism for stabilization of membranes at low temperatures by an antifreeze protein.
AuthorsTomczak MM, Hincha DK, Estrada SD, Wolkers WF, Crowe LM, Feeney RE, Tablin F, Crowe JH
JournalBiophys J
PubMed ID11806929
'Polar fish, cold hardy plants, and overwintering insects produce antifreeze proteins (AFPs), which lower the freezing point of solutions noncolligatively and inhibit ice crystal growth. Fish AFPs have been shown to stabilize membranes and cells in vitro during hypothermic storage, probably by interacting with the plasma membrane, but the mechanism ... More
Membrane fluidity aspects in endocytosis; a study with the fluorescent probe trimethylamino-diphenylhexatriene in L929 cells.
AuthorsIllinger D, Poindron P, Kuhry JG
JournalBiol Cell
PubMed ID1933025
'The fluorescent hydrophobic plasma membrane probe, trimethylamino-diphenylhexatriene (TMA-DPH) was previously shown to follow the plasma membrane throughout its internalization and recycling process and thus to behave as a marker for endo- and exocytosis in living cell systems. In this paper, we made use of these properties to investigate membrane fluidity ... More
Methyl-beta-cyclodextrins and liposomes as water-soluble carriers for cholesterol incorporation into membranes and its evaluation by a microenzymatic fluorescence assay and membrane fluidity-sensitive dyes.
AuthorsHärtel S, Diehl HA, Ojeda F
JournalAnal Biochem
PubMed ID9570841
'A variety of methods to incorporate cholesterol into lipid membrane systems have been applied with varying success. We tested an incorporation method based on cholesterol-loaded methyl-beta-cyclodextrins and compared it to a method that uses cholesterol-loaded liposomes. With methyl-beta-cyclodextrin, we increased the cholesterol content in microsomal membranes to almost the fourfold ... More
Reconstitution of the leucine transport system of Lactococcus lactis into liposomes composed of membrane-spanning lipids from Sulfolobus acidocaldarius.
'The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium Lactococcus lactis was investigated. Liposomes were prepared from mixtures of monolayer lipids and the bilayer lipid phosphatidylcholine (PC), analyzed on their miscibility, and fused with membrane ... More
Interaction of rifampicin and isoniazid with large unilamellar liposomes: spectroscopic location studies.
AuthorsRodrigues C, Gameiro P, Prieto M, de Castro B
JournalBiochim Biophys Acta
PubMed ID12595084
'The location of isoniazid and rifampicin, two tuberculostatics commonly used for the treatment of Mycobacterium tuberculosis and Mycobacterium avium complex infectious diseases, in bilayers of dimyristoyl-L-alpha-phosphatidylcholine (DMPC) and dimyristoyl-L-a-phosphatidylglycerol (DMPG) have been studied by 1H NMR and fluorimetric methods. Steady-state fluorescence intensity and fluorescence energy transfer studies between rifampicin and ... More
Contrary results on mouse hepatitis virus type 3 susceptibility in A/J mouse hepatocytes of phosphatidylserine treatment and of a hypercholesterolaemic diet: no correlation with membrane fluidity levels.
AuthorsNonnenmacher H, Illinger D, Kuhry JG, Kirn A, Braunwald J
JournalBiochem Biophys Res Commun
PubMed ID7980608
'The aim of this study was to examine whether or not membrane fluidity directly influences infection by enveloped viruses, and, more precisely here, the susceptibility of A/J mouse hepatocytes to Mouse Hepatitis Virus type 3 (MHV3). We therefore studied, in parallel, the effects on hepatocyte membrane fluidity and on intracellular ... More
Influence of the redox state of ubiquinones and plastoquinones on the order of lipid bilayers studied by fluorescence anisotropy of diphenylhexatriene and trimethylammonium diphenylhexatriene.
AuthorsSkowronek M, Jemiola-Rzeminska M, Kruk J, Strzalka K
JournalBiochim Biophys Acta
PubMed ID8634304
'The measurements of diphenylhexatriene (DPH) and trimethylammonium diphenylhexatriene (TMA-DPH) fluorescence anisotropy in egg yolk lecithin (EYL) and of DPH anisotropy in dipalmitoylphosphatidylcholine (DPPC) liposomes containing different concentrations of oxidized and reduced ubiquinone (UQ) and plastoquinone (PQ) homologues have been performed. All the oxidized UQ homologues strongly induced ordering of EYL ... More
Pyrazine derivatives affect membrane fluidity of vascular smooth muscle microsomes in relation to their biological activity.
AuthorsNie SQ, Kwan CY, Epand RM
JournalEur J Pharmacol
PubMed ID8380557
'We have previously demonstrated that several structurally similar pyrazine derivatives, tetramethylpyrazine (TMP), triethylpyrazine (3EP) and tetraethylpyrazine (TEP) consistently inhibited a wide spectrum of plasmalemma-associated biological activities of vascular smooth muscles in contractility and radioligand binding studies in the following order of increasing potency, TMP < 3EP < TEP. We speculated ... More
Identification of syntenin as a protein of the apical early endocytic compartment in Madin-Darby canine kidney cells.
AuthorsFialka I, Steinlein P, Ahorn H, Böck G, Burbelo PD, Haberfellner M, Lottspeich F, Paiha K, Pasquali C, Huber LA
JournalJ Biol Chem
PubMed ID10473577
'We used flow cytometry to sort and analyze apical and basolateral endocytic vesicles from filter-grown Madin-Darby canine kidney (MDCK) cells after membrane internalization of the lipophilic fluorescent probe trimethylamino-diphenylhexatriene. Western blot analysis of sorted fractions showed enrichment of the early endosomal markers transferrin receptor and the small GTPase Rab5. Two-dimensional ... More
Parallel investigation of exocytosis kinetics and membrane fluidity changes in human platelets with the fluorescent probe, trimethylammonio-diphenylhexatriene.
AuthorsKubina M, Lanza F, Cazenave JP, Laustriat G, Kuhry JG
JournalBiochim Biophys Acta
PubMed ID3593721
'A simple, flexible and sensitive fluorescence method is described, which, from the same experiment, provides coupled quantitative informations on membrane fluidity changes and exocytosis, and reliable kinetic analyses of these effects, in intact cell suspensions. The method is based on the features peculiar to trimethylammonio-diphenylhexatriene (TMA-DPH), a fluorescent hydrophobic probe, ... More
An alternative to digoxigenin-labeled primers for manual nonradioactive sequencing allows reading of more than 700 bases.
The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function.
AuthorsStubbs CD, Smith AD
JournalBiochim Biophys Acta
PubMed ID6229284
Lipids and their effects on membrane proteins: evidence against a role for fluidity.
AuthorsLee AG
JournalProg Lipid Res
PubMed ID1816552
Fluorescence analysis of lipoprotein peroxidation.
AuthorsDousset N, Ferretti G, Taus M, Valdiguiè P, Curatola G
JournalMethods Enzymol
PubMed ID8015481
Flow cytometric analyses and sorting of neural cells for transplantation.
AuthorsNotter MF, Gash DM, Leary JF
JournalProg Brain Res
PubMed ID3247456
Spectroscopic techniques in the study of membrane solubilization, reconstitution and permeabilization by detergents.
AuthorsGoñi FM, Alonso A
JournalBiochim Biophys Acta
PubMed ID11090818
This review focuses on the use of spectroscopic techniques for the study of membrane solubilization, reconstitution, and permeabilization by detergents. Turbidity and light scattering, visible and infrared spectroscopic methods, fluorescence, nuclear magnetic resonance, electron spin resonance and X-ray diffraction are examined from the point of view of their applicability to ... More
Effect of PAF on erythrocyte membrane heterogeneity: a fluorescence study.
AuthorsKantar A, Giorgi PL, Curatola G, Fiorini R
JournalAgents Actions
PubMed ID1862752
The effect of platelet activating factor, PAF, on the erythrocyte membrane heterogeneity was investigated by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemoglobin-free erythrocyte membranes. PAF induced a significant increase of the lipid order in the exterior part of the membrane and a decrease ... More
Dynamic fluorescence measurements on the main phase transition of dipalmytoylphosphatidylcholine vesicles.
AuthorsGenz A, Holzwarth JF
JournalEur Biophys J
PubMed ID3757928
The kinetics of the main phase transition in dipalmytoylphosphatidylcholine (DPPC) vesicles have been investigated using our iodine laser-T-jump technique with fluorescence detection. A set of three fluorescent probes has been used to sense different parts of the bilayer hydrocarbon chain region. The well established membrane probes DPH and TMADPH as ... More
Increase of the fluidity of the lipid bilayer of the inner mitochondrial membrane by succinate and phenylsuccinate: a study by EPR and fluorescence.
AuthorsMutet C, Duportail G, Crémel G, Waksman A
JournalBiochem Biophys Res Commun
PubMed ID6324784
Electron paramagnetic resonance and fluorescence experiments have demonstrated that the lipid matrix of inner membrane of mitochondria was more fluid than the control membrane when incubated with succinate or with one of its non permeant and non metabolizable analog, phenylsuccinate, both of which induce a protein movement from the inner ... More
Effect of PAF on human lymphocyte membranes: a fluorescence study.
AuthorsKantar A, Giorgi PL, Fiorini R
JournalAgents Actions
PubMed ID8317302
We have studied the effect of platelet-activating factor (PAF) on the physico-chemical organization of human lymphocyte plasma membranes by measuring the steady-state fluorescence anisotropy and the fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in lymphocyte plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior ... More
Influence of the clathrin coat on the membrane lipidic organization of endocytic vesicles: a fluorescence study.
AuthorsLiaubet A, Egret-Charlier M, Kuhry JG
JournalBiochim Biophys Acta
PubMed ID7918559
Endocytic coated vesicles (CV) were purified from bovine brain, and uncoated vesicles (UV) were obtained from the latter by dialysis against 1 M Tris. Membrane dynamics were explored in both vesicle populations using two complementary fluorescence approaches: diphenylhexatriene fluorescence anisotropy to account for rotational lipid movements, and pyrene excimerization with ... More