四甲基罗丹明,乙酯,高氯酸盐 (TMRE)
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四甲基罗丹明,乙酯,高氯酸盐 (TMRE)
Invitrogen™

四甲基罗丹明,乙酯,高氯酸盐 (TMRE)

四甲基罗丹明乙酯 (TMRE) 是一种细胞通透性、阳离子型橙色荧光染料,可轻松吸附活性线粒体了解更多信息
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货号数量
T66925 mg
货号 T669
价格(CNY)
2,559.00
飞享价
Ends: 31-Dec-2025
3,467.00
共减 908.00 (26%)
25 mg
添加至购物车
数量:
25 mg
价格(CNY)
2,559.00
飞享价
Ends: 31-Dec-2025
3,467.00
共减 908.00 (26%)
25 mg
添加至购物车
四甲基罗丹明乙酯 (TMRE) 是一种细胞通透性、阳离子型橙色荧光染料,可轻松吸附活性线粒体。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
激发/发射549/574 nm
分子量514.96
数量25 mg
运输条件室温
子细胞定位线粒体
颜色橙红色
适用于(设备)荧光显微镜
产品类型TMRE
Unit Size25 mg
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时,看到神经元周围出现了较高的背景,如何降低背景干扰?

如果使用我们的FluoVolt 膜电势试剂盒(货号F10488),该试剂盒包含一种背景抑制剂,可改善这一问题。对于其他指标剂,可以考虑使用BackDrop 背景抑制剂(货号R37603、B10511和B10512)。

快反应膜电位探针和慢反应膜电位探针有什么区别?

在周围电场的作用下结构变化的分子可用作检测瞬时(毫秒级)电位变化的快反应探针。慢反应染料则会进入去极化细胞,结合蛋白或膜。增强去极化会造成额外的染料流入,增强荧光强度;过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像,或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂?我该如何根据自己的试验选择?

膜电位指示剂选择指南请见此处(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html)。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (159)

引用和文献
Abstract
A hyperfused mitochondrial state achieved at G1-S regulates cyclin E buildup and entry into S phase.
Authors:Mitra K, Wunder C, Roysam B, Lin G, Lippincott-Schwartz J,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19617534
Mitochondria undergo fission-fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G(1)-S boundary. Mitochondria convert from isolated, fragmented elements into a hyperfused, giant network at G(1)-S transition. The network is electrically continuous and has greater ATP ... More
Antiapoptotic effect of nicorandil mediated by mitochondrial atp-sensitive potassium channels in cultured cardiac myocytes.
Authors:Akao M, Teshima Y, Marbán E
Journal:J Am Coll Cardiol
PubMed ID:12204514
OBJECTIVES: We examined whether nicorandil, a clinically useful drug for the treatment of ischemic syndromes, inhibits myocardial apoptosis. BACKGROUND: Nicorandil has been reported to have a cardioprotective action through activation of mitochondrial ATP-sensitive potassium (mitoK(ATP)) channels. Based on our recent observation that mitoK(ATP) channel activation has a remarkable antiapoptotic effect ... More
Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria.
Authors:Brown MR, Sullivan PG, Geddes JW
Journal:J Biol Chem
PubMed ID:16517608
'Mitochondria in nerve terminals are subjected to extensive Ca2+ fluxes and high energy demands, but the extent to which the synaptic mitochondria buffer Ca2+ is unclear. In this study, we identified a difference in the Ca2+ clearance ability of nonsynaptic versus synaptic mitochondrial populations enriched from rat cerebral cortex. Mitochondria ... More
Age-related macular degeneration. The lipofusion component N-retinyl-N-retinylidene ethanolamine detaches proapoptotic proteins from mitochondria and induces apoptosis in mammalian retinal pigment epithelial cells.
Authors:Suter M, Remé C, Grimm C, Wenzel A, Jäättela M, Esser P, Kociok N, Leist M, Richter C
Journal:J Biol Chem
PubMed ID:11006290
'10-20% of individuals over the age of 65 suffer from age-related macular degeneration (AMD), the leading cause of severe visual impairment in humans living in developed countries. The pathogenesis of this complex disease is poorly understood, and no efficient therapy or prevention exists to date. A precondition for AMD appears ... More
VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome c release.
Authors:Madesh M, Hajnóczky G
Journal:J Cell Biol
PubMed ID:11739410
'Enhanced formation of reactive oxygen species (ROS), superoxide (O2*-), and hydrogen peroxide (H2O2) may result in either apoptosis or other forms of cell death. Here, we studied the mechanisms underlying activation of the apoptotic machinery by ROS. Exposure of permeabilized HepG2 cells to O2*- elicited rapid and massive cytochrome c ... More