We defined the function of type I interferons (IFNs) in defense against reovirus strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression of mRNA for IFN-alpha, IFN-beta, and Mx-1 in PPs and caused localized intestinal ... More
The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma.
AuthorsMori K, Itoi M, Tsukamoto N, Kubo H, Amagai T,
JournalInt Immunol
PubMed ID17493961
'It is known that selected populations of lymphoid cells migrate into and from the adult thymus through blood vessels at the cortico-medullary junction and in the medulla. Here, we show that in the perivascular spaces (PVS) of mice surrounding large blood vessels, CD117-positive hematopoietic progenitor cells, CD4 single-positive (SP) and ... More
Mesenchymal cells are required for functional development of thymic epithelial cells.
AuthorsItoi M, Tsukamoto N, Yoshida H, Amagai T,
JournalInt Immunol
PubMed ID17625108
'Epithelial-mesenchymal interactions have essential roles in thymus organogenesis. Mesenchymal cells are known to be required for epithelial cell proliferation. However, the contribution of mesenchymal cells to thymic epithelial cell differentiation is still unclear. In the present study, we have investigated the roles of mesenchymal cells in functional development of epithelial ... More
A novel population of myeloid cells responding to coxsackievirus infection assists in the dissemination of virus within the neonatal CNS.
AuthorsTabor-Godwin JM, Ruller CM, Bagalso N, An N, Pagarigan RR, Harkins S, Gilbert PE, Kiosses WB, Gude NA, Cornell CT, Doran KS, Sussman MA, Whitton JL, Feuer R,
JournalJ Neurosci
PubMed ID20573913
'Enterovirus infection in newborn infants is a significant cause of aseptic meningitis and encephalitis. Using a neonatal mouse model, we previously determined that coxsackievirus B3 (CVB3) preferentially targets proliferating neural stem cells located in the subventricular zone within 24 h after infection. At later time points, immature neuroblasts, and eventually ... More
Effective fiber hypertrophy in satellite cell-depleted skeletal muscle.
AuthorsMcCarthy JJ, Mula J, Miyazaki M, Erfani R, Garrison K, Farooqui AB, Srikuea R, Lawson BA, Grimes B, Keller C, Van Zant G, Campbell KS, Esser KA, Dupont-Versteegden EE, Peterson CA,
JournalDevelopment
PubMed ID21828094
'An important unresolved question in skeletal muscle plasticity is whether satellite cells are necessary for muscle fiber hypertrophy. To address this issue, a novel mouse strain (Pax7-DTA) was created which enabled the conditional ablation of >90% of satellite cells in mature skeletal muscle following tamoxifen administration. To test the hypothesis ... More
Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species.
AuthorsTóth ZE, Mezey E,
JournalJ Histochem Cytochem
PubMed ID17242468
After immunohistochemistry (IHC) began to be used routinely, a number of investigators worked on methods for staining multiple molecules in the same tissue sections or cells. Achieving this goal was not easy, however. One reason for this is that the majority of primary antibodies used in IHC reactions are raised ... More
Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing.
AuthorsToth ZE, Shahar T, Leker R, Szalayova I, Bratincsák A, Key S, Lonyai A, Németh K, Mezey E,
JournalExp Cell Res
PubMed ID17428464
The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in ... More