Dynamic resolution of acrosomal exocytosis in human sperm.
AuthorsHarper CV, Cummerson JA, White MR, Publicover SJ, Johnson PM,
JournalJ Cell Sci
PubMed ID18522990
An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona ... More
Real-time observations of individual macaque sperm undergoing tight binding and the acrosome reaction on the zona pellucida.
Changes in binding affinity, acrosomal status, and motility of living sperm on the zona pellucida were for the first time in any mammalian species directly observed and analyzed with video microscopy. A single zona was air-dried and rehydrated on a microscope slide, and a coverslip supported by glass beads was ... More
Soybean trypsin inhibitor as a probe for the acrosome reaction in motile cynomolgus macaque sperm.
Soybean trypsin inhibitor (SBTI) inhibits the catalytic activity of serine proteases, and has been shown to bind to acrosin, an acrosomal hydrolase which is not exposed on the surface of macaque sperm until after the acrosome reaction. Following activation with caffeine and dibutyryl cAMP, cynomolgus macaque sperm were induced to ... More