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Citations & References (19)
Invitrogen™
Transferrin From Human Serum, Alexa Fluor™ 555 Conjugate
Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cellsRead more
| Catalog Number | Quantity |
|---|---|
| T35352 | 5 mg |
Catalog number T35352
Price (CNY)
8,876.00
5 mg
Quantity:
5 mg
Price (CNY)
8,876.00
5 mg
Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.
These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.
Transferrin Specifications:
• Label (Ex/Em): Alexa Fluor™ 555 (555/565)
• Amount: 15 mg solid (contains 5 mg of transferrin conjugate)
Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.
Transferrin Specifications:
• Label (Ex/Em): Alexa Fluor™ 555 (555/565)
• Amount: 15 mg solid (contains 5 mg of transferrin conjugate)
Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeAlexa Fluor Dyes
Excitation/Emission555/565
FormSolid
Protein FamilyTransferrin
Quantity5 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor
Product TypeTransferrin
pH7.2
Unit Size5 mg
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (19)
Citations & References
Abstract
TrkA receptor activation by nerve growth factor induces shedding of the p75 neurotrophin receptor followed by endosomal gamma-secretase-mediated release of the p75 intracellular domain.
Journal:J Biol Chem
PubMed ID:17215246
'Neurotrophins are trophic factors that regulate important neuronal functions. They bind two unrelated receptors, the Trk family of receptor-tyrosine kinases and the p75 neurotrophin receptor (p75). p75 was recently identified as a new substrate for gamma-secretase-mediated intramembrane proteolysis, generating a p75-derived intracellular domain (p75-ICD) with signaling capabilities. Using PC12 cells
VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes.
Journal:J Cell Sci
PubMed ID:17327277
'VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN, but its trafficking itinerary is unknown. Cells stably expressing TGN-enriched VAMP4 C-terminally-tagged with EGFP (VAMP4-EGFP) are able to internalize and transport EGFP antibody efficiently to the TGN, suggesting that
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17384151
'The intracellular events on the recycling pathway that lead from sorting endosomes to exocytosis at the plasma membrane are central to cellular function. However, despite intensive study, these processes are poorly characterized in spatial and dynamic terms. The primary reason for this is that, to date, it has not been
Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
Journal:J Cell Biol
PubMed ID:16754960
'Dynamic turnover of integrin cell adhesion molecules to and from the cell surface is central to cell migration. We report for the first time an association between integrins and Rab proteins, which are small GTPases involved in the traffic of endocytotic vesicles. Rab21 (and Rab5) associate with the cytoplasmic domains
High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells.
Journal:Biophys J
PubMed ID:18835896
'Single particle tracking in three dimensions in a live cell environment holds the promise of revealing important new biological insights. However, conventional microscopy-based imaging techniques are not well suited for fast three-dimensional (3D) tracking of single particles in cells. Previously we developed an imaging modality multifocal plane microscopy (MUM) to