Cellular internalization of poly(ethylene oxide)-b-poly(epsilon-caprolactone) diblock copolymer micelles.
AuthorsLuo L, Tam J, Maysinger D, Eisenberg A
JournalBioconjug Chem
PubMed ID12440861
'Poly(ethylene oxide)-b-poly(epsilon-caprolactone) (PEO-b-PCL) block copolymers self-assemble into micelles in aqueous solution. We have examined whether these micelles can internalize into P19 cells in vitro. Fluorescently labeled PEO(45)-b-PCL(23) block copolymer was prepared by conjugating a tetramethylrhodamine molecule to the end of the hydrophobic PCL block. Dynamic light scattering (DLS) and transmission ... More
Micellar nanocontainers distribute to defined cytoplasmic organelles.
AuthorsSavic R, Luo L, Eisenberg A, Maysinger D
JournalScience
PubMed ID12714738
Block copolymer micelles are water-soluble biocompatible nanocontainers with great potential for delivering hydrophobic drugs. An understanding of their cellular distribution is essential to achieving selective delivery of drugs at the subcellular level. Triple-labeling confocal microscopy in live cells revealed the localization of micelles in several cytoplasmic organelles, including mitochondria, but ... More
A novel rhodamine-riboflavin conjugate probe exhibits distinct fluorescence resonance energy transfer that enables riboflavin trafficking and subcellular localization studies.
Riboflavin (vitamin B2, RF) is taken up in eukaryotic cells via specialized transport mechanisms. Although RF has fluorescence properties, direct microscopic visualization of RF uptake and trafficking has been complicated by cellular autofluorescence. We describe the synthesis, cellular uptake characteristics, and spectroscopic properties of a novel rhodamine-riboflavin conjugate (RD-RF), including ... More
Involvement of endocytic organelles in the subcellular trafficking and localization of riboflavin.
AuthorsHuang SN, Phelps MA, Swaan PW
JournalJ Pharmacol Exp Ther
PubMed ID12721324
Previous studies by our laboratory have suggested the potential role of receptor-mediated endocytosis components in the cellular translocation of riboflavin (vitamin B2). To delineate the intracellular compartments and events involved in the internalization of riboflavin, we synthesized a rhodamine-labeled riboflavin conjugate to monitor its movement via fluorescent microscopy. Cellular uptake ... More