Non-isotopic labeling of DNA by newly developed hapten-containing platinum compounds.
Authorsvan Belkum A, Linkels E, Jelsma T, van den Berg FM, Quint W
JournalBiotechniques
PubMed ID7639838
A newly developed reagent was tested for its applicability in in situ hybridization and in reversed hybridization of DNA fragments generated by PCR amplification. This Platinum-complex, designated universal linkage system (ULS), equipped, for instance, with biotin or fluorescein as hapten, enables versatile nonenzymatic "one step" labeling of genomic, cloned or ... More
Trafficking and localization of platinum complexes in cisplatin-resistant cell lines monitored by fluorescence-labeled platinum.
Cisplatin is a chemotherapeutic agent commonly used in the treatment of a wide variety of malignant tumors. Resistance to cisplatin represents a major obstacle to effective cancer therapy because clinically significant levels of resistance quickly emerge after treatment. Based on previous studies indicating abnormal plasma membrane protein trafficking in cisplatin-resistant ... More
Probing alternative foldings of the HIV-1 leader RNA by antisense oligonucleotide scanning arrays.
AuthorsOoms M, Verhoef K, Southern E, Huthoff H, Berkhout B
JournalNucleic Acids Res
PubMed ID14762209
Scanning arrays of antisense DNA oligonucleotides provide a novel and systematic means to study structural features within an RNA molecule. We used this approach to probe the structure of the untranslated leader of the human immunodeficiency virus type 1 (HIV-1) RNA genome. This 335 nt RNA encodes multiple important replication ... More
DNA fiber-FISH staining mechanism.
Authorsvan de Rijke FM, Florijn RJ, Tanke HJ, Raap AK
JournalJ Histochem Cytochem
PubMed ID10820147
Fluorescence in situ hybridization to DNA fibers (Fiber-FISH) is a high-resolution, wide-ranging physical DNA mapping method that finds increasing application in the study of pathological gene rearrangements. Here we present experiments designed to understand the nature of the discontinuous FISH signal patterns seen after Fiber-FISH. Use of a novel cisplatin-based ... More
Dissecting the maize genome by using chromosome addition and radiation hybrid lines.
We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is available only in haploid oat background. Most of the maize ... More
Dendritic nucleic acid structures.
AuthorsNilsen TW, Grayzel J, Prensky W
JournalJ Theor Biol
PubMed ID9237897
Dendritic molecules are highly-branched arborescent structures and have found applications as chemical reagents, lubricants, contrast media for magnetic resonance, and others. Dendritic nucleic acids could be extremely useful for the development of nucleic acid diagnostics as signal amplification tools and potentially as drug (antisense) delivery vehicles. Further, due to the ... More