Receptor and membrane recycling can occur with unaltered efficiency despite dramatic Rab5(q79l)-induced changes in endosome geometry.
AuthorsCeresa BP, Lotscher M, Schmid SL
JournalJ Biol Chem
PubMed ID11136733
'Current models for sorting in the endosomal compartment suggest that endosomal geometry plays a significant role as membrane-bound proteins accumulate in tubular regions for recycling, and lumenal markers accumulate in large vacuolar portions for delivery to lysosomes. Rab5, a small molecular weight GTPase, functions in the formation and maintenance of ... More
Successful transfection of biotinylated beta-galactosidase gene conjugated with transferrin into leukemia cells and interleukin-2-stimulated lymphocytes via transferrin receptor.
AuthorsNeda H, Takahashi M, Itoh Y, Koshita Y, Matsuyama T, Watanabe N, Kohgo Y, Niitsu Y
JournalAnn N Y Acad Sci
PubMed ID8024208
Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts.
AuthorsEhehalt R, Keller P, Haass C, Thiele C, Simons K
JournalJ Cell Biol
PubMed ID12515826
'Formation of senile plaques containing the beta-amyloid peptide (A beta) derived from the amyloid precursor protein (APP) is an invariant feature of Alzheimer''s disease (AD). APP is cleaved either by beta-secretase or by alpha-secretase to initiate amyloidogenic (release of A beta) or nonamyloidogenic processing of APP, respectively. A key to ... More
Iron acquisition in Pasteurella haemolytica: expression and identification of a bovine-specific transferrin receptor.
AuthorsOgunnariwo JA, Schryvers AB
JournalInfect Immun
PubMed ID2365453
'Seven type 1 field isolates of Pasteurella haemolytica were screened for their ability to use different transferrins as a source of iron for growth. All seven strains were capable of using bovine but not human, porcine, avian, or equine transferrin. A screening assay failed to detect siderophore production in any ... More
Isolation of bright aggregate fluctuations in a multipopulation image correlation spectroscopy system using intensity subtraction.
AuthorsRocheleau JV, Wiseman PW, Petersen NO
JournalBiophys J
PubMed ID12770905
'Image correlation spectroscopy allows sensitive measurement of the spatial distribution and aggregation state of fluorescent membrane macro molecules. When studying a single population system (i.e., aggregates of similar brightness), an accurate measure can be made of the aggregate number per observation area, but this measurement becomes much more complex in ... More
Dynamin:GTP controls the formation of constricted coated pits, the rate limiting step in clathrin-mediated endocytosis.
AuthorsSever S, Damke H, Schmid SL
JournalJ Cell Biol
PubMed ID10974001
'The GTPase dynamin is essential for receptor-mediated endocytosis, but its function remains controversial. A domain of dynamin, termed the GTPase effector domain (GED), controls dynamin''s high stimulated rates of GTP hydrolysis by functioning as an assembly-dependent GAP. Dyn(K694A) and dyn(R725A) carry point mutations within GED resulting in reduced assembly stimulated ... More
Further studies on targeted DNA transfer to cells using a highly efficient delivery system of biotinylated transferrin and biotinylated polylysine complexed to streptavidin.
AuthorsSchoeman R, Joubert D, Ariatti M, Hawtrey AO
JournalJ Drug Target
PubMed ID7773613
'Conjugates consisting of biotinylated transferrin and biotinylated poly-L-lysine attached to streptavidin have been prepared and found to transfer luciferase plasmid DNA very efficiently to HeLa cells in the presence of chloroquine. Transfection was dependent on (i) use of biotinylated short chain polylysine containing 70 lysine residues, (ii) biotinylated transferrin containing ... More
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
AuthorsXiao W, Warrington KH, Hearing P, Hughes J, Muzyczka N
JournalJ Virol
PubMed ID12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from ... More
The use of transferrin for enrichment of fetal cells from maternal blood.
AuthorsSerlachius M, Von Koskull H, Wessman M, Schröder J
JournalPrenat Diagn
PubMed ID10820410
'Iron loaded transferrin (holotransferrin) was used for enrichment of fetal cells from peripheral blood of pregnant women. Cord blood samples were used to evaluate enrichment efficacy of single and double MACS separations. Blood samples were obtained from 10 pregnant women prior to chorion villus sampling (CVS). Erythroblasts and other mononuclear ... More
EphrinB-EphB signalling regulates clathrin-mediated endocytosis through tyrosine phosphorylation of synaptojanin 1.
AuthorsIrie F, Okuno M, Pasquale EB, Yamaguchi Y
JournalNat Cell Biol
PubMed ID15821731
'Recent studies show that Eph receptors act mainly through the regulation of actin reorganization. Here, we show a novel mode of action for EphB receptors. We identify synaptojanin 1 - a phosphatidylinositol 5''-phosphatase that is involved in clathrin-mediated endocytosis - as a physiological substrate for EphB2. EphB2 causes tyrosine phosphorylation ... More
Molecular cloning of transferrin receptor 2. A new member of the transferrin receptor-like family.
AuthorsKawabata H, Yang R, Hirama T, Vuong PT, Kawano S, Gombart AF, Koeffler HP
JournalJ Biol Chem
PubMed ID10409623
'Transferrin receptor (TfR) plays a major role in cellular iron uptake through binding and internalizing a carrier protein transferrin (Tf). We have cloned, sequenced, and mapped a human gene homologous to TfR, termed TfR2. Two transcripts were expressed from this gene: alpha (approximately 2.9 kilobase pairs), and beta (approximately 2.5 ... More
Transferrinfection: a highly efficient way to express gene constructs in eukaryotic cells.
AuthorsZatloukal K, Wagner E, Cotten M, Phillips S, Plank C, Steinlein P, Curiel DT, Birnstiel ML
JournalAnn N Y Acad Sci
PubMed ID1340117
Identification and characterization of a porcine-specific transferrin receptor in Actinobacillus pleuropneumoniae.
AuthorsGonzalez GC, Caamano DL, Schryvers AB
JournalMol Microbiol
PubMed ID2233254
All six strains of Actinobacillus pleuropneumoniae screened for the ability to use different transferrins as a source of iron for growth were capable of using porcine but not human, bovine, or avian transferrins. A specific binding activity for porcine transferrin (pTf) was expressed in cells grown in the presence of ... More
Isolation and identification of a putative porcine transferrin receptor from Actinobacillus pleuropneumoniae biotype 1.
AuthorsRicard MA, Archibald FS, Niven DF
JournalJ Gen Microbiol
PubMed ID1791429
Each of two affinity isolation methods, the first based on biotinylated porcine transferrin plus streptavidin-agarose, and the second on Sepharose-coupled porcine transferrin, followed by SDS-PAGE, allowed the isolation and identification of two potential porcine-transferrin-binding polypeptides (approximately 64 kDa and 99 kDa) from total membranes of Actinobacillus pleuropneumoniae grown under iron-restricted ... More
Contact-dependent acquisition of transferrin-bound iron by two strains of Haemophilus parasuis.
AuthorsCharland N, D'Silva CG, Dumont RA, Niven DF
JournalCan J Microbiol
PubMed ID7728656
Two strains of Haemophilus parasuis, namely, the type strain (ATCC 19417) and strain E751, were investigated with respect to iron acquisition. Both strains produced iron-repressible outer membrane proteins and could acquire iron from porcine transferrin but not from porcine lactoferrin. Neither strain used bovine transferrin, and human transferrin was used ... More
Endocytosis and intracellular transport of transferrin across the lactating rabbit mammary epithelial cell.
AuthorsSeddiki T, Delpal S, Ollivier-Bousquet M
JournalJ Histochem Cytochem
PubMed ID1527373
To study the transcytosis and segregation of ligand in the mammary epithelial cell, endocytosis and intracellular transit of human blood transferrin were followed in lactating rabbit mammary epithelial cells. Human transferrin labeled with biotin added to an incubation medium was bound to the basal membrane of mammary epithelial cells and ... More
Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology.
AuthorsMorag E, Bayer EA, Wilchek M
JournalAnal Biochem
PubMed ID8954558
Chemically modified forms of egg-white avidin and bacterial streptavidin (termed nitro-avidin and nitro-streptavidin, respectively), in which the binding-site tyrosine was nitrated, were used for several biotechnological applications. The fundamental difference between nitro-avidin and the native protein is that interaction of the modified protein with biotin can be reversed under relatively ... More
Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli.
AuthorsNardone E, Rosano C, Santambrogio P, Curnis F, Corti A, Magni F, Siccardi AG, Paganelli G, Losso R, Apreda B, Bolognesi M, Sidoli A, Arosio P
JournalEur J Biochem
PubMed ID9760187
The mature hen avidin encoded by a synthetic cDNA was expressed in Escherichia coli in an insoluble form. After resolubilization, renaturation and purification, a recovery of about 20 mg/l cell culture was obtained. ELISA assays indicated no apparent differences in biotin binding between the natural and recombinant avidins. In addition, ... More
Copper-stimulated endocytosis and degradation of the human copper transporter, hCtr1.
AuthorsPetris MJ, Smith K, Lee J, Thiele DJ
JournalJ Biol Chem
PubMed ID12501239
Copper uptake at the plasma membrane and subsequent delivery to copper-dependent enzymes is essential for many cellular processes, including mitochondrial oxidative phosphorylation, free radical detoxification, pigmentation, neurotransmitter synthesis, and iron metabolism. However, intracellular levels of this nutrient must be controlled because it is potentially toxic in excess concentrations. The hCtr1 ... More
Studies on the transfer of DNA into cells through use of avidin-polylysine conjugates complexed to biotinylated transferrin and DNA.
AuthorsStrydom S, Van Jaarsveld P, Van Helden E, Ariatti M, Hawtrey A
JournalJ Drug Target
PubMed ID8069555
A simple procedure has been developed for studying the transfer of DNA into cells using the process of receptor-mediated endocytosis. Poly-L-lysine 460 was covalently attached to the carbohydrate chains of avidin via periodate oxidation and NaBH4 reduction to give avidin-pLys460. Following purification through Sephacryl S-300, the conjugate was reacted with ... More
Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11.
AuthorsSönnichsen B, De Renzis S, Nielsen E, Rietdorf J, Zerial M
JournalJ Cell Biol
PubMed ID10811830
Two endosome populations involved in recycling of membranes and receptors to the plasma membrane have been described, the early and the recycling endosome. However, this distinction is mainly based on the flow of cargo molecules and the spatial distribution of these membranes within the cell. To get insights into the ... More
Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.
AuthorsConner SD, Schmid SL
JournalJ Cell Biol
PubMed ID11877461
The mu 2 subunit of the AP2 complex is known to be phosphorylated in vitro by a copurifying kinase, and it has been demonstrated recently that mu 2 phosphorylation is required for transferrin endocytosis (Olusanya, O., P.D. Andrews, J.R. Swedlow, and E. Smythe. 2001. Curr. Biol. 11:896-900). However, the identity ... More
Rabphilin localizes with the cell actin cytoskeleton and stimulates association of granules with F-actin cross-linked by {alpha}-actinin.
In endocrine cell, granules accumulate within an F-actin-rich region below the plasma membrane. The mechanisms involved in this process are largely unknown. Rabphilin is a cytosolic protein that is expressed in neurons and neuroendocrine cells and binds with high affinity to members of the Rab3 family of GTPases localized to ... More
Comparative study of iron acquisition by biotype 1 and biotype 2 strains of Actinobacillus pleuropneumoniae.
AuthorsD'Silva CG, Archibald FS, Niven DF
JournalVet Microbiol
PubMed ID7667902
Four strains of the swine pathogen, Actinobacillus pleuropneumoniae, namely, the type strain (ATCC 27088; biotype 1), the 'reference' strain of biotype 2 (Bertschinger 2008/76), and two additional biotype 1 strains, strain BC181, which is less virulent than the type strain, and strain K17, which was isolated from a lamb, were ... More
The sorting and segregation mechanism of the endocytic pathway is functional in a cell-free system.
AuthorsWessling-Resnick M, Braell WA
JournalJ Biol Chem
PubMed ID2153110
A cell-free system which reconstitutes early stages of receptor-mediated endocytosis has been developed, based on detection of the association between avidin-beta-galactosidase (Av beta Gal) and biotin-transferrin (B-Tf). Initially, Av beta Gal (a fluid-phase marker) and B-Tf (receptor-bound) are internalized and delivered to a common endosomal compartment in vivo and in ... More
Affinity cleavage and targeted catalysis of proteins using the avidin-biotin system.
AuthorsBayer EA, Grootjans JJ, Alon R, Wilchek M
JournalBiochemistry
PubMed ID2271711
The avidin-biotin system was used in order to target enzymes to their substrates in complex mixtures of proteins in solution. The approach described here thus mimics natural systems in which enzymes usually act in selective fashion, due, perhaps, to proximity effects. For affinity cleavage studies, biotinyl transferrin was used as ... More
Utilization of transferrin and salmon serum as sources of iron by typical and atypical strains of Aeromonas salmonicida.
AuthorsHirst ID, Ellis AE
JournalMicrobiology
PubMed ID8704995
The ability of typical and atypical strains of Aeromonas salmonicida to utilize non-haem sources of protein-bound iron was evaluated. (i) In a plate bioassay, the suppression of growth imposed on typical and atypical A. salmonicida by addition of the high-affinity iron chelator ethylenediamine-di(o- hydroxyphenylacetic acid) (EDDA) to the growth medium ... More
Imaging of endosome fusion in BHK fibroblasts based on a novel fluorimetric avidin-biotin binding assay.
AuthorsEmans N, Biwersi J, Verkman AS
JournalBiophys J
PubMed ID8527685
A fluorescence assay of in vivo endosome fusion was developed and applied to define the kinetics of endosome fusion in baby hamster kidney (BHK) fibroblasts. The assay is based on an approximately 10-fold enhancement of the green fluorescence of BODIPY-avidin upon biotin binding. The BODIPY-avidin fluorescence enhancement occurred in < ... More
Characterization of the mechanism of endocytic vesicle fusion in vitro.
AuthorsWessling-Resnick M, Braell WA
JournalJ Biol Chem
PubMed ID2120206
A cell-free assay to monitor receptor-mediated endocytic processes has been developed that uses biotinylated transferrin and avidin-linked beta-galactosidase as receptor-associated and fluid-phase probes, respectively (Wessling-Resnick, M., and Braell, W. A. (1990) J. Biol. Chem. 265, 690-699). The fusion of vesicles from heterologous sources can be detected in this assay: endocytic ... More
Previous studies have suggested a role for cystic fibrosis transmembrane conductance regulator (CFTR) in the regulation of intracellular vesicular trafficking. A quantitative fluorescence method was used to test the hypothesis that CFTR expression and activation affects endosome-endosome fusion in intact cells. Endosomes from CFTR-expressing and control (vector-transfected) Swiss 3T3 fibroblasts ... More
Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis.
Authors
JournalDev Cell
PubMed ID28171750
Three live-imaging techniques for comprehensively understanding the initial trigger for insulin-responsive intracellular GLUT4 trafficking.
Authors
JournaliScience
PubMed ID35434546
Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6.
Authors
JournalJ Cell Sci
PubMed ID29848659
Molecular basis of oligoubiquitin-dependent internalization of membrane proteins in Mammalian cells.
Authors
JournalTraffic
PubMed ID16497223
Loss of SNAP29 impairs endocytic recycling and cell motility.
Authors
JournalPLoS One
PubMed ID20305790
Chaperone-Independent Peripheral Quality Control of CFTR by RFFL E3 Ligase.
Authors
JournalDev Cell
PubMed ID29503157
Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection.