Head regeneration in wild-type hydra requires de novo neurogenesis.
AuthorsMiljkovic-Licina M, Chera S, Ghila L, Galliot B
JournalDevelopment
PubMed ID17301084
'Because head regeneration occurs in nerve-free hydra mutants, neurogenesis was regarded as dispensable for this process. Here, in wild-type hydra, we tested the function of the ParaHox gsx homolog gene, cnox-2, which is a specific marker for bipotent neuronal progenitors, expressed in cycling interstitial cells that give rise to apical ... More
On-chip, cell-based microarray immunofluorescence assay for high-throughput analysis of target proteins.
AuthorsFernandes TG, Kwon SJ, Lee MY, Clark DS, Cabral JM, Dordick JS,
JournalAnal Chem
PubMed ID18656951
'We have developed an immunofluorescence-based assay for high-throughput analysis of target proteins on a three-dimensional cellular microarray platform. This process integrates the use of three-dimensional cellular microarrays, which should better mimic the cellular microenvironment, with sensitive immunofluorescence detection and provides quantitative information on cell function. To demonstrate this assay platform, ... More
YKL-40 is differentially expressed in human embryonic stem cells and in cell progeny of the three germ layers.
AuthorsBrøchner CB, Johansen JS, Larsen LA, Bak M, Mikkelsen HB, Byskov AG, Andersen CY, Møllgård K,
JournalJ Histochem Cytochem
PubMed ID22140133
'The secreted glycoprotein YKL-40 participates in cell differentiation, inflammation, and cancer progression. High YKL-40 expression is reported during early human development, but its functions are unknown. Six human embryonic stem cell (hESC) lines were cultured in an atmosphere of low or high oxygen tension, in culture medium with or without ... More
Regulation of p27Kip1 by Sox2 maintains quiescence of inner pillar cells in the murine auditory sensory epithelium.
AuthorsLiu Z, Walters BJ, Owen T, Brimble MA, Steigelman KA, Zhang L, Mellado Lagarde MM, Valentine MB, Yu Y, Cox BC, Zuo J,
JournalJ Neurosci
PubMed ID22855803
Sox2 plays critical roles in cell fate specification during development and in stem cell formation; however, its role in postmitotic cells is largely unknown. Sox2 is highly expressed in supporting cells (SCs) of the postnatal mammalian auditory sensory epithelium, which unlike non-mammalian vertebrates remains quiescent even after sensory hair cell ... More
Improvement of combined FISH and immunofluorescence to trace the fate of somatic stem cells after transplantation.
AuthorsDonadoni C, Corti S, Locatelli F, Papadimitriou D, Guglieri M, Strazzer S, Bossolasco P, Salani S, Comi GP
JournalJ Histochem Cytochem
PubMed ID15385579
Fluorescence in situ hybridization (FISH) combined with immunohistochemistry of tissue-specific markers provides a reliable method for characterizing the fate of somatic stem cells in transplantation experiments. Furthermore, the association between FISH and fluorescent gene reporter detection can unravel cell fusion phenomena, which could account for apparent transdifferentiation events. However, despite ... More