Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.
AuthorsVenkataraman A, Nevrivy DJ, Filtz TM, Leid M,
JournalCell Biol Int
PubMed ID22931251
GRASP interacts with Grp1 (general receptor for phosphoinositides 1; cytohesin 3), which catalyses nucleotide exchange on and activation of Arf6 (ADP-ribosylation factor-6). Arf6 is a low-molecular-mass GTPase that regulates key aspects of endocytic recycling pathways. Overexpressed GRASP accumulated in the juxtanuclear ERC (endocytic recycling compartment). GRASP co-localized with a constitutively ... More
Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor.
AuthorsHirota N, Yasuda D, Hashidate T, Yamamoto T, Yamaguchi S, Nagamune T, Nagase T, Shimizu T, Nakamura M,
JournalJ Biol Chem
PubMed ID20007715
'Several residues are conserved in the transmembrane domains (TMs) of G-protein coupled receptors. Here we demonstrate that a conserved proline, Pro(247), in TM6 of platelet-activating factor receptor (PAFR) is required for endoplasmic reticulum (ER) export and trafficking after agonist-induced internalization. Alanine-substituted mutants of the conserved residues of PAFRs, including P247A, ... More
TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
AuthorsLatz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT
JournalNat Immunol
PubMed ID14716310
'Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show ... More
Regulation of endocytosis via the oxygen-sensing pathway.
AuthorsWang Y, Roche O, Yan MS, Finak G, Evans AJ, Metcalf JL, Hast BE, Hanna SC, Wondergem B, Furge KA, Irwin MS, Kim WY, Teh BT, Grinstein S, Park M, Marsden PA, Ohh M,
JournalNat Med
PubMed ID19252501
'Tumor hypoxia is associated with disease progression, resistance to conventional cancer therapies and poor prognosis. Hypoxia, by largely unknown mechanisms, leads to deregulated accumulation of and signaling via receptor tyrosine kinases (RTKs) that are critical for driving oncogenesis. Here, we show that hypoxia or loss of von Hippel-Lindau protein--the principal ... More
Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic.
AuthorsProvance DW, Gourley CR, Silan CM, Cameron LC, Shokat KM, Goldenring JR, Shah K, Gillespie PG, Mercer JA
JournalProc Natl Acad Sci U S A
PubMed ID14766983
'Selective, in situ inhibition of individual unconventional myosins is a powerful approach to determine their specific physiological functions. Here, we report the engineering of a myosin Vb mutant that still hydrolyzes ATP, yet is selectively sensitized to an N(6)-substituted ADP analog that inhibits its activity, causing it to remain tightly ... More
Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
AuthorsPellinen T, Arjonen A, Vuoriluoto K, Kallio K, Fransen JA, Ivaska J
JournalJ Cell Biol
PubMed ID16754960
'Dynamic turnover of integrin cell adhesion molecules to and from the cell surface is central to cell migration. We report for the first time an association between integrins and Rab proteins, which are small GTPases involved in the traffic of endocytotic vesicles. Rab21 (and Rab5) associate with the cytoplasmic domains ... More
Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake.
AuthorsRichard JP, Melikov K, Vives E, Ramos C, Verbeure B, Gait MJ, Chernomordik LV, Lebleu B
JournalJ Biol Chem
PubMed ID12411431
'Cellular uptake of a family of cationic cell-penetrating peptides (examples include Tat peptides and penetratin) have been ascribed in the literature to a mechanism that does not involve endocytosis. In this work we reevaluate the mechanisms of cellular uptake of Tat 48-60 and (Arg)(9). We demonstrate here that cell fixation, ... More
Clathrin is required for the function of the mitotic spindle.
AuthorsRoyle SJ, Bright NA, Lagnado L
JournalNature
PubMed ID15858577
'Clathrin has an established function in the generation of vesicles that transfer membrane and proteins around the cell. The formation of clathrin-coated vesicles occurs continuously in non-dividing cells, but is shut down during mitosis, when clathrin concentrates at the spindle apparatus. Here, we show that clathrin stabilizes fibres of the ... More
Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn.
AuthorsOber RJ, Martinez C, Vaccaro C, Zhou J, Ward ES
JournalJ Immunol
PubMed ID14764666
'The MHC class I-related receptor, FcRn, plays a central role in regulating the serum levels of IgG. FcRn is expressed in endothelial cells, suggesting that these cells may be involved in maintaining IgG levels. We have used live cell imaging of FcRn-green fluorescent protein transfected human endothelial cells to analyze ... More
Cholesterol controls lipid endocytosis through Rab11.
AuthorsTakahashi M, Murate M, Fukuda M, Sato SB, Ohta A, Kobayashi T,
JournalMol Biol Cell
PubMed ID17475773
Cellular cholesterol increases when cells reach confluency in Chinese hamster ovary (CHO) cells. We examined the endocytosis of several lipid probes in subconfluent and confluent CHO cells. In subconfluent cells, fluorescent lipid probes including poly(ethylene glycol)derivatized cholesterol, 22-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3beta-ol, and fluorescent sphingomyelin analogs were internalized to pericentriolar recycling endosomes. This accumulation ... More
Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway.
AuthorsKumari S, Borroni V, Chaudhry A, Chanda B, Massol R, Mayor S, Barrantes FJ,
JournalJ Cell Biol
PubMed ID18591431
Endocytosis of the nicotinic acetylcholine receptor (AChR) is a proposed major mechanism of neuromodulation at neuromuscular junctions and in the pathology of synapses in the central nervous system. We show that binding of the competitive antagonist alpha-bungarotoxin (alphaBTX) or antibody-mediated cross-linking induces the internalization of cell surface AChR to late ... More
Alzheimer's disease beta-amyloid peptides are released in association with exosomes.
AuthorsRajendran L, Honsho M, Zahn TR, Keller P, Geiger KD, Verkade P, Simons K
JournalProc Natl Acad Sci U S A
PubMed ID16837572
Although the exact etiology of Alzheimer's disease (AD) is a topic of debate, the consensus is that the accumulation of beta-amyloid (Abeta) peptides in the senile plaques is one of the hallmarks of the progression of the disease. The Abeta peptide is formed by the amyloidogenic cleavage of the amyloid ... More
Endocytosis resumes during late mitosis and is required for cytokinesis.
AuthorsSchweitzer JK, Burke EE, Goodson HV, D'Souza-Schorey C
JournalJ Biol Chem
PubMed ID16207714
Recent work has underscored the importance of membrane trafficking events during cytokinesis. For example, targeted membrane secretion occurs at the cleavage furrow in animal cells, and proteins that regulate endocytosis also influence the process of cytokinesis. Nonetheless, the prevailing dogma is that endosomal membrane trafficking ceases during mitosis and resumes ... More
Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility.
AuthorsHsu T, Adereth Y, Kose N, Dammai V
JournalJ Biol Chem
PubMed ID16505488
The tumor suppressor VHL (von Hippel-Lindau protein) serves as a negative regulator of hypoxia-inducible factor-alpha subunits. However, accumulated evidence indicates that VHL may play additional roles in other cellular functions. We report here a novel hypoxia-inducible factor-independent function of VHL in cell motility control via regulation of fibroblast growth factor ... More
Heterodimerization of endothelin-converting enzyme-1 isoforms regulates the subcellular distribution of this metalloprotease.
AuthorsMuller L, Barret A, Etienne E, Meidan R, Valdenaire O, Corvol P, Tougard C
JournalJ Biol Chem
PubMed ID12393864
Endothelin-converting enzyme (ECE) is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific ... More
Real-time imaging of green fluorescent protein-tagged beta 2-adrenergic receptor distribution in living cells.
AuthorsSunaguchi M, Nishi M, Mizobe T, Kawata M
JournalBrain Res
PubMed ID12932836
In an attempt to investigate the subcellular trafficking of beta(2)-adrenergic receptor (beta(2)AR) in living cells, we performed real-time imaging of beta(2)AR tagged with green fluorescent protein (GFP). We transiently transfected a chimera construct of beta(2)AR and GFP (beta(2)AR-GFP) into HEK 293 cells, primary cultured rat hippocampal neurons and cortical neuronal ... More
Effects of Bafilomycin A1: an inhibitor of vacuolar H (+)-ATPases on endocytosis and apoptosis in RAW cells and RAW cell-derived osteoclasts.
AuthorsXu J, Feng HT, Wang C, Yip KH, Pavlos N, Papadimitriou JM, Wood D, Zheng MH
JournalJ Cell Biochem
PubMed ID12647307
Bafilomycin A1, a specific inhibitor of V-ATPases, is a potent inhibitor of bone resorption, but the underlying mechanisms of its action remain unclear. In this study, we investigated the effect of Bafilomycin A1 on endocytosis and apoptosis in RAW cells and RAW cell-derived osteoclasts. Quantitative analysis by flow cytometry showed ... More
The LMTK1-TBC1D9B-Rab11A Cascade Regulates Dendritic Spine Formation via Endosome Trafficking.
Authors
JournalJ Neurosci
PubMed ID31628178
PI3KC2β inactivation stabilizes VE-cadherin junctions and preserves vascular integrity.
Authors
JournalEMBO Rep
PubMed ID33880878
M98K-OPTN induces transferrin receptor degradation and RAB12-mediated autophagic death in retinal ganglion cells.
Authors
JournalAutophagy
PubMed ID23357852
Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections.
Authors
JournalNat Commun
PubMed ID35091550
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Authors
JournalNat Commun
PubMed ID32152276
Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling.
Authors
JournalCell Rep
PubMed ID33691102
Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.
Authors
JournalElife
PubMed ID34018922
Cooperativity of membrane-protein and protein-protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis.
Authors
JournalCell Mol Life Sci
PubMed ID32997199
Loss of Christianson Syndrome Na+/H+ Exchanger 6 (NHE6) Causes Abnormal Endosome Maturation and Trafficking Underlying Lysosome Dysfunction in Neurons.