'Hereditary spastic paraplegia (HSP) is a neurodegenerative disorder that is characterized by retrograde axonal degeneration that primarily affects long spinal neurons. The disease is clinically heterogeneous, and there are >20 genetic loci identified. Here, we show a physical interaction between spastin and atlastin, two autosomal dominant HSP gene products. Spastin ... More
On-chip, cell-based microarray immunofluorescence assay for high-throughput analysis of target proteins.
AuthorsFernandes TG, Kwon SJ, Lee MY, Clark DS, Cabral JM, Dordick JS,
JournalAnal Chem
PubMed ID18656951
'We have developed an immunofluorescence-based assay for high-throughput analysis of target proteins on a three-dimensional cellular microarray platform. This process integrates the use of three-dimensional cellular microarrays, which should better mimic the cellular microenvironment, with sensitive immunofluorescence detection and provides quantitative information on cell function. To demonstrate this assay platform, ... More
Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain.
AuthorsWang Y, Kaneko N, Asai N, Enomoto A, Isotani-Sakakibara M, Kato T, Asai M, Murakumo Y, Ota H, Hikita T, Namba T, Kuroda K, Kaibuchi K, Ming GL, Song H, Sawamoto K, Takahashi M,
JournalJ Neurosci
PubMed ID21632933
In postnatally developing and adult brains, interneurons of the olfactory bulb (OB) are continuously generated at the subventricular zone of the forebrain. The newborn neuroblasts migrate tangentially to the OB through a well defined pathway, the rostral migratory stream (RMS), where the neuroblasts undergo collective migration termed ... More
In vivo circadian function of casein kinase 2 phosphorylation sites in Drosophila PERIOD.
AuthorsLin JM, Schroeder A, Allada R,
JournalJ Neurosci
PubMed ID16319317
Phosphorylation plays a key role in the precise timing of circadian clocks. Daily rhythms of phosphorylation of the Drosophila circadian clock component PERIOD (PER) were first described more than a decade ago, yet little is known about their phosphorylation sites and their function in circadian behavior. Here we show that ... More
A novel connection between rods and ON cone bipolar cells revealed by ectopic metabotropic glutamate receptor 7 (mGluR7) in mGluR6-deficient mouse retinas.
AuthorsTsukamoto Y, Morigiwa K, Ishii M, Takao M, Iwatsuki K, Nakanishi S, Fukuda Y,
JournalJ Neurosci
PubMed ID17553999
Since the discovery of direct chemical synapses between rod photoreceptor and OFF cone bipolar cells in mouse retinas, whether the ON cone bipolar cell also receive direct chemical input from rod has been a pending question. In finding that metabotropic glutamate receptor 7 (mGluR7) was uniquely expressed in dendrites of ... More