Multiplex detection of RNA expression in Drosophila embryos.
AuthorsKosman D, Mizutani CM, Lemons D, Cox WG, McGinnis W, Bier E
JournalScience
PubMed ID15297669
We present a fluorescence-based, multiplex in situ hybridization method that permits the simultaneous detection of five differently labeled antisense RNA probes and up to seven differ-ent transcripts in a single Drosophila embryo. We also show that it should be possible to increase the number of detected transcripts substantially with nascent ... More
Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing.
AuthorsToth ZE, Shahar T, Leker R, Szalayova I, Bratincsák A, Key S, Lonyai A, Németh K, Mezey E,
JournalExp Cell Res
PubMed ID17428464
The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in ... More