Tubulin Tracker™ Deep Red
Tubulin Tracker™ Deep Red
Invitrogen™

Tubulin Tracker™ Deep Red

Tubulin Tracker Deep Red 在活细胞中与聚合微管蛋白结合后可发出深红色/远红外荧光。Tubulin Tracker Deep Red 基于与明亮了解更多信息
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货号数量
T34076300张载玻片
T3407760张载玻片
货号 T34076
价格(CNY)
7,313.00
300 slides
添加至购物车
数量:
300张载玻片
价格(CNY)
7,313.00
300 slides
添加至购物车
Tubulin Tracker Deep Red 在活细胞中与聚合微管蛋白结合后可发出深红色/远红外荧光。Tubulin Tracker Deep Red 基于与明亮、光稳定的深红色荧光基团结合的多西紫杉醇。多西紫杉醇属于靶向微管蛋白的细胞骨架药物家族成员。Tubulin Tracker Deep Red 理想的吸收和发射光谱分别为652 nm 和669 nm,并且几乎可以使用任何荧光成像仪器与标准 Cy5滤光片组成像。其可配合蓝色、绿色、橙色、红色和近红外荧光基团进行多重分析。
仅供科研使用。不可用于诊断程序。
规格
激发波长范围652⁄669
产品线TubulinTracker™
数量300张载玻片
运输条件经批准可置于湿冰或干冰上运输
标签类型紫外-可见标记
产品类型Tubulin Tracker
亚细胞定位微管蛋白Tubulin
Unit Size300 slides
内容与储存
含有的材料足够用于至少300张载玻片,每张载玻片使用 1 mL。

  • 5样品瓶 Tubulin Tracker Deep Red(添加 60 µL/样品瓶无水 DMSO,制备 1000X (1 mM) 储备液。 按照 1:1000 比例将储备液溶于细胞成像溶液中以制备 1X (1 µM) 染色溶液。)
    • 5 x 77 mg 丙磺舒(将样品瓶内容物溶于 1 mL 活细胞兼容缓冲液中,以制备 100X 储备液。)

      接收后,储存在 ≤–20°C 下。
      干燥、避光储存。
      根据指示储存时,产品可保持稳定至少6个月。

常见问题解答 (FAQ)

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is Tubulin Tracker Deep Red acetylated? Does it require intracellular esterase activity to make the reagent active?

Tubulin Tracker Deep Red is not acetylated. It is inherently cell permeable and is not modified further by any cellular enzymatic activity to fluoresce or bind to actin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do I need to use Probenecid when using Tubulin Tracker Deep Red?

The use of Probenecid is optional. Its requirement is dependent upon the cell type. Some cell types actively efflux the reagent out of the cells within a short time, limiting detection, other cell types do not exhibit this activity or exhibit very limited activity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.