Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.
AuthorsMichalet X, Lacoste TD, Weiss S
JournalMethods
PubMed ID11559000
'An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the ... More
Ultrahigh-resolution multicolor colocalization of single fluorescent probes.
An optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described in this paper. It relies on the use of two unique families of fluorophores, namely energy-transfer fluorescent beads (TransFluoSpheres) and semiconductor nanocrystal quantum dots, that can be excited by a single laser wavelength but emit at different ... More