Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.
AuthorsMichalet X, Lacoste TD, Weiss S
JournalMethods
PubMed ID11559000
'An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the ... More
Development and cellular applications of fiber optic nitric oxide sensors based on a gold-adsorbed fluorophore.
AuthorsBarker SL, Kopelman R
JournalAnal Chem
PubMed ID9852778
A new design for optochemical sensors has been applied to the development of a nitric oxide selective fiber optic sensor. This sensor is composed of a fluorescein derivative dye attached to colloidal gold. The fluorescein dye rearranges as nitric oxide adsorbs onto the gold, inducing a decrease in the fluorescence ... More
Ultrahigh-resolution multicolor colocalization of single fluorescent probes.
An optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described in this paper. It relies on the use of two unique families of fluorophores, namely energy-transfer fluorescent beads (TransFluoSpheres) and semiconductor nanocrystal quantum dots, that can be excited by a single laser wavelength but emit at different ... More
Cellular applications of a sensitive and selective fiber-optic nitric oxide biosensor based on a dye-labeled heme domain of soluble guanylate cyclase.
AuthorsBarker SL, Zhao Y, Marletta MA, Kopelman R
JournalAnal Chem
PubMed ID10366889
Nitric oxide-selective sensors have been prepared with the heme domain of soluble guanylate cyclase (sGC), the only known receptor for signal transduction involving nitric oxide. Expressed in and purified from E. coli, the heme domain contains a stoichiometric amount of heme that has electronic and resonance Raman spectra almost identical ... More