FM™ 1-43染料（N-(3-三乙基铵丙基)-4-(4-(二丁基氨基)苯乙烯基)吡啶二溴盐）
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
FM&trade; 1-43染料（<i>N</i>-(3-三乙基铵丙基)-4-(4-(二丁基氨基)苯乙烯基)吡啶二溴盐）
Invitrogen™

FM™ 1-43染料（N-(3-三乙基铵丙基)-4-(4-(二丁基氨基)苯乙烯基)吡啶二溴盐）

FM 1-43膜探针是一种出色的试剂，可用于鉴定主动应激神经元，研究活性依赖性囊泡循环机制。这种水溶性染料对细胞无毒且在水溶介质中几乎不发荧光，在插入细胞膜外层中后发出明亮的荧光。在主动释放神经递质的神经元中，染料会在可回收突触囊泡中内化，导致神经末端明亮染色。在成像之前，细胞表面膜的非特异性染色可通过洗涤轻松去除。FM 1-43膜探针也可以1 mg 形式封装在单个小瓶中 (T-3163)了解更多信息
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货号数量
T3535610 x 100 μg
T31631 mg
货号 T35356
价格（CNY)
6,309.00
飞享价
Ends: 31-Dec-2025
8,552.00
共减 2,243.00 (26%)
10 x 100 µg
添加至购物车
数量:
10 x 100 μg
价格（CNY)
6,309.00
飞享价
Ends: 31-Dec-2025
8,552.00
共减 2,243.00 (26%)
10 x 100 µg
添加至购物车
FM 1-43膜探针是一种出色的试剂，可用于鉴定主动应激神经元，研究活性依赖性囊泡循环机制。这种水溶性染料对细胞无毒且在水溶介质中几乎不发荧光，在插入细胞膜外层中后发出明亮的荧光。在主动释放神经递质的神经元中，染料会在可回收突触囊泡中内化，导致神经末端明亮染色。在成像之前，细胞表面膜的非特异性染色可通过洗涤轻松去除。FM 1-43膜探针也可以1 mg 形式封装在单个小瓶中 (T-3163)。还可提供 FM 1-43FX 膜探针(F-35355)，后者是可使用醛类固定剂固定的 FM 1-43膜探针的模拟物。
仅供科研使用。不可用于诊断程序。
规格
颜色红色
检测方法荧光
适用于（设备）荧光显微镜
产品线FM™
数量10 x 100 μg
运输条件室温
标签类型Fluorescent Dye
产品类型染料
亚细胞定位细胞膜
Unit Size10 x 100 µg
内容与储存
室温避光储存。

常见问题解答 (FAQ)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.