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引用和文献 (15)
Invitrogen™
硫醇和硫化物定量试剂盒
硫醇和硫化物定量试剂盒提供了一种超灵敏的比色测定法，可用于对蛋白质和非蛋白质硫醇进行定量。在该检测中，硫醇或无机硫化物会还原木瓜蛋白酶的二硫化物抑制衍生物，并且以化学计量方式释放活性酶。然后，使用显色木瓜蛋白酶底物 N-苯甲酰-L-精氨酸 p-硝基苯胺 (L-BAPNA) 测量酶活性。该试剂盒的酶促扩增步骤使研究人员能够检测到低至 0.2 μM了解更多信息
| 货号 | 数量 |
|---|---|
| T6060 | 1 Kit |
货号 T6060
价格（CNY)
5,275.00
1 kit
数量:
1 Kit
价格（CNY)
5,275.00
1 kit
硫醇和硫化物定量试剂盒提供了一种超灵敏的比色测定法，可用于对蛋白质和非蛋白质硫醇进行定量。在该检测中，硫醇或无机硫化物会还原木瓜蛋白酶的二硫化物抑制衍生物，并且以化学计量方式释放活性酶。然后，使用显色木瓜蛋白酶底物 N-苯甲酰-L-精氨酸 p-硝基苯胺 (L-BAPNA) 测量酶活性。该试剂盒的酶促扩增步骤使研究人员能够检测到低至 0.2 μM 硫醇，其灵敏度比使用 Ellman 试剂高出约 100 倍。
仅供科研使用。不可用于诊断程序。
规格
数量1 Kit
产品类型硫醇和硫化物定量试剂盒
Unit Size1 kit
引用和文献 (15)
引用和文献
Abstract
Site-directed fluorescence studies of a prokaryotic ClC antiporter.
Journal:Biochemistry
PubMed ID:16734414
'Channels and transporters of the ClC family serve a variety of physiological functions. Understanding of their gating and transport mechanisms remains incomplete, with disagreement over the extent of protein conformational change involved. Using site-directed fluorescence labeling, we probe ClC-ec1, a prokaryotic ClC, for transport-related structural rearrangements. We specifically label cysteines
Reversible glutathionylation of complex I increases mitochondrial superoxide formation.
Journal:J Biol Chem
PubMed ID:12649289
'Increased production of reactive oxygen species (ROS) by mitochondria is involved in oxidative damage to the organelle and in committing cells to apoptosis or senescence, but the mechanisms of this increase are unknown. Here we show that ROS production by mitochondrial complex I increases in response to oxidation of the
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular
A sensitive assay for maleimide groups.
Journal:Bioconjug Chem
PubMed ID:7948101
A sensitive spectrophotometric assay has been developed for maleimide groups incorporated into proteins. The assay involves the reaction of maleimide with an excess of cysteine and quantitation of the remaining cysteine using an enzymatic assay that is based on the stoichiometric conversion of an inactive disulfide form of papain to
An amplified assay for thiols based on reactivation of papain.
Journal:Anal Biochem
PubMed ID:8238881
A sensitive spectrophotometric assay has been developed for thiol (sulfhydryl) groups using an inactive disulfide derivative of papain (papain-S-SCH3). The thiol-disulfide interchange reaction of a thiol with papain-S-SCH3 results in the stoichiometric formation of active papain (papain-SH). The reactivated papain catalyzes the hydrolysis of a chromogenic substrate, resulting in an