Quantitative screening of single copies of human papilloma viral DNA without amplification.
AuthorsLi J, Lee JY, Yeung ES
JournalAnal Chem
PubMed ID16970325
'We describe a novel quantitative viral screening method based on single-molecule detection that does not require amplification. DNA of human papilloma virus (HPV), the major etiological agent of cervical cancer, served as the screening target in this study. Eight 100-nucleotide single-stranded DNA probes were designed complementary to the E6-E7 gene ... More
The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia.
AuthorsKazama Y, Sugiyama R, Suto Y, Uchida W, Kawano S
JournalGenome
PubMed ID16767177
The satellite DNA (satDNA) on the ends of chromosomes has been isolated and characterized in the dioecious plant Silene latifolia. BAC clones containing large numbers of repeat units of satDNA in a tandem array were isolated to examine the clustering of the repeat units. satDNA repeat units were purified from ... More
Enhanced nucleic acid capture and flow cytometry detection with peptide nucleic acid probes and tunable-surface microparticles.
AuthorsChandler DP, Jarrell AE
JournalAnal Biochem
PubMed ID12531204
New methods for automated, direct nucleic acid purification and detection are required for the next generation of unattended environmental monitoring devices. In this study we investigated whether tunable-surface bead chemistry and peptide nucleic acids (PNA) could enhance the recovery and detection of intact rRNA in both test tube and automated ... More