Determination of in situ bacterial growth rates in aquifers and aquifer sediments.
AuthorsMailloux BJ, Fuller ME
JournalAppl Environ Microbiol
PubMed ID12839747
Laboratory and field-scale studies with stained cells were performed to monitor cell growth in groundwater systems. During cell division, the fluorescence intensity of the protein stain 5-(and 6-)-carboxyfluorescein diacetate succinimidyl ester (CFDA/SE) for each cell is halved, and the intensity can be tracked with a flow cytometer. Two strains of ... More