TC-ReAsH™ II 细胞内四半胱氨酸标签检测试剂盒（红色荧光），用于活细胞成像

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引用和文献 (36)

Invitrogen™

TC-ReAsH™ II 细胞内四半胱氨酸标签检测试剂盒（红色荧光），用于活细胞成像

TC-ReAsH™ II 细胞内四半胱氨酸标签检测试剂盒含有一种用于活细胞标记的基于表达标签的荧光标记试剂。可使用试剂盒中含有的红色荧光 ReAsH-EDT2 试剂标记表达与四半胱氨酸标签 (CCPGCC) 融合的蛋白的哺乳动物细胞系。只有在加入标记试剂时，标签蛋白才会发荧光。BAL了解更多信息

货号	数量
T34562	1 Kit

货号 T34562

价格（CNY)

13,484.00

Ends: 31-Dec-2025

18,159.00

共减 4,675.00 (26%)

1 kit

添加至购物车

1 Kit

价格（CNY)

13,484.00

Ends: 31-Dec-2025

18,159.00

共减 4,675.00 (26%)

1 kit

添加至购物车

TC-ReAsH™ II 细胞内四半胱氨酸标签检测试剂盒含有一种用于活细胞标记的基于表达标签的荧光标记试剂。可使用试剂盒中含有的红色荧光 ReAsH-EDT₂ 试剂标记表达与四半胱氨酸标签 (CCPGCC) 融合的蛋白的哺乳动物细胞系。只有在加入标记试剂时，标签蛋白才会发荧光。BAL 洗涤缓冲液替代先前提供的分散蓝 3 和基于 EDT 的洗涤缓冲液，是一种气味更令人满意且产生出色信噪比的试剂。

该试剂盒含有 ReAsH-EDT₂ 标记试剂（避光储存在 -20°C 下）和 BAL 洗涤缓冲液（储存在 4°C 下）。根据指示储存时，试剂盒可保持稳定 6 个月。

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）荧光显微镜

标签或染料ReAsH

产品线TC-ReAsH II

产品类型四半胱氨酸标签检测试剂盒

数量1 Kit

运输条件湿冰

产品规格试剂盒

Unit Size1 kit

内容与储存

在冷冻冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

Can fluorescent protein-expressing cells be fixed?

Yes, fluorescent protein-expressing cells can be fixed using 4% paraformaldehyde in PBS for 10 min followed by one quick PBS rinse and 3 x 5 min washes with 1 mL PBS.

Are the fluorescent proteins offered by Thermo Fisher Scientific (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) humanized?

Yes, all of the fluorescent proteins offered by (EmGFP, YFP, CFP, BFP and Cycle 3 GFP) have been humanized for optimal mammalian expression.

引用和文献 (36)

引用和文献

Abstract

Resolution of de novo HIV production and trafficking in immature dendritic cells.

Authors:Turville SG, Aravantinou M, Stössel H, Romani N, Robbiani M,

Journal:Nat Methods

PubMed ID:18059278

'The challenge in observing de novo virus production in human immunodeficiency virus (HIV)-infected dendritic cells (DCs) is the lack of resolution between cytosolic immature and endocytic mature HIV gag protein. To track HIV production, we developed an infectious HIV construct bearing a diothiol-resistant tetracysteine motif (dTCM) at the C terminus ... More

Identification of an intracellular trafficking and assembly pathway for HIV-1 gag.

Authors:Perlman M, Resh MD

Journal:Traffic

PubMed ID:16683918

'Retroviral Gag proteins are membrane-bound polyproteins that are necessary and sufficient for virus-like particle (VLP) formation. It is not known how Gag traffics through the cell or how the site of particle production is determined. Here we use two techniques, biarsenical/tetracysteine (TC) labeling and release from a cycloheximide block, to ... More

Real-time visualization of HIV-1 GAG trafficking in infected macrophages.

Authors:Gousset K, Ablan SD, Coren LV, Ono A, Soheilian F, Nagashima K, Ott DE, Freed EO,

Journal:PLoS Pathog

PubMed ID:18369466

'HIV-1 particle production is driven by the Gag precursor protein Pr55(Gag). Despite significant progress in defining both the viral and cellular determinants of HIV-1 assembly and release, the trafficking pathway used by Gag to reach its site of assembly in the infected cell remains to be elucidated. The Gag trafficking ... More

Site-specific, orthogonal labeling of proteins in intact cells with two small biarsenical fluorophores.

Authors:Zürn A, Klenk C, Zabel U, Reiner S, Lohse MJ, Hoffmann C,

Journal:Bioconjug Chem

PubMed ID:20429545

'The fusion of fluorescent proteins to proteins of interest has greatly advanced fluorescence microscopy, but is often limited by their large size. Here, we report site-specific, orthogonal labeling of two cellular proteins in intact cells with two small fluorescent dyes: fluorescein arsenical hairpin binder, FlAsH, and its red analogue, ReAsH, ... More

Fluorescence imaging of amyloid formation in living cells by a functional, tetracysteine-tagged alpha-synuclein.

Authors:Roberti MJ, Bertoncini CW, Klement R, Jares-Erijman EA, Jovin TM

Journal:Nat Methods

PubMed ID:17351621

'Alpha-synuclein is a major component of intraneuronal protein aggregates constituting a distinctive feature of Parkinson disease. To date, fluorescence imaging of dynamic processes leading to such amyloid deposits in living cells has not been feasible. To address this need, we generated a recombinant alpha-synuclein (alpha-synuclein-C4) bearing a tetracysteine target for ... More

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