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UTW Tubes, 0.1 mL, with flat caps, white - FAQs

查看更多产品信息 UTW Tubes, 0.1 mL, with flat caps, white - FAQs (TUC0011)

7 个常见问题解答

When should I use a Thermo Scientific low profile PCR plastic?

We recommend Thermo Scientific low profile PCR plates and tubes for reaction volumes below 20 µL. The low profile design minimizes the airspace between the PCR reaction and reduces evaporation effects, thereby increasing PCR efficiency in low volume reactions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is a low profile Thermo Scientific plate/tube?

Low profile Thermo Scientific PCR plastics have shorter wells than the standard profile plastics, decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why should I use Thermo Scientific white plastics in qPCR?

White plates and tubes reflect significantly more signal back to the qPCR instrument's detector than traditional clear plates. The improved signal reflection ensures that even the lowest levels of fluorescence are detected. White well walls also prevent signal from passing through to the cycler block where it can be inconsistently reflected or absorbed. This keeps variations in the cycler block from affecting qPCR data. Using Thermo Scientific white PCR plastics increases sensitivity and reduces variability within the qPCR assay.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Are ultra thin wall (UTW) vessels compatible with real-time experiments?

Yes. All of the UTW plastics we provide are well suited for qPCR reactions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Will ultra thin wall (UTW) vessels automatically speed up reactions on my conventional thermal cycler?

To realize the time savings of the UTW plastics on conventional thermal cyclers, optimization of the protocol is required. This typically involves reducing hold times and changing the actual hold temperatures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Are ultra thin wall (UTW) vessels prone to break more often because of their thinner walls?

When used as instructed, these vessels are remarkably robust and can handle typical laboratory conditions, without fracturing or resulting in loss of volume. It is recommended that, when applying caps, a proper tray be used to protect the bottom of the tubes from excessive force that is typically used.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Which plastic consumables should I use on my real-time PCR (qPCR)/thermal cycler (PCR) instrument?

Please select your real-time PCR (qPCR) or thermal cycler (PCR) instrument model from the drop-down menu in the qPCR and PCR Plastics Selection Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html) to see the list of all validated plastic consumables (plates, tube strips, single tubes, caps, seals, adhesive films, and accessories) we offer that are compatible with your instrument.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.